Advanced iron-specific spectroscopies for the study of iron-sulfur cluster transcriptional regulators
Lead Research Organisation:
University of East Anglia
Department Name: Chemistry
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
People |
ORCID iD |
| Nicolas Le Brun (Principal Investigator) |
Publications
Crack JC
(2018)
Redox-Sensing Iron-Sulfur Cluster Regulators.
in Antioxidants & redox signaling
Crack JC
(2014)
Iron-sulfur clusters as biological sensors: the chemistry of reactions with molecular oxygen and nitric oxide.
in Accounts of chemical research
Crack JC
(2015)
NsrR from Streptomyces coelicolor is a nitric oxide-sensing [4Fe-4S] cluster protein with a specialized regulatory function.
in The Journal of biological chemistry
Serrano PN
(2016)
Nitrosylation of Nitric-Oxide-Sensing Regulatory Proteins Containing [4Fe-4S] Clusters Gives Rise to Multiple Iron-Nitrosyl Complexes.
in Angewandte Chemie (International ed. in English)
| Description | We have developed the application of the novel, iron-specific technique nuclear resonance vibrational spectroscopy (NRVS) to studies of nitric oxide sensing by a range of transcriptional regulators, including NsrR and Wbl proteins. This has provided important new insight into the mechanisms of sensing - how the sensory module (an iron-sulphur cluster) reacts with nitric oxide to form iron-nitrosyl intermediates and products. |
| Exploitation Route | Our work has shown how NRVS can be applied to proteins that play an important physiological role in relation to nitric oxide. The work could be taken forward by others studying similar or different types of iron protein. |
| Sectors | Healthcare,Pharmaceuticals and Medical Biotechnology,Other |
| Description | The Partnership award enabled exchange of people and expertise between the Le Brun and Cramer groups, and facilitated their collaboration on iron-sulfur regulatory proteins. Papers arising from this interaction have been published recently, and while there is not yet any direct non-academic impact, the insight provided has laid and is laying the foundation for this in the future. |
| Sector | Pharmaceuticals and Medical Biotechnology |
| Description | Application of nuclear resonance vibrational spectroscopy (NRVS) to studies of nitric oxide sensing in regulatory proteins |
| Organisation | University of California, Davis |
| Department | Department of Chemistry |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Supply of extremely difficult to prepare samples and intellectual input into experiment design |
| Collaborator Contribution | Technical expertise and synchrontron access for this highly specialised technique |
| Impact | Two research papers have so far resulted from this collaboration, with another currently in preparation. |
| Start Year | 2012 |
| Description | Discover Chemistry and Pharmacy |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | Yes |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | Individual questions were raised; contributed to an overall increased understanding of research. Many of the attendees of the summer school choose to study Chemistry or pharmacy at University |
| Year(s) Of Engagement Activity | 2011,2012,2013,2014,2015 |
| Description | School Visit (Lincolnshire) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | I visited a School in Lincolnshire to present an accessible but research led talk on the roles of metals in biology to a Year 12 class of approx 15 pupils. |
| Year(s) Of Engagement Activity | 2015 |