Translation of a novel electrical stimulation perfusion bioreactor
Lead Research Organisation:
University of Manchester
Department Name: Materials
Abstract
This project focuses on translating an existing bioreactor design that can be used to grow a variety of tissues in the laboratory. In particular, we will be optimising the growth of cardiac muscle and neural tissue in a 3D perfusion bioreactor whilst incorporating electrical stimulation regimes. The bioreactor configuration is entirely novel and we will strengthen and finalise our existing IP and file a patent as a result of this grant. This project will engage with a variety of industrial collaborators in market steering capacity and tissue phenotype maintenance proof of concept data will be gained.
Publications
Cartmell S.H.
(2016)
Electrical stimulation: a tool for tissue engineers
Cartmell SH
(2016)
Electrical stimulation: a tool for tissue engineering
in European Cells and Materials
Mobini S
Changes in human mesenchymal stem cells behaviors in low-voltage direct current electrical field
in European Cells and Materials
Mobini S
(2016)
Effect of direct current electrical stimulation on cell morphology, proliferation, and osteogenic differentiation of human mesenchymal stem cells
in European Cells and Materials
Mobini S
(2017)
Electrical Stimulation Changes Human Mesenchymal Stem Cells Orientation and Cytoskeleton Organization
in Journal of Biomaterials and Tissue Engineering
Mobini S., Srirussamee K., Xue R., Talts Ü., Balint R., Cassidy N.J., Cartmell S.H.
(2016)
Changes in human mesenchymal stem cells behaviours in low-voltage direct current electrical field
| Description | Development of perfusion bioreactor with electrical stimulus - tested in collaboration with Loughborough University for skeletal muscle Development of electrical stimulator bioreactor for bone tissue engineering. Furthered understanding regarding mechanism of cell response including role of faraday byproducts and heterogeneity of electrical field in different bioreactor systems. Furthered understanding of cathode local cell responses in terms of cell alignment and maturation. |
| Exploitation Route | A super follow on fund from BBSRC is currently being applied for (An EOI has already been successful in March 2016). This grant will include input from pharma companies and other stakeholders for development of 3D tissue culture for bone, tendon and skeletal muscle for toxicology applications and ultimately tissue engineering strategies. |
| Sectors | Electronics,Healthcare |
| Description | National and international collaborations have been established with academics, industry and clinicians as a result of this grant funding. An EOI for BBSRC super follow on funding has been submitted and was successful in March 2016. Full sFOF planned for submission in October 2017. |
| First Year Of Impact | 2016 |
| Sector | Healthcare,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Societal |
| Description | Prof Sarah Waters |
| Organisation | University of Oxford |
| Department | Mathematical Institute Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | New contribution |
| Collaborator Contribution | New contribution |
| Impact | New contribution |
| Start Year | 2015 |
| Description | Prof. Albrecht Molsberger |
| Organisation | Heinrich Heine University Düsseldorf |
| Department | Department of Orthopaedies |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | New contribution |
| Collaborator Contribution | New contribution |
| Impact | New contribution |
| Start Year | 2015 |
| Description | Conference Management Committee and seminar presentation |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Cartmell, SH; Management and Chairing "Electrical Stimulation in Tissue Engineering and Regenerative Medicine Symposium" at EU-TERMIS, June 2016, Uppsala, Sweden. |
| Year(s) Of Engagement Activity | 2016 |
| Description | Conference presentation (EU-TERMIS 2016) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Effect of direct current electrical stimulation on cell morphology, proliferation, and osteogenic differentiation of human mesenchymal stem cells |
| Year(s) Of Engagement Activity | 2013,2016 |
| Description | Conference symposium (EU-TERMIS 2016) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Electrical stimulation: a tool for tissue engineers |
| Year(s) Of Engagement Activity | 2016 |
| Description | Oral and Poster presentation at International Conference- EU-TERMIS 2016 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | 1 Oral keynote talk and 1 poster at EU-TERMIS Uppsala- June 2016 were presented. |
| Year(s) Of Engagement Activity | 2016 |
| Description | Oral and Poster presentation at National Conference- TCES 2016 |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | 2 posters and 1 oral talk at TCES London- July 2016 |
| Year(s) Of Engagement Activity | 2015,2016 |
| Description | Training Course presentation |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Mobini S, "Electrical stimulation of tissues". A training lecture presented at Bioreactor & Growth Environments for Tissue Engineering Training Course in Keel (United Kingdom) in November 2015. |
| Year(s) Of Engagement Activity | 2015 |