Three dimensional cellular electron microscopy of eukaryotic basal bodies.
Lead Research Organisation:
Oxford Brookes University
Department Name: Faculty of Health and Life Sciences
Abstract
France
People |
ORCID iD |
| Sue Vaughan (Principal Investigator) |
Publications
Hughes L
(2017)
Patterns of organelle ontogeny through a cell cycle revealed by whole-cell reconstructions using 3D electron microscopy.
in Journal of cell science
| Description | This travel grant was to set up a collaboration between Institut Pasteur and Oxford Brookes University and obtain training in cyro-electron microscopy techniques. Staff visited Institut Pasteur, delivered talks and received training. We now have an active collaboration which will hopefully produce a publication and grant application based on the research. |
| Exploitation Route | Staff have received training which they can use in job applications and training of other staff.We have a good collaboration with Dr Philippe Bastin which should results in a publication. |
| Sectors | Healthcare |
| Description | BBSRC ALERT 13 |
| Amount | £748,000 (GBP) |
| Funding ID | BB/L014122/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2014 |
| End | 12/2014 |
| Description | BBSRC responsive mode |
| Amount | £611,000 (GBP) |
| Funding ID | BB/M000532/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 12/2014 |
| End | 11/2018 |
| Description | Genome scale genetic tagging and protein localisation in Trypanosoma brucei |
| Amount | £698,000 (GBP) |
| Funding ID | 108445/Z/15/Z |
| Organisation | Wellcome Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 01/2016 |
| End | 12/2019 |
| Title | serial block face scanning electron microscopy |
| Description | Our successful ALERT 13 equipment grant allowed us to purchase a 3D imaging system called serial block face-scanning electron microscopy. We have developed applications and methods to visualise whole individual trypanosome cells. We now have 9 active collaborators wishing to use the service. |
| Type Of Material | Biological samples |
| Year Produced | 2014 |
| Provided To Others? | Yes |
| Impact | Publication in Journal of Cell Science. We also have a 3D printer and have printed out individual trypanosome cells and have an exhibit at BBSRC Great British Bioscience to explain our research to the general public. |
| Description | 15th European Microscopy Conference |
| Form Of Engagement Activity | Scientific meeting (conference/symposium etc.) |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Participants in your research or patient groups |
| Results and Impact | Talk no actual impacts realised to date |
| Year(s) Of Engagement Activity | 2012 |
| Description | Conference - Trypanosome breeders club |
| Form Of Engagement Activity | Scientific meeting (conference/symposium etc.) |
| Part Of Official Scheme? | Yes |
| Primary Audience | Participants in your research or patient groups |
| Results and Impact | This is a biannual meeting of all the principle investigators in the UK who work on Trypanosomes. It enables us to hear about new work and forge collaborations and helps with grant writing I was invited to give a talk on our work from the BBSRC grant on electron tomography of basal bodies and the new technique of serial block face scanning electron microscopy. Presented BBSRC work from the grant no actual impacts realised to date |
| Year(s) Of Engagement Activity | 2013 |