Cuba: Application of 'omics in the metabolic study of high cell density continuous cell cultures of recombinant NS0 myeloma cell lines
Lead Research Organisation:
University of Manchester
Department Name: Chem Eng and Analytical Science
Abstract
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People |
ORCID iD |
| Alan Dickson (Principal Investigator) |
Publications
Latorre Y
(2023)
Engineering of Chinese hamster ovary cells for co-overexpressing MYC and XBP1s increased cell proliferation and recombinant EPO production.
in Scientific reports
Torres M
(2021)
Metabolic profiling of Chinese hamster ovary cell cultures at different working volumes and agitation speeds using spin tube reactors.
in Biotechnology progress
Torres M
(2018)
Process and metabolic engineering perspectives of lactate production in mammalian cell cultures
in Current Opinion in Chemical Engineering
| Description | The application set out 5 objectives: (1) To build understanding of the technology needs of the Centre of Molecular Immunology (CIM), Havana, for enhancement of manufacture of recombinant proteins in high density continuous cell culture processes of NS0 myeloma cell lines This has been achieved via the exchange activities and collaborative activities developed during the early stages of the project. This has enabled us to bring together UoM researchers with appropriate CIM researchers and these groupings are now making their own applications for international funding (2) To translate knowledge from the CIM into research projects of high density continuous cell culture processes being undertaken in the Dickson laboratory with the industry-standard, Chinese Hamster Ovary cell platform This has been achieved, primarily via the research visit of Dr Morales, as a consequence the Dickson lab have developed in-house media that support enhanced high cell density CHO cell growth. These media are now being tested with a set of cell lines. (3) To build a trans-national research partnership to leverage future grant applications/funding, bringing together the technologies and facilities at the University of Manchester and the knowledge built up at the CIM to enhance manufacturing of new biological medicines This has in part been achieved with the most productive areas likely to be linked to the work of Drs Curtis and Swanton. The uncertainty with respect to EU sector grant applications has restricted the potential to maximise on the groundwork undertaken to date. (4) To develop the potential for wider research collaborations between The University of Manchester and the CIM We have signed a MoU with CIM and further fully-funded visits of UoM staff to CIM are due to occur in November of this year. (5) To engage the research strengths and activities of the CIM in Cuba to the UK biopharmaceuticals sector via the BioProNET Network in Industrial Biotechnology Dr Hernandez attended two BioProNET annual meetings and, as well as presenting on one occasion, she met with the leaders of the UK sector and engaged in discussions that have continued outside the context of this project. |
| Exploitation Route | Prior to our engagement with CIM there has been very little engagement between the UK biologics sector and the related Cuban sector. In the short time frame of this BBSRC grant, we have provided an inroad to, and have developed good working relationships with, the senior managers and researchers at CIM, whilst introducing these same individuals (and the capabilities in CIM and Cuba, generally) to the UK commercial and academic bodies. Our (UoM) personal intentions are to develop researcher exchange grant applications with CIM (potentially in partnership with other Latin and South American groups) when appropriate calls appear in GCRF schemes. Our interactions have shown the strong research acumen of many of the early career Cuban researchers. We are encouraging those individuals to make application to appropriate Fellowship schemes. The results we have had to date arose form a relatively static state and the relationship built up as a consequence of BBSRC funding will allow us to maintain the interactions whilst applying for larger funding streams to generate a greater outcome. |
| Sectors | Agriculture, Food and Drink,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| Description | EMBO Fellowship |
| Amount | £7,500 (GBP) |
| Organisation | European Molecular Biology Organisation |
| Sector | Charity/Non Profit |
| Country | Germany |
| Start | 03/2017 |
| End | 06/2017 |
| Description | University of Manchester Research Institute funding scheme |
| Amount | £19,000 (GBP) |
| Organisation | University of Manchester |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 05/2016 |
| End | 07/2017 |
| Description | University of Manchester/FAPESP award |
| Amount | £16,000 (GBP) |
| Organisation | São Paulo Research Foundation (FAPESP) |
| Sector | Public |
| Country | Brazil |
| Start | 01/2018 |
| End | 12/2019 |
| Description | Chile |
| Organisation | Pontifical Catholic University of Valparaiso |
| Country | Chile |
| Sector | Academic/University |
| PI Contribution | Examining common interests in CHO cell metabolism. Manchester contributing to metabolomics assessments of engineered CHO cell lines. Chile (Prof Claudia Altamirano) contributing PhD studentship providing support and technical expertise to undertake experimental work. |
| Collaborator Contribution | Introductions to Prof Altamirano were by Dr Kathya Hernandez (CIM). The Chilean input has supplied technical input, funds and in-kind provision of CHO cell lines. |
| Impact | Publication already generated: Torres et al (2019) |
| Start Year | 2017 |
| Description | Research Collaboration between University of Manchester and Centre for Molecular Immunology, Havana |
| Organisation | Centre of Molecular Immunology |
| Country | Cuba |
| Sector | Private |
| PI Contribution | Dickson and other academic members of the Centre of Excellence in Biopharmaceuticals have specific technical and intellectual skills that offer collaborative opportunities for working with the Centre for Molecular Immunology (CIM), especially in the areas of enhanced cell culture performance, analytical assessment of product quality and novel approaches to formulation. Meetings (held in the UK and Havana) have generated the development of a formal academic collaboration agreement. Projects will be financed via multiple routes. |
| Collaborator Contribution | The Research Director at CIM and several of the research scientists have direct involvement in specific projects that are just initiating. Staff exchange models have started with an EMBO Research Fellowship supporting the exchange visit of Orlando Morales to the Dickson lab for 3 months in mid-2017. This formalised the potential for success in the collaborations and has provided the format for further collaborative activity. |
| Impact | None yet. Multi-disciplinary - cell and molecular biology, analytical sciences, biophysics, chemical engineering, pharmacy, immunology |
| Start Year | 2016 |