CIDEA-dependent lipid droplet induction in C. elegans
Lead Research Organisation:
Imperial College London
Department Name: Surgery and Cancer
Abstract
United States
People |
ORCID iD |
| Mark Christian (Principal Investigator) |
Publications
Schilperoort M
(2018)
The GPR120 agonist TUG-891 promotes metabolic health by stimulating mitochondrial respiration in brown fat.
in EMBO molecular medicine
| Description | The visit was very successful and productive allowing the establishment of a new collaboration for the study of CIDE-dependent LD formation in yeast. From the visit we have established yeast as a model system for the study of CIDE gene function. Although Dr Barneda spent only one month in the Henry lab in Cornell he was able to validate the yeast expression model and determine the importance of PA in CIDEA action. Importantly, although the CIDEA gene has no yeast equivalent, it was fully functional in increasing lipid droplet size. |
| Exploitation Route | The finding from this visit that CIDEA expression in yeast increased lipid droplet size could be taken further in biotechnological applications. Applications include biodiesel production. |
| Sectors | Agriculture, Food and Drink,Chemicals,Energy,Pharmaceuticals and Medical Biotechnology |
| Description | UK economic competitiveness is facilitated by training and skills acquisition. The ISIS-funded lab visit has succeeded in providing an excellent training of the appointed Post Doc David Barneda. He developed molecular tools used in the study through this international visit to Cornell University where he acquired additional skills in yeast manipulations. The wide range of skills acquired from the project (e.g. time management, problem solving, information technology, presentation skills, record keeping, budgeting, innovative thinking and mentoring) are applicable to career progression both within and outside academia. Thus, David has benefitted as an individual from the training, and the UK institutions where he applies his skills and training will benefit. David is continuing his scientific career at Queen Mary University of London. The visit to Cornell University was one component of an international multidisciplinary collaboration network (UK-USA-Italy-China) established through this project has served to enhance the research capacity and skills base. The management skills involved in coordinating a multicentre collaboration are important on a personal development perspective and are applicable to economic advancement. The network has served to raise the profile of UK research with a group of influential scientists and policy makers within their different fields. |
| First Year Of Impact | 2013 |
| Impact Types | Societal,Economic |
| Description | BBSRC Responsive Mode |
| Amount | £293,592 (GBP) |
| Funding ID | BB/P005209/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 04/2017 |
| End | 03/2020 |
| Title | Yeast model of CIDEA expression |
| Description | Expression system for CIDEA in yeast cells |
| Type Of Material | Technology assay or reagent |
| Provided To Others? | No |
| Impact | In conjuction with a range of yeast strains, this tool enabled us to determine the mechanism of action of CIDEA in promoting lipid droplet enlargement. The data obtained is included in a manuscript prepared for publication. |
| Description | Investigation of CIDE genes in yeast |
| Organisation | Cornell University |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | With the assistance of a BBSRC ISIS grant, a collaboration was established with Cornell to study the action of CIDEA using yeast as a model system. David Barneda travelled to the lab of Prof Susan Henry where he performed experiments to express CIDEA in yeast cells. |
| Collaborator Contribution | Prof Susan Henry and her lab members provided input into experimental design for CIDEA expression systems in yeast. In addition, the provided yeast cell lines and expression constructs. |
| Impact | Findings were that CIDEA was fully functional in promoting large lipid droplets in yeast. This is important as yeast do not have genes related to CIDEA in their genome. The data from this collaboration are included in a manuscript being prepared for submission. |
| Start Year | 2012 |