Multi-User Cryo-Ultramicrotome for High Resolution and Immuno-Gold Analysis at UEA
Lead Research Organisation:
University of East Anglia
Department Name: Biological Sciences
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Publications
Goldspink DA
(2013)
The microtubule end-binding protein EB2 is a central regulator of microtubule reorganisation in apico-basal epithelial differentiation.
in Journal of cell science
Mogensen MM
(2007)
The deaf mouse mutant whirler suggests a role for whirlin in actin filament dynamics and stereocilia development.
in Cell motility and the cytoskeleton
Kohlmaier G
(2009)
Overly long centrioles and defective cell division upon excess of the SAS-4-related protein CPAP.
in Current biology : CB
Moss DK
(2007)
Ninein is released from the centrosome and moves bi-directionally along microtubules.
in Journal of cell science
Bellett G
(2009)
Microtubule plus-end and minus-end capture at adherens junctions is involved in the assembly of apico-basal arrays in polarised epithelial cells.
in Cell motility and the cytoskeleton
Description | The cryo-ultramicrotome obtained on the equipment grant was essential for much of the work carried out in my lab. Electron microscopy is an essential part of several of our published data. |
Exploitation Route | Other research groups on the NRP have used this equipment and still do |
Sectors | Agriculture, Food and Drink,Chemicals,Digital/Communication/Information Technologies (including Software),Education,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
Description | The REI awarded cryo-ultramicrotome has underpined key research supported by the BBSRC and others by enabling efficient ultra-thin sectioning for high resolution structural analyses and by providing a cryo-sectioning facility for immuno-electron microscope localisation of molecules vital for several studies in the School of Biological Sciences at UEA as well as at JIC. |
First Year Of Impact | 2006 |
Sector | Agriculture, Food and Drink,Digital/Communication/Information Technologies (including Software),Education,Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
Description | Advanced microscopy |
Organisation | University of East Anglia |
Department | School of Biological Sciences UEA |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Modification of some techniques, providing live samples for training and experiments |
Collaborator Contribution | Training, assistance and advise on various microscope techniques |
Impact | Training of postdoctoral researchers doi: 10.1242/jcs.129759 |
Description | The Clip1/2 knockout mouse |
Organisation | Erasmus University Rotterdam |
Country | Netherlands |
Sector | Academic/University |
PI Contribution | Provided detailed protocols for immuno-staining of isolated mouse tissue Discussed research findings |
Collaborator Contribution | Collaborator generated the Clip1/2 double knockout. Hosted and assisted postdoc with isolation and preparation of mouse intestine from wildtype and knockout mice Genotyped samples and organised shipping of mice Contributed to manuscript submitted |
Impact | http://dx.doi.org/10.1098/rsob.160274 |
Start Year | 2011 |
Description | The role of the SAS-4-related protein CPAP |
Organisation | Swiss Institute for Experimental Cancer Research (ISREC) |
Country | Switzerland |
Sector | Academic/University |
PI Contribution | Made a major contribution to this project providing extensive TEM analyses. I was a member of the supervisory team for the first author and I was also one of the external examiners for his PhD viva in Switzerland. |
Collaborator Contribution | Publication on which I was a co-author doi: 10.1016/j |
Impact | Training of postdoc Publication: doi: 10.1016/j |
Start Year | 2007 |
Description | Advances in stem cell biology ASGBI meeting |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | Yes |
Geographic Reach | National |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | PhD student presented research findings Training in poster presentation |
Year(s) Of Engagement Activity | 2007 |
Description | Bio colloquium |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | Yes |
Geographic Reach | Local |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Talks and poster presentations have lead to collaborations New collaborations |
Year(s) Of Engagement Activity | Pre-2006,2006,2007,2008,2009,2010,2011,2012,2013,2014 |
Description | British Society of Cell Biology meeting, Edinburgh (2009) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | Yes |
Geographic Reach | National |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | poster presentation Training in presentation and networking |
Year(s) Of Engagement Activity | 2009 |
Description | International conference on the Centrosome and Spindle Poles in Heidelberg, Germany |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | Yes |
Geographic Reach | International |
Primary Audience | Other academic audiences (collaborators, peers etc.) |
Results and Impact | Attended conference and gave a talk on our recent findings concerning the centrosome. Part of TEM work from my lab was also included on a poster by collaborator Work presented was subsequently published doi: 10.1002 doi: 10.1016/j |
Year(s) Of Engagement Activity | 2008 |