Exploiting phytase variants from soils as a source of enzymes to improve animal feeds
Lead Research Organisation:
Rothamsted Research
Department Name: Sustainable Soils and Grassland Systems
Abstract
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People |
ORCID iD |
| Andrew Neal (Principal Investigator) |
Publications
| Description | Beta-propeller phytases are not of any significant industrial interest because of their limited pH range. However, other classes of phytase found in soil may be of more interest and these are being studied more closely now. We have identified a second family of phosphohydrolases - multiple inositol-polyphosphate phosphatases (MINPP) - with soil phytase activity, and identified Acinetobacter spp. as harbouring this prevalent soil phytase activity. Previously, bacterial MINPP activity was described only in gut commensals. |
| Exploitation Route | This report can be used by others to disqualify beta-propeller phytase variants as potential sources of biotechnological products. Other phytase variants such as Histidine Acid and Cysteine Phytases hold more potential |
| Sectors | Agriculture, Food and Drink,Healthcare,Manufacturing, including Industrial Biotechology |
| Title | pHMM models |
| Description | development of profile hidden Markov models for each of genes coding for acid and alkaline phosphatses and phytases - 9 in total |
| Type Of Material | Biological samples |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | improved detection of phosphatase and phytase genes in soil metagenomes will aid discovery of new environmental variants with potential industrial applications |
| Description | phytase activiety in soil isolates |
| Organisation | University of East Anglia |
| Department | School of Biological Sciences UEA |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | I have contributed expertise with respect to metagenome-guided isolation of soil bacteria with potential phytase activity, intellectual input into future collaborative experimental work and training of a PhD student with respect to the isolation and culture of soil microbes |
| Collaborator Contribution | Partners have contributed expertise in phytate chemistry and enzymmatic assays as well as HPLC detection of enzyme mechanisms |
| Impact | No outcomes to date. Collaboration is multi-disciplinary, including microbiology, chemistry and enzymology |
| Start Year | 2017 |