Glycoenzymes for Bioindustries
Lead Research Organisation:
John Innes Centre
Department Name: Biological Chemistry
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
The project will translate existing academic work on the development of novel glycoenzymes to produce a suite of stable/robust enzymes at scale to address the current gaps in certain commercially available specificities. The lack of availability of such enzymes is a major barrier to those wishing to utilise them for diagnostics, synthesis or chemical modification processes often necessitating de novo synthesis of the enzyme targeting the required reaction before development/evaluation of the process can begin. Each academic partner will develop a subset of the glycoenzyme classes, providing the necessary analytical expertise to characterise and optimise the enzymes. Underpinning this work will be genome mining combined with enzyme evolution to discover/develop novel microbial sourced enzymes displaying desired activities. Industry input to the project will guide the development of the enzymes whilst ensuring that they are fit for purpose and brought to market to allow end user access.
People |
ORCID iD |
Rob Field (Principal Investigator) |
Publications
Bulmer GS
(2021)
Recent advances in enzymatic synthesis of ß-glucan and cellulose.
in Carbohydrate research
Gabrielli V
(2021)
Molecular Recognition of Natural and Non-Natural Substrates by Cellodextrin Phosphorylase from Ruminiclostridium Thermocellum Investigated by NMR Spectroscopy
in Chemistry - A European Journal
Kuhaudomlarp S
(2019)
Unravelling the Specificity of Laminaribiose Phosphorylase from Paenibacillus sp. YM-1 towards Donor Substrates Glucose/Mannose 1-Phosphate by Using X-ray Crystallography and Saturation Transfer Difference NMR Spectroscopy.
in Chembiochem : a European journal of chemical biology
Kuhaudomlarp S
(2018)
Identification of Euglena gracilis ß-1,3-glucan phosphorylase and establishment of a new glycoside hydrolase (GH) family GH149
in Journal of Biological Chemistry
Monestier M
(2017)
Membrane-enclosed multienzyme (MEME) synthesis of 2,7-anhydro-sialic acid derivatives.
in Carbohydrate research
Panpetch P
(2018)
Cloning of the full-length isoamylase3 gene from cassava Manihot esculenta Crantz 'KU50' and its heterologous expression in E. coli.
in Plant physiology and biochemistry : PPB
Panpetch P
(2018)
Heterologous co-expression in E. coli of isoamylase genes from cassava Manihot esculenta Crantz 'KU50' achieves enzyme-active heteromeric complex formation.
in Plant molecular biology
Pergolizzi G
(2016)
Carbohydrate Chemistry - Volume 42
Wangpaiboon K
(2018)
An a-1,6-and a-1,3-linked glucan produced by Leuconostoc citreum ABK-1 alternansucrase with nanoparticle and film-forming properties.
in Scientific reports
Description | New carbohydrate-active enzymes identified - phosphorylases for b-1,3-glucosylation and b-1,4-glucosylation, both of which will also transfer galactose, glucosamine and mannose regio- and stereo-specifically. |
Exploitation Route | Incorporate enzyme into scalable synthesis process progressing well. New enzymatic routes to cellulose piolysaccharide materials effective, identfying a new polymorph of cellulose. |
Sectors | Agriculture, Food and Drink,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
Description | New engagement with Unilever, who have broad-ranging interest in gaining insight into how to design and exploit polysaccharide materials. |
First Year Of Impact | 2019 |
Sector | Agriculture, Food and Drink,Chemicals,Environment,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
Impact Types | Societal,Economic |
Title | Enzyme to prepare beta-glucans |
Description | Identification of key enzyme and establishment of SOP for the enzymatic synthesis of immune-active beta-glucans. |
Type Of Material | Technology assay or reagent |
Year Produced | 2019 |
Provided To Others? | Yes |
Impact | Too early to tell |
Description | CDs |
Organisation | Newcastle University |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Evaluation of a glycosynthase for novel cyclodextrin production |
Collaborator Contribution | Provision of clone of wild type enzyme and active site mutant |
Impact | Too early to judge |
Start Year | 2017 |
Description | Human milk oligosaccharides |
Organisation | DSM |
Department | DSM Biotechnology Centre |
Country | Netherlands |
Sector | Private |
PI Contribution | Know-how and expertise for the chemical modification of carbohydrates. |
Collaborator Contribution | Provision of 100mg-1g quantities of 6 human milk oligosaccharides. |
Impact | Only just started |
Start Year | 2021 |
Description | PPase |
Organisation | University of York |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Work up of scalable synthesis of N-glycan analogues |
Collaborator Contribution | Clone of N-glycan phosphorylase |
Impact | Too early |
Start Year | 2017 |
Description | Sugar nucleotides |
Organisation | Keele University |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Enzymatic synthsesis |
Collaborator Contribution | Chemical synthesis - methods development and target synthesis. |
Impact | New integrated chemical and enzymatic methods for sugar nucleotide synthesis |
Start Year | 2020 |