Chromosome structure workshop
Lead Research Organisation:
University College London
Department Name: London Centre for Nanotechnology
Abstract
united kingdom
Publications
Bradley RS
(2017)
3D X-Ray Nanotomography of Cells Grown on Electrospun Scaffolds.
in Macromolecular bioscience
Robinson I
(2015)
Coherent three-dimensional X-ray cryo-imaging.
in IUCrJ
Robinson I
(2016)
Nuclear incorporation of iron during the eukaryotic cell cycle.
in Journal of synchrotron radiation
Robinson I
(2015)
Towards single particle imaging of human chromosomes at SACLA
in Journal of Physics B: Atomic, Molecular and Optical Physics
Robinson Ian
(2015)
Progress towards 3D imaging of Human Metaphase Chromosomes
in CHROMOSOME RESEARCH
Yan H
(2016)
Multimodality hard-x-ray imaging of a chromosome with nanoscale spatial resolution.
in Scientific reports
Yang F
(2019)
Investigation of Three-Dimensional Structure and Pigment Surrounding Environment of a TiO2 Containing Waterborne Paint.
in Materials (Basel, Switzerland)
Yusuf M
(2016)
Future Prospects of 3D Human Chromosome Imaging by Serial Block Face Scanning Electron Microscopy
in Single Cell Biology
Yusuf M
(2017)
Procedures for cryogenic X-ray ptychographic imaging of biological samples.
in IUCrJ
| Description | We coordinated with a team of Japanese colleagues interested in participating in a 2-day conference. We decided to attach to the International Chromosome Conference in Bankok, Thailand as a satellite meeting. A full program was developed and followed. Travel expenses for 6 speakers were paid from the BBSRC budget. |
| Exploitation Route | Meeting was announced in public and attendance will be open. By attaching as a satellite to another meeting, we had hoped to increase impact. This was successful as we had an audience of about 70 persons, with many locals from the nascent Thai community. |
| Sectors | Agriculture, Food and Drink,Education,Environment,Healthcare,Culture, Heritage, Museums and Collections,Pharmaceuticals and Medical Biotechnology,Other |
| Description | The final scientific session was chaired by Dr Shinichi Ogawa: Prof. Susumu Uchiyama spoke about "Construction of cell lines for metaphase chromosome ChIP using CRISPR/Cas9 system", then Prof. Kiichi Fukui gave a Closing Speech. Prof. Ian Robinson and Prof. Kiichi Fukui then conducted a discussion for future grant applications and SACLA beamtime. Tow collaborative Human Frontiers gransts were then submitted, but neith was successful in attracting further funding. The beamtime application to |
| Organisation | Osaka University |
| Country | Japan |
| Sector | Academic/University |
| PI Contribution | Grant BB/L026260/1 was used to fund travel and accomodation for the 2nd UK-Japan Chromosome Workshop, held on 1st May 2015 in Kasetsart University, Bangkok, Thailand. It was a satellite meeting of the Asian Chromosome Conference (ACC) held the same week from April 29 to May 1, 2015 at Kasetsart University, Bangkok, Thailand. The Programme started on Friday 1st May 2015 with Prof. Ian Robinson giving a Welcome Speech and Dr Kornsorn Srikulnath providing an "Introduction to Kasetsart University". The first scientific session on X-RAY IMAGING was chaired by Prof. Susumu Uchiyama: Prof. Yoshinori Nishino spoke about "Chromosome structure as Revealed by X-ray Scattering", then Dr Mohammed Yusuf spoke about "Chromosome sample preparation for X-ray Imaging", then Dr Joerg Schwenke spoke about "Quantitative phase imaging of human chromosomes and nuclei using X-ray diffraction", then Dr Bo Chen spoke about "A Brief Comparison of SEM and TXM Imaging of Human Chromosomes". The second scientific session on FLUORESCENCE MICROSCOPY was chaired by Prof. Stanley Botchway: Prof. Hans de Jong spoke about "Advanced high-resolution microscopy status and prospects. An Overview", then Dr Naoki Wada spoke about "A hybrid cell line between human and plant", then Ms Gina Ratnasari spoke about "Single chromatid chromosome preparation using RNAi", then Ms Rinyaporn Pengchat spoke about "The effect of calcium ion on chromosome structure". The third scientific session on HIGH RESOLUTION - FLUORESCENCE MICROSCOPY was chaired by Prof. Hans de Jong: Prof. Stanley Botchway spoke about "New fluorescent probes for chromosome imaging: FLIM-PLIM and beyond", then Mr Christophe Lynch spoke about "Super Resolution imaging of chromosomes", then Ms Rawin Poonperm spoke about "Study of KIF4 localization in mitotic chromosomes", then Ms Ana Estandarte spoke about "FLIM Imaging of chromosomes". The fourth scientific session on ELECTRON MICROSCOPY was chaired by Prof. Yoshinori Nishino: Dr Shinichi Ogawa spoke about "He microscope for imaging of soft materials such as chromosome", then Mr Teruo Hashimoto spoke about "3D Serial Block face imaging preparation and applications", then Mr Kohei Kaneyoshi spoke about "Inner chromosome structure observed by FIB/SEM". |
| Collaborator Contribution | Grant BB/L026260/1 was used to fund travel and accomodation for the 2nd UK-Japan Chromosome Workshop, held on 1st May 2015 in Kasetsart University, Bangkok, Thailand. It was a satellite meeting of the Asian Chromosome Conference (ACC) held the same week from April 29 to May 1, 2015 at Kasetsart University, Bangkok, Thailand. The Programme started on Friday 1st May 2015 with Prof. Ian Robinson giving a Welcome Speech and Dr Kornsorn Srikulnath providing an "Introduction to Kasetsart University". The first scientific session on X-RAY IMAGING was chaired by Prof. Susumu Uchiyama: Prof. Yoshinori Nishino spoke about "Chromosome structure as Revealed by X-ray Scattering", then Dr Mohammed Yusuf spoke about "Chromosome sample preparation for X-ray Imaging", then Dr Joerg Schwenke spoke about "Quantitative phase imaging of human chromosomes and nuclei using X-ray diffraction", then Dr Bo Chen spoke about "A Brief Comparison of SEM and TXM Imaging of Human Chromosomes". The second scientific session on FLUORESCENCE MICROSCOPY was chaired by Prof. Stanley Botchway: Prof. Hans de Jong spoke about "Advanced high-resolution microscopy status and prospects. An Overview", then Dr Naoki Wada spoke about "A hybrid cell line between human and plant", then Ms Gina Ratnasari spoke about "Single chromatid chromosome preparation using RNAi", then Ms Rinyaporn Pengchat spoke about "The effect of calcium ion on chromosome structure". The third scientific session on HIGH RESOLUTION - FLUORESCENCE MICROSCOPY was chaired by Prof. Hans de Jong: Prof. Stanley Botchway spoke about "New fluorescent probes for chromosome imaging: FLIM-PLIM and beyond", then Mr Christophe Lynch spoke about "Super Resolution imaging of chromosomes", then Ms Rawin Poonperm spoke about "Study of KIF4 localization in mitotic chromosomes", then Ms Ana Estandarte spoke about "FLIM Imaging of chromosomes". The fourth scientific session on ELECTRON MICROSCOPY was chaired by Prof. Yoshinori Nishino: Dr Shinichi Ogawa spoke about "He microscope for imaging of soft materials such as chromosome", then Mr Teruo Hashimoto spoke about "3D Serial Block face imaging preparation and applications", then Mr Kohei Kaneyoshi spoke about "Inner chromosome structure observed by FIB/SEM". |
| Impact | Damage-free imaging of human chromosomes Ian Robinson, Mohammed Yusuf, Joerg Schwenke, Ana Estandarte, Fucai Zhang, Gurdeep Bhella, Neha Parmar, Jesse Clark, Changyong Song, Daewoong Nam, Gina Ratnasari, Kohei Kaneyoshi, Hideaki Takata and Kiichi Fukui, Chromosome Science 18 69-72 (2016) Towards Single Particle Imaging of human chromosomes at SACLA Ian Robinson, Joerg Schwenke, Mohammed Yusuf, Ana Estandarte, Fucai Zhang, Bo Chen, Jesse Clark, Changyong Song, Daewoong Nam, Yasumasa Joti, Kensuke Tono, Makina Yabashi, Gina Ratnasari, Kohei Kaneyoshi, Hideaki Takata and Kiichi Fukui, Journal of Physics B: Atomic, Molecular and Optical Physics, 48 244007 (2015) |
| Start Year | 2015 |
| Description | UK-Japan Chromosome workshop at ACC5 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Policymakers/politicians |
| Results and Impact | HIGH$RESOLUTION$-$FLUORESCENCE$MICROSCOPY$ Session$chair$Prof.$Hans$de$Jong$ $ 1.30! Prof.&Stanley&Botchway& New&fluorescent?obes&for&chromosome&imaging:&FLIM?& PLIM?&beyond& 2.00! Mr&Christophe&Lynch& SuperRsolution&imaging&of&chromosomes& 2.15$ Ms&Rawin&Poonperm& Study&of&KIF4&localization?&mitotic&chromosomes& 2.30$ Ms&Ana&Estandarte& FLIMIaging&of&chromosomes& 2.45$-$3.05$BREAK$! ELECTRON$MICROSCOPY$ Session$chair$Prof.$Yoshinori$Nishino$ 3.05! Dr&Shinichi&Ogawa& for&imaging&of&soft&materials&such&as&chromosome?& 3.35! Mr&Teruo&Hashimoto&& 3D&Serial&Block&face&imaging?paration?& applications& 3.55! Mr&Kohei&Kaneyoshi&& Inner&chromosome&structure&observed&by&FIB/SEM&& $WORKSHOP$END$PRESENTATION$$ Session$chair$Dr$Shinichi$Ogawa! 4.10$ Prof.&Susumu&Uchiyama& Construction&of&cell&lines&for&metaphase&chromosome& ChIP&using&CRISPR/Cas9&&system&& 4.40$ Prof.&Kiichi&Fukui& Closing&Speech& $4.45$-$5.10$BREAK! 5.10$- 6.00! Prof.&Ian&Robinson/&& Prof.&Kiichi&Fukui&& & All&welcome! Discussion&for&future&grant˜plications?&SACLA& beamtime& 7.00$pm$onwards$-$Evening$meal$$ $ Pola$Pola$Restaurant$and$Bar$$ End$of$meeting$ 2ndUK-Japan Chromosome Workshop, 1st May 2015 Venue: Kasetsart UniversityBangkok, Thailand Programme$ Friday$1st$May$2015! 9.00! Prof.&Ian&Robinson! Welcome&Speech& 9.05! Dr&Kornsorn&Srikulnath& Introduction&to&Kasetsart&University& X$RAY$IMAGING$$ Session$chair$Prof.$Susumu$Uchiyama$ 9.15! Prof.&Yoshinori&Nishino&! Chromosome&structure&asRvealed&by&X?ray& Scattering&! 9.45! Dr&Mohammed&Yusuf&! Chromosome&sample?paration&for&X?rayIaging! 10.05! Dr&Joerg?hwenke! Quantitative&phase&imaging&of&human&chromosomes& and?clei&using&X?ray&diffraction! 10.25! Dr&Bo&Chen! A&Brief&Comparison&of&SEM?&TXMIaging&of& Human&Chromosomes& 10.45$-$11.05$BREAK! FLUORESCENCE$MICROSCOPY! Session$chair$Prof.$Stanley$Botchway! 11.05! Prof.&Hans&de&Jong& Advanced&high&?&resolutionµscopy&?&status?& prospects.&An&Overview& 11.35! Dr&Naoki&Wada& A&hybrid&cell&line?&human?&plant&& 11.55! Ms&Gina&Ratnasari&& Single&chromatid&chromosome?paration&using&RNAi& 12.10$ Ms&Rinyaporn&Pengchat& The&effect&of&calcium&ion&on&chromosome&structure& $12.25$-$01.30$LUNCH$! |
| Year(s) Of Engagement Activity | 2016 |
| URL | https://acc5bangkok2015.kasetsart.org/acc5/ |