Japan Partnering : Damage free structures of enzymes of denitrification pathway and their complexes using SF-ROX and SFX at SACLA XFEL
Lead Research Organisation:
University of Liverpool
Department Name: Institute of Integrative Biology
Abstract
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People |
ORCID iD |
| Samar Hasnain (Principal Investigator) |
Publications
Eady R
(2022)
New horizons in structure-function studies of copper nitrite reductase
in Coordination Chemistry Reviews
Gopalasingam CC
(2022)
Frontiers in metalloprotein crystallography and cryogenic electron microscopy.
in Current opinion in structural biology
Gopalasingam CC
(2019)
Dimeric structures of quinol-dependent nitric oxide reductases (qNORs) revealed by cryo-electron microscopy.
in Science advances
Hedison TM
(2019)
Unexpected Roles of a Tether Harboring a Tyrosine Gatekeeper Residue in Modular Nitrite Reductase Catalysis.
in ACS catalysis
Hedison TM
(2019)
Solvent-slaved protein motions accompany proton coupled electron transfer reactions catalysed by copper nitrite reductase.
in Chemical communications (Cambridge, England)
Jamali MAM
(2020)
The active form of quinol-dependent nitric oxide reductase from Neisseria meningitidis is a dimer.
in IUCrJ
Muench SP
(2019)
The expanding toolkit for structural biology: synchrotrons, X-ray lasers and cryoEM.
in IUCrJ
Rose SL
(2022)
Single crystal spectroscopy and multiple structures from one crystal (MSOX) define catalysis in copper nitrite reductases.
in Proceedings of the National Academy of Sciences of the United States of America
Rose SL
(2021)
An unprecedented insight into the catalytic mechanism of copper nitrite reductase from atomic-resolution and damage-free structures.
in Science advances
| Description | Copper-containing nitrite reductases (CuNiRs), encoded by nirK gene, are found in all kingdoms of life with only 5% of CuNiR denitrifiers having two or more copies of nirK. Recently, we have identified two copies of nirK genes in several a-proteobacteria of the order Rhizobiales including Bradyrhizobium sp. ORS 375, encoding a 4-domain heme-CuNiR and the usual 2-domain CuNiR (Br2DNiR). Compared with two of the best-studied 2-domain CuNiRs represented by the blue (AxNiR) and green (AcNiR) sub-classes, Br2DNiR, a blue CuNiR, shows a 20-fold lower catalytic efficiency despite a sequence identity of ~70%. Advanced synchrotron radiation (SR) and X-ray free electron laser (XFEL) are used to obtain most accurate (atomic resolution with unrestrained SHELX refinement) and damage-free (free from radiation-induced chemistry) structures, in as-isolated, substrate-bound and product-bound states. This combination has shed unprecedented light on the protonation states of essential catalytic residues, new reaction intermediates and how catalytic efficiency is modulated. This work was published in Science Advances (Rose et al., Sci. Adv. 2021; 7 : eabd8523). We recently combined on-line single crystal spectrorocopy with MSOX serial crystallography to report a step change in our understanding of Nitrite catalysis (reported in PNAS 2022 https://doi.org/10.1073/pnas.2205664119. |
| Exploitation Route | The idea of holding a UK-JAPAN sympsoium has been shelved till 2022 when we hope that the international travel would become feasible. |
| Sectors | Agriculture, Food and Drink,Environment,Manufacturing, including Industrial Biotechology |