Pilot Algal Lipid Manufacturing in the United Kingdom (PALM-UK)
Lead Research Organisation:
Rothamsted Research
Department Name: Plant Sciences
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Planned Impact
As described in proposal submitted to IUK
Publications
Degraeve-Guilbault C
(2017)
Glycerolipid Characterization and Nutrient Deprivation-Associated Changes in the Green Picoalga Ostreococcus tauri.
in Plant physiology
Dolch LJ
(2017)
A Palmitic Acid Elongase Affects Eicosapentaenoic Acid and Plastidial Monogalactosyldiacylglycerol Levels in Nannochloropsis.
in Plant physiology
Hamilton ML
(2016)
Heterotrophic Production of Omega-3 Long-Chain Polyunsaturated Fatty Acids by Trophically Converted Marine Diatom Phaeodactylum tricornutum.
in Marine drugs
Haslam RP
(2016)
Synthetic redesign of plant lipid metabolism.
in The Plant journal : for cell and molecular biology
| Description | We have developed multifunctionilized algal strains able to produce high value lipids and proteins. We have engineered the marine diatom Phaeodactylum tricornutum to accumulate high levels of omega-3 EPA and DHA together with recombinant proteins, the fungal or bacterial phytases. |
| Exploitation Route | We demonstrated the feasibility of developing nultifunctionalized microalgae for the production of industrial useful proteins and fatty acids to meet the demand of modern intensive fish farming industry. Further animal studies will evaluate the suitability of generated strains for use within commercial feed applications |
| Sectors | Agriculture, Food and Drink,Chemicals,Energy,Environment,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| Description | Grant review |
| Geographic Reach | Europe |
| Policy Influence Type | Membership of a guideline committee |
| Description | Member of advisory committee |
| Geographic Reach | Europe |
| Policy Influence Type | Participation in a guidance/advisory committee |
| Description | High Value Chemicals from Plants network |
| Amount | £47,289 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2016 |
| End | 09/2016 |
| Description | Newton fund |
| Amount | £10,000 (GBP) |
| Organisation | British Council |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 04/2016 |
| End | 04/2017 |
| Description | UK-China Collaboration to tackle Antimicrobial Resistance |
| Amount | £783,000 (GBP) |
| Funding ID | 104981 |
| Organisation | Innovate UK |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2019 |
| End | 02/2022 |
| Title | Chloroplast transformation |
| Description | Development of chloroplast transformation tool kit for marine diatoms |
| Type Of Material | Biological samples |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | The developed method of transformation will facilitate the expression of foreign proteins in algal chloroplas |
| Title | Transgenic microalgae expressing multiple peptides |
| Description | Generation of transgenic diatom accumulating target peptides or proteins and specific enzymes |
| Type Of Material | Cell line |
| Provided To Others? | No |
| Impact | Multifunctional modification of algal strains |
| Description | Characterization of Ostreococcus tauti glycerolipidome |
| Organisation | University of Bordeaux |
| Department | Laboratory of Membrane Biology |
| Country | France |
| Sector | Academic/University |
| PI Contribution | Acyl-CoA analysis of O.tauri strain |
| Collaborator Contribution | Analysis of fatty acid distribution and partitioning into different lipid classes |
| Impact | From a biotechnological point of view, unravelling the mechanisms involved in the synthesis of TAGs species containing highly valuable FAs such as ALA, SDA and DHA might have important practical outcome |
| Start Year | 2016 |
| Description | Enhancing PUFA production in Nannochloropsis |
| Organisation | University of Grenoble |
| Country | France |
| Sector | Academic/University |
| PI Contribution | Functional characterization of palmitic acid elongase in yeast |
| Collaborator Contribution | Eight fatty acid elongases and six desaturases involved in VLC-PUFA production in Nannochloropsis gaditana have been identified. The highly expressed isoform ?0-ELO1 has been characterized via targeted gene knock-out (KO). The 0-elo1 mutants had a reduced EPA level and displayed a functionally impaired photosynthesis. |
| Impact | This study gives insights on the regulation of lipid synthesis in heterokonts and also provides interesting prospects for the engineering of these algae for biotechnological applications. |
| Start Year | 2016 |
| Description | Glycerolipid profiling of P.tricornutum ACP-d9 desaturase overexpressers |
| Organisation | European Molecular Biology Laboratory |
| Department | EMBL-Grenoble |
| Country | France |
| Sector | Academic/University |
| PI Contribution | Generation of transgenic diatom overexpressing genes of LC-PUFA biosynthesis |
| Collaborator Contribution | Glycerolipid analysis including quantification of lipid species |
| Impact | Generation of improved algal strains for protein expression and lipid accumulation |
| Start Year | 2014 |
| Description | Increasing C-flux towards the synthesis of acetyl-CoA |
| Organisation | University of Huelva |
| Country | Spain |
| Sector | Academic/University |
| PI Contribution | Study on FA and neutral lipids accumulation in N-starved cultures of Chlamydomonas reinhardtii |
| Collaborator Contribution | Analysis of the acetyl-CoA metabolism |
| Impact | Development of an alternative strategy to boost lipid biosynthesis in microalgae |
| Start Year | 2016 |
| Title | TRANSGENIC MICROALGAE WITH INCREASED PRODUCTION OF AT LEAST ONE OMEGA-3 LONG CHAIN POLYUNSATURATED FATTY ACID |
| Description | The invention relates to genetically modified organisms with enhanced production of omega-3 long chain polyunsaturated fatty acids. |
| IP Reference | US2018312888 |
| Protection | Patent application published |
| Year Protection Granted | 2018 |
| Licensed | No |
| Impact | Exclusive use of this transgenic strain as a platform to produce additional high value molecules including lipids and proteins |
| Description | Conference presentation |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | ABBA conference, Seattle. Future collaboration with industrial partners have been discussed. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Keynote lecture |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Keynote lecture at the Congress of European Federation of Biotechnology, Olomouc, June, 2017. 300 people attended and the lecture was filmed for further use for educational purposes |
| Year(s) Of Engagement Activity | 2010,2017 |
| Description | Keynote lecture |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | Bordeaux, Conference on Lipids in Cosmetics. Multiple discussions with business representatives |
| Year(s) Of Engagement Activity | 2018 |
| Description | Keynote lecture |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Industry/Business |
| Results and Impact | 15th Euro Fed Lipid Congress, Uppsala. The lecture was followed by discussions afterwards bringing together representatives from Industries and Academia. Several potential projects have been discussed. |
| Year(s) Of Engagement Activity | 2017 |
| Description | RRES seminar |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | ~100 scientists from RRES attended the talk which sparked questions and discussion on the future of marine biotechnology |
| Year(s) Of Engagement Activity | 2016 |
| Description | Sustainable aquaculture meeting (London) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | Over 100 scientists and industry representatives attended the conference dedicated to the development of sustainable aquaculture for food and feed |
| Year(s) Of Engagement Activity | 2016 |
| Description | students' meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | 100 students attended the workshop on "new platforms for the production of high value molecules" |
| Year(s) Of Engagement Activity | 2017 |