Loving the poison: molecular basis for metabolism of the widely-used food preservative, propionate in Pseudomonas aeruginosa.
Lead Research Organisation:
University of Cambridge
Department Name: Biochemistry
Abstract
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People |
ORCID iD |
| Martin Welch (Principal Investigator) |
Publications
Dolan SK
(2018)
The Glyoxylate Shunt, 60 Years On.
in Annual review of microbiology
Dolan SK
(2020)
Transcriptional regulation of central carbon metabolism in Pseudomonas aeruginosa.
in Microbial biotechnology
Dolan SK
(2018)
Loving the poison: the methylcitrate cycle and bacterial pathogenesis.
in Microbiology (Reading, England)
| Description | We have identified (through FBDD approaches, with back-to-back XChem grants) inhibitors for the PrpC and PrpB proteins. This is significant because inhibition of PrpC/PrpB causes lethality in the presence of propionate, which is abundant in infection scenarios. This is therefore a route towards antibacterial development, and we are working with this in mind. |
| Exploitation Route | We are in discussion with LifeArc about the best way to move this forward. We also received BBSRC IAA funding to do the same. |
| Sectors | Pharmaceuticals and Medical Biotechnology |
| Description | LB19242 2-METHYLISOCITRATE LYASE AS A NOVEL ANTIBACTERIAL AGENT. |
| Amount | £35,000 (GBP) |
| Organisation | Diamond Light Source |
| Sector | Private |
| Country | United Kingdom |
| Start | 10/2017 |
| End | 09/2019 |
| Description | LB21553 TARGETING METHYLISOCITRATE LYASE (PRPB) IN THE HUMAN PATHOGEN, PSEUDOMONAS AERUGINOSA. |
| Amount | £35,000 (GBP) |
| Organisation | Diamond Light Source |
| Sector | Private |
| Country | United Kingdom |
| Start | 09/2019 |
| End | 10/2020 |
| Title | in silico systems approaches to transcriptional networks |
| Description | My PDRA visited Brazil (Silva-Rocha lab) to learn how to carry out network analyses of his transcriptome data |
| Type Of Material | Model of mechanisms or symptoms - in vitro |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | 1 paper published, 1 paper invited from publisher, 1-2 papers in preparation (to be published 2018 sometime) |
| Description | Strengthening of collaborations |
| Organisation | University of Cambridge |
| Department | Cambridge Centre for Proteomics |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Continuation (from 12-05-2008 to the present day) and strengthening of my ongoing collaborative partnership with Dr David Spring in the Chemistry Dept and Dr Kathryn Lilley (co-applicant) in the Cambridge Centre for Proteomics research. Both ongoing collaborations continue to be active and productive. |
| Start Year | 2008 |
| Description | analysis of microarray data |
| Organisation | Quadram Institute Bioscience |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | IFR assisted us in the analysis of some microarray data |
| Collaborator Contribution | microarray data analysis |
| Impact | Bowden et al Ml Micro paper (see publications arising) |
| Start Year | 2012 |