Energisation of nitrogen fixation in the Rhizobium-legume symbiosis
Lead Research Organisation:
John Innes Centre
Department Name: Molecular Microbiology
Abstract
Bacteria are simple single celled organisms that lack the membrane bound structures found in higher cells of plants and animals. However, while bacteria may have a less complex cellular organisation they carry out a huge range of chemical reactions not found in plants and animals. Bacteria are responsible for the cycling of many nutrients such as N2 (N2 is also known as nitrogen gas and consists of two nitrogen atoms bound by a strong triple bond), which is a very inert atmospheric gas. N2 makes up 78% of the atmosphere but is very unreactive and cannot be used directly as a source of nitrogen, which is needed for amino acid, protein and DNA synthesis. However, a small number of bacteria can reduce (add hydrogen) to N2 and convert it into ammonia (NH3), which is readily incorporated into amino acids and then all the other building blocks of life, by a wide range of organisms including bacteria and plants. In many parts of the world the limitation to growth of plants, which in turn support animal life, is the supply of nitrogen as ammonia or related compounds. Since up to 65% of available nitrogen (eg ammonia) comes from bacteria this makes them essential for life on earth. Within the bacteria, most of the nitrogen is actually produced by one family known as the Rhizobiacea. This remarkable group of bacteria form a symbiotic association (both partners benefit) with plants of the legume family, that results in the formation of root nodules (on pea plants these are 2-3 mm bulbs that can easily be seen by pulling up a plant and inspecting its roots). The rhizobia are held inside the nodules where the plant provides them with an ideal environment (low O2 and lots of energy) in which they can reduce N2 to ammonia. The ammonia is supplied to the plant as its nitrogen source so this is why this is known as a symbiotic interaction. It means that the plant does need any nitrogen in the soil and enables rapid growth. The purpose of this research is to understand the type of fuel provided by the plant to power the fixation of N2 to ammonia by the bacteria. Questions include how is the fuel delivered to the bacteria and how do they metabolise (break it down) it to simpler compounds. Finally, we want to know whether, apart from ammonia, the bacteria secrete other compounds to the plant.
Technical Summary
The Aap and Bra are broad specificity amino acid transporters that are essential for productive N2-fixation in peas (Hosie et al., 2001; Hosie et al., 2002; Lodwig et al., 2003; Prell & Poole, 2006; White et al., 2007) and in recent ground-breaking work we have concluded they secrete alanine as part of a GABA-alanine-cycle operating in nodules. The oxo-acid generated by this cycle is succinate semialdehyde, which our calculations reveal has one of the lowest redox potentials of any compound in intermediary metabolism. Its midpoint potential is less than that of ferredoxin and is therefore ideally suited for the generation of reductant for nitrogenase. Furthermore, the GABA-alanine cycle bypasses a large part of the TCA-cycle, suggesting that central metabolism may be completely changed in legume bacteroids; explaining why many TCA-cycle mutants are able to fix nitrogen in planta. It is crucial to determine whether the GABA-alanine- and TCA-cycles function alone or in combination to drive N2-fixation in Rhizobium-legume symbioses to underpin the global nitrogen cycle. We therefore propose to determine how GABA is metabolised to balance bacteroid metabolism and fuel N2-fixation, and how this is integrated with the TCA-cycle. The very low midpoint potential of succinate semialdehyde is an extraordinarily powerful reason for bacteroid metabolism to use semialdehyde oxidation and may be important in generating a global redox switch for N2-fixation in legume nodules. Thus, the GABA-alanine-cycle may explain both how carbon and nitrogen are exchanged between the plant and bacterial symbionts, as well as provide a powerful chemical rationale for how reduction of N2 is achieved.
Organisations
- John Innes Centre (Lead Research Organisation)
- University of Wisconsin-Madison (Collaboration)
- James Hutton Institute (Collaboration)
- ABERYSTWYTH UNIVERSITY (Collaboration)
- University of Calcutta (Collaboration)
- Montana State University (Collaboration)
- IMPERIAL COLLEGE LONDON (Collaboration)
- Massachusetts Institute of Technology (Collaboration)
- University of Hyderabad (Collaboration)
- Indian Council of Agricultural Research (Collaboration)
- John Innes Centre (Collaboration)
- Plant Biology Division (Collaboration)
- THE ENERGY AND RESOURCES INSTITUTE (Collaboration)
- Oxford India Centre for Sustainable Development (OICSD) (Collaboration)
- Maharaja Sayajirao University of Baroda (Collaboration)
People |
ORCID iD |
Philip Poole (Principal Investigator) |
Publications
Terpolilli JJ
(2012)
What determines the efficiency of N(2)-fixing Rhizobium-legume symbioses?
in Advances in microbial physiology
Oldroyd GE
(2011)
The rules of engagement in the legume-rhizobial symbiosis.
in Annual review of genetics
Udvardi M
(2013)
Transport and metabolism in legume-rhizobia symbioses.
in Annual review of plant biology
Karunakaran R
(2013)
Malonate catabolism does not drive N2 fixation in legume nodules.
in Applied and environmental microbiology
Zhang Y
(2012)
NAD(P)+-malic enzyme mutants of Sinorhizobium sp. strain NGR234, but not Azorhizobium caulinodans ORS571, maintain symbiotic N2 fixation capabilities.
in Applied and environmental microbiology
Hood G
(2017)
Manganese transport is essential for N2 -fixation by Rhizobium leguminosarum in bacteroids from galegoid but not phaseoloid nodules.
in Environmental microbiology
Terpolilli JJ
(2016)
Lipogenesis and Redox Balance in Nitrogen-Fixing Pea Bacteroids.
in Journal of bacteriology
Prell J
(2009)
Pathway of gamma-aminobutyrate metabolism in Rhizobium leguminosarum 3841 and its role in symbiosis.
in Journal of bacteriology
Mulley G
(2010)
Pyruvate is synthesized by two pathways in pea bacteroids with different efficiencies for nitrogen fixation.
in Journal of bacteriology
White JP
(2009)
Characterization of a {gamma}-aminobutyric acid transport system of Rhizobium leguminosarum bv. viciae 3841.
in Journal of bacteriology
Mulley G
(2011)
Mutation of GOGAT prevents pea bacteroid formation and N2 fixation by globally downregulating transport of organic nitrogen sources.
in Molecular microbiology
Schulte CCM
(2022)
Genome-Scale Metabolic Modelling of Lifestyle Changes in Rhizobium leguminosarum.
in mSystems
Poole P
(2018)
Rhizobia: from saprophytes to endosymbionts.
in Nature reviews. Microbiology
Prell J
(2010)
Role of symbiotic auxotrophy in the Rhizobium-legume symbioses.
in PloS one
Poole P
(2017)
Shining a light on the dark world of plant root-microbe interactions.
in Proceedings of the National Academy of Sciences of the United States of America
Prell J
(2009)
Legumes regulate Rhizobium bacteroid development and persistence by the supply of branched-chain amino acids.
in Proceedings of the National Academy of Sciences of the United States of America
Schulte C
(2021)
Metabolic control of nitrogen fixation in rhizobium-legume symbioses
in Science Advances
Terpolilli J
(2014)
Genome sequence of Rhizobium leguminosarum bv trifolii strain WSM1689, the microsymbiont of the one flowered clover Trifolium uniflorum.
in Standards in genomic sciences
Description | Bacteria are simple single celled organisms that lack the membrane-bound structures found in higher cells of plants and animals. However, while bacteria may have a less complex cellular organisation they carry out a huge range of chemical reactions not found in plants and animals. Bacteria are responsible for the cycling of many nutrients such as nitrogen, which is a very inert atmospheric gas. Although nitrogen makes up 78% of the atmosphere it cannot be used directly as a source of nitrogen, which is needed for amino acid, protein and DNA synthesis. However, a small number of bacteria can reduce (add hydrogen) to nitrogen and convert it into ammonia (NH3), which is readily incorporated into amino acids and then all the other building blocks of life, by a wide range of organisms including animals and plants. In many parts of the world the limitation to growth of plants, which in turn support animal life, is the supply of nitrogen as ammonia or related compounds. Since up to 65% of available nitrogen (e.g. ammonia) comes from bacteria this makes them essential for life on earth. Within the bacteria, most of the nitrogen is actually produced by one family known as the Rhizobiacea. This remarkable group of bacteria form a symbiotic association (both partners benefit) with plants of the legume family, that results in the formation of root nodules (on pea plants these are 2-3 mm 'bulbs' that can easily be seen by pulling up a plant and inspecting its roots). The rhizobia are held inside the nodules where the plant provides them with an ideal environment in which they can produce ammonia. The ammonia is supplied to the plant as its nitrogen source so this is why this is known as a symbiotic interaction. It means that the plant does need any nitrogen in the soil and enables rapid growth. The purpose of this research is to understand the type of fuel provided by the plant to power the fixation of nitrogen to ammonia by the bacteria. We have performed experiments to map bacterial metabolism leading to a greater understanding of the symbiotic process. |
Exploitation Route | This work forms a basis for extending nitrogen-fixation to non-legume (crop) plants. |
Sectors | Agriculture, Food and Drink |
Description | BioSciences DTP, University of Oxford |
Amount | £80,000 (GBP) |
Funding ID | 1211269 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 09/2018 |
End | 09/2022 |
Description | Continuation of ENSA (RL) |
Amount | $226,932 (USD) |
Organisation | Foreign Commonwealth and Development Office (FCDO) |
Sector | Public |
Country | United Kingdom |
Start | 01/2021 |
End | 03/2024 |
Description | ENGINEERING SYNTHETIC SYMBIOSES BETWEEN PLANTS AND BACTERIA TO DELIVER NITROGEN TO CROPS |
Amount | £1,146,829 (GBP) |
Funding ID | BB/L011484/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 08/2013 |
End | 08/2018 |
Description | Factors controlling N2-fixing ability and competitiveness of rhizobia to nodulate legumes |
Amount | £784,606 (GBP) |
Funding ID | BB/W006219/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 02/2022 |
End | 01/2025 |
Description | India-UK Nitrogen Fixation Centre (IUNFC) |
Amount | £1,680,948 (GBP) |
Funding ID | BB/N013387/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 01/2016 |
End | 12/2018 |
Description | Managing the Nitrogen economy of bacteria |
Amount | £4,496,752 (GBP) |
Funding ID | BB/N003608/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 01/2016 |
End | 01/2020 |
Description | Managing the Nitrogen economy of bacteria (Co-I with Prof. Martin Buck as Lead PI) |
Amount | £4,496,752 (GBP) |
Funding ID | BB/N003608/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 01/2016 |
End | 01/2020 |
Description | Marie Curie (IA) |
Amount | € 224,933 (EUR) |
Organisation | Marie Curie |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 05/2021 |
End | 06/2023 |
Description | Mechanism of global regulation of ATP dependent transporters by PTS-NTR |
Amount | £520,908 (GBP) |
Funding ID | BB/K006134/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 03/2013 |
End | 02/2016 |
Description | Metabolic bottlenecks in rhizobia affecting competitive nodulation during infection stages |
Amount | SFr. 103,400 (CHF) |
Funding ID | P400PB_183901 / 1 |
Organisation | Swiss National Science Foundation |
Sector | Public |
Country | Switzerland |
Start | 03/2019 |
End | 02/2021 |
Description | Rhizobium bacteroid development |
Amount | £556,695 (GBP) |
Funding ID | BB/J007749/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 04/2012 |
End | 04/2015 |
Description | Understanding and Exploiting Biological Nitrogen Fixation for Improvement of Brazilian Agriculture (Co-I with Prof. Ray Dixon as Lead PI) |
Amount | £1,624,659 (GBP) |
Funding ID | BB/N013476/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 02/2016 |
End | 01/2019 |
Description | Brazil and nitrogen fixation |
Organisation | Aberystwyth University |
Department | Institute of Biological, Environmental and Rural Sciences (IBERS) |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Our emphasis is to study the physiology of bacterial growth and survival in the rhizosphere and how they establish symbiotic interactions with plants. A further focus of our work is the physiology and biochemistry of nitrogen fixation in legume nodules. Most recently we have been studying how bacteria attach to and colonise roots and have developed methods to open up the whole area of how plants control the microbial root microbiome. |
Collaborator Contribution | Our major emphasis is on signal transduction cascades that regulate nitrogen fixation genes in response to oxygen, carbon and fixed nitrogen status. A long-term goal of this research is to exploit and engineer nitrogen fixation genes for agricultural benefit. (JIC) Research in the Farrar lab focuses on increasing Miscanthus yields in order to replace fossil fuel usage, sequester atmospheric carbon, and ultimately contribute to climate change mitigation. In order to achieve this, there are two main research areas: Plant developmental biology & genetics and Plant-soil-microbe interactions. (IBERS) Plant electron microscopy: the ultrastructure of beneficial plant-bacterial interactions. I also specialise in immunolocalisation of both conventionally (chemically)- and cryo-fixed plant material. Expertise in nitrogen fixation by legumes and non-legumes (JHI) |
Impact | Grant awarded from BBSRC Newton Fund. Grant Ref: BB/N013476/1 Understanding and Exploiting Biological Nitrogen Fixation for Improvement of Brazilian Agriculture. PI: Prof. Ray Dixon |
Start Year | 2014 |
Description | Brazil and nitrogen fixation |
Organisation | James Hutton Institute |
Country | United Kingdom |
Sector | Charity/Non Profit |
PI Contribution | Our emphasis is to study the physiology of bacterial growth and survival in the rhizosphere and how they establish symbiotic interactions with plants. A further focus of our work is the physiology and biochemistry of nitrogen fixation in legume nodules. Most recently we have been studying how bacteria attach to and colonise roots and have developed methods to open up the whole area of how plants control the microbial root microbiome. |
Collaborator Contribution | Our major emphasis is on signal transduction cascades that regulate nitrogen fixation genes in response to oxygen, carbon and fixed nitrogen status. A long-term goal of this research is to exploit and engineer nitrogen fixation genes for agricultural benefit. (JIC) Research in the Farrar lab focuses on increasing Miscanthus yields in order to replace fossil fuel usage, sequester atmospheric carbon, and ultimately contribute to climate change mitigation. In order to achieve this, there are two main research areas: Plant developmental biology & genetics and Plant-soil-microbe interactions. (IBERS) Plant electron microscopy: the ultrastructure of beneficial plant-bacterial interactions. I also specialise in immunolocalisation of both conventionally (chemically)- and cryo-fixed plant material. Expertise in nitrogen fixation by legumes and non-legumes (JHI) |
Impact | Grant awarded from BBSRC Newton Fund. Grant Ref: BB/N013476/1 Understanding and Exploiting Biological Nitrogen Fixation for Improvement of Brazilian Agriculture. PI: Prof. Ray Dixon |
Start Year | 2014 |
Description | Brazil and nitrogen fixation |
Organisation | John Innes Centre |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Our emphasis is to study the physiology of bacterial growth and survival in the rhizosphere and how they establish symbiotic interactions with plants. A further focus of our work is the physiology and biochemistry of nitrogen fixation in legume nodules. Most recently we have been studying how bacteria attach to and colonise roots and have developed methods to open up the whole area of how plants control the microbial root microbiome. |
Collaborator Contribution | Our major emphasis is on signal transduction cascades that regulate nitrogen fixation genes in response to oxygen, carbon and fixed nitrogen status. A long-term goal of this research is to exploit and engineer nitrogen fixation genes for agricultural benefit. (JIC) Research in the Farrar lab focuses on increasing Miscanthus yields in order to replace fossil fuel usage, sequester atmospheric carbon, and ultimately contribute to climate change mitigation. In order to achieve this, there are two main research areas: Plant developmental biology & genetics and Plant-soil-microbe interactions. (IBERS) Plant electron microscopy: the ultrastructure of beneficial plant-bacterial interactions. I also specialise in immunolocalisation of both conventionally (chemically)- and cryo-fixed plant material. Expertise in nitrogen fixation by legumes and non-legumes (JHI) |
Impact | Grant awarded from BBSRC Newton Fund. Grant Ref: BB/N013476/1 Understanding and Exploiting Biological Nitrogen Fixation for Improvement of Brazilian Agriculture. PI: Prof. Ray Dixon |
Start Year | 2014 |
Description | India and nitrogen fixation |
Organisation | Indian Council of Agricultural Research |
Department | ICAR - Indian Agricultural Research Institute, New Delhi |
Country | India |
Sector | Private |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | Indian Council of Agricultural Research |
Department | ICAR-Indian Institute of Soil Science |
Country | India |
Sector | Learned Society |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | James Hutton Institute |
Country | United Kingdom |
Sector | Charity/Non Profit |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | John Innes Centre |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | Maharaja Sayajirao University of Baroda |
Department | Department of Bio-Chemistry |
Country | India |
Sector | Academic/University |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | Oxford India Centre for Sustainable Development (OICSD) |
Country | United Kingdom |
Sector | Public |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | The Energy and Resources Institute |
Country | India |
Sector | Charity/Non Profit |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | University of Calcutta |
Department | Department of BioChemistry |
Country | India |
Sector | Academic/University |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | India and nitrogen fixation |
Organisation | University of Hyderabad |
Department | Department of Plant Sciences |
Country | India |
Sector | Academic/University |
PI Contribution | I have developed high-throughput community analysis to characterise the microbiome of the rhizosphere and endosphere of plants. Using the latest techniques in synthetic biology I have developed high-throughput screening techniques to assess rhizobial competitiveness and ability to fix nitrogen. We have developed biosensor reporter technology linked to Lux imaging of whole roots to assess root secretion and its impact on microbial colonization and nitrogen fixation. |
Collaborator Contribution | University of Hyderabad- experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Institute of Soil Science, Bhopal, Madhya Pradesh- experience with different Indian soils and agricultural expertise of growth of pigeon pea. Also experience in metagenomic diversity of rhizospheric microbes of major crops. M.S.University of Baroda,Vadodara, Gujarat - experience with different Indian soils and agricultural expertise of growth of pigeon pea ICAR-Indian Agricultural Research Institute, New Delhi - interaction with India subsistence farmers and legume-Rhizobium symbiosis under salt and nitrate stress. Also expertise on coordinating microbiological trials in different agroclimatic zones in India. University of Calcutta - expertise in nitrogen-fixing root nodule symbiosis in Arachis hypogaea. The Energy and Resources Institute, New Delhi - bioengineering of rice for symbiotic nitrogen fixation. John Innes Centre - world-leading expertise in the molecular biology of nitrogen fixation and biochemical characterisation of its transcriptional control. James Hutton Institute - specialised in nitrogen fixation by legumes and non-legumes, particularly those from the tropics. Techniques include high-resolution microscopy (light and EM) and 15N-based methods, which in combination have been used to confirm the effectiveness of many novel nitrogen-fixing plant-bacterial interactions Oxford India Centre for Sustainable Development (OICSD)-(inter-disciplinary) expertise on Indian life, cultural and practices from the aspect of sustainable development. Examining the interactions and trade-offs between different players in socio-ecological systems, especially in the Indian food system. |
Impact | A successful application was made to BBSRC-DBT as part of the Newton initiative by this group, led by Prof. Philip Poole. We have set up a VJC - the India-UK Nitrogen Fixation Centre (IUNFC) which was funded from Jan 1st 2016 in UK. This funding is likely to lead to many outputs, and as this collaboration is multi-disciplinary, in many areas, however as the project has only just begun, these are yet to be realised. |
Start Year | 2016 |
Description | Nitrogen economy of bacteria |
Organisation | Imperial College London |
Department | Imperial College Trust |
Country | United Kingdom |
Sector | Charity/Non Profit |
PI Contribution | Emphasis is to study the physiology of bacterial growth and survival in the rhizosphere and how they establish symbiotic interactions with plants. A further focus of our work is the physiology and biochemistry of nitrogen fixation in legume nodules. |
Collaborator Contribution | Focus on establishing the mechanistic basis of signal perception and propagation that operates to allow the appropriate adaptive patterns of gene expression to be mounted. Study of bacterial systems, of agronomic, medical and industrial relevance. Computational systems biology, drawing together information from macromolecular sequences, protein structures, biological networks and other experimental data to study the evolution and function of biological systems. Emphasis on signal transduction cascades that regulate nitrogen fixation genes in response to oxygen, carbon and fixed nitrogen status. A long-term goal of this research is to exploit and engineer nitrogen fixation genes for agricultural benefit. |
Impact | Funding obtained from BBSRC for sLoLa 'Managing the Nitrogen economy of bacteria' Grant Ref: BB/N003608/1. 2nd Jan 2016-1st Jan 2020 |
Start Year | 2014 |
Description | Nitrogen economy of bacteria |
Organisation | Imperial College London |
Department | Imperial College Trust |
Country | United Kingdom |
Sector | Charity/Non Profit |
PI Contribution | Emphasis is to study the physiology of bacterial growth and survival in the rhizosphere and how they establish symbiotic interactions with plants. A further focus of our work is the physiology and biochemistry of nitrogen fixation in legume nodules. |
Collaborator Contribution | Focus on establishing the mechanistic basis of signal perception and propagation that operates to allow the appropriate adaptive patterns of gene expression to be mounted. Study of bacterial systems, of agronomic, medical and industrial relevance. Computational systems biology, drawing together information from macromolecular sequences, protein structures, biological networks and other experimental data to study the evolution and function of biological systems. Emphasis on signal transduction cascades that regulate nitrogen fixation genes in response to oxygen, carbon and fixed nitrogen status. A long-term goal of this research is to exploit and engineer nitrogen fixation genes for agricultural benefit. |
Impact | Funding obtained from BBSRC for sLoLa 'Managing the Nitrogen economy of bacteria' Grant Ref: BB/N003608/1. 2nd Jan 2016-1st Jan 2020 |
Start Year | 2014 |
Description | Nitrogen economy of bacteria |
Organisation | John Innes Centre |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Emphasis is to study the physiology of bacterial growth and survival in the rhizosphere and how they establish symbiotic interactions with plants. A further focus of our work is the physiology and biochemistry of nitrogen fixation in legume nodules. |
Collaborator Contribution | Focus on establishing the mechanistic basis of signal perception and propagation that operates to allow the appropriate adaptive patterns of gene expression to be mounted. Study of bacterial systems, of agronomic, medical and industrial relevance. Computational systems biology, drawing together information from macromolecular sequences, protein structures, biological networks and other experimental data to study the evolution and function of biological systems. Emphasis on signal transduction cascades that regulate nitrogen fixation genes in response to oxygen, carbon and fixed nitrogen status. A long-term goal of this research is to exploit and engineer nitrogen fixation genes for agricultural benefit. |
Impact | Funding obtained from BBSRC for sLoLa 'Managing the Nitrogen economy of bacteria' Grant Ref: BB/N003608/1. 2nd Jan 2016-1st Jan 2020 |
Start Year | 2014 |
Description | SynSym Project |
Organisation | John Innes Centre |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Bacterial physiology and genetics of nitrogen-fixing symbiotic microorganisms. |
Collaborator Contribution | Expertise in genetics of legumes (JIC and University of Wisconsin), the enzyme nitrogenase, responsible for fixing nitrogen in both aerobic and anaerobic bacteria (Montana State university), plant physiology (Noble Foundation) and synthetic biology (MIT). We are working together on a project - Synthetic Symbioses - which aims to use synthetic biology to enable bacteria associating with plant roots to fix nitrogen. This symbiotic association, where the bacteria fixes nitrogen and benefits the plant, occurs naturally between legumes (plants) and rhizobia (bacteria). |
Impact | A research grant from BBSRC/NSF was obtained to study Synthetic Symbioses. GRANT TITLE: ENGINEERING SYNTHETIC SYMBIOSES BETWEEN PLANTS AND BACTERIA TO DELIVER NITROGEN TO CROPS Grant Ref: BB/L011484/1 |
Start Year | 2013 |
Description | SynSym Project |
Organisation | Massachusetts Institute of Technology |
Country | United States |
Sector | Academic/University |
PI Contribution | Bacterial physiology and genetics of nitrogen-fixing symbiotic microorganisms. |
Collaborator Contribution | Expertise in genetics of legumes (JIC and University of Wisconsin), the enzyme nitrogenase, responsible for fixing nitrogen in both aerobic and anaerobic bacteria (Montana State university), plant physiology (Noble Foundation) and synthetic biology (MIT). We are working together on a project - Synthetic Symbioses - which aims to use synthetic biology to enable bacteria associating with plant roots to fix nitrogen. This symbiotic association, where the bacteria fixes nitrogen and benefits the plant, occurs naturally between legumes (plants) and rhizobia (bacteria). |
Impact | A research grant from BBSRC/NSF was obtained to study Synthetic Symbioses. GRANT TITLE: ENGINEERING SYNTHETIC SYMBIOSES BETWEEN PLANTS AND BACTERIA TO DELIVER NITROGEN TO CROPS Grant Ref: BB/L011484/1 |
Start Year | 2013 |
Description | SynSym Project |
Organisation | Montana State University |
Country | United States |
Sector | Academic/University |
PI Contribution | Bacterial physiology and genetics of nitrogen-fixing symbiotic microorganisms. |
Collaborator Contribution | Expertise in genetics of legumes (JIC and University of Wisconsin), the enzyme nitrogenase, responsible for fixing nitrogen in both aerobic and anaerobic bacteria (Montana State university), plant physiology (Noble Foundation) and synthetic biology (MIT). We are working together on a project - Synthetic Symbioses - which aims to use synthetic biology to enable bacteria associating with plant roots to fix nitrogen. This symbiotic association, where the bacteria fixes nitrogen and benefits the plant, occurs naturally between legumes (plants) and rhizobia (bacteria). |
Impact | A research grant from BBSRC/NSF was obtained to study Synthetic Symbioses. GRANT TITLE: ENGINEERING SYNTHETIC SYMBIOSES BETWEEN PLANTS AND BACTERIA TO DELIVER NITROGEN TO CROPS Grant Ref: BB/L011484/1 |
Start Year | 2013 |
Description | SynSym Project |
Organisation | Plant Biology Division |
Country | United States |
Sector | Private |
PI Contribution | Bacterial physiology and genetics of nitrogen-fixing symbiotic microorganisms. |
Collaborator Contribution | Expertise in genetics of legumes (JIC and University of Wisconsin), the enzyme nitrogenase, responsible for fixing nitrogen in both aerobic and anaerobic bacteria (Montana State university), plant physiology (Noble Foundation) and synthetic biology (MIT). We are working together on a project - Synthetic Symbioses - which aims to use synthetic biology to enable bacteria associating with plant roots to fix nitrogen. This symbiotic association, where the bacteria fixes nitrogen and benefits the plant, occurs naturally between legumes (plants) and rhizobia (bacteria). |
Impact | A research grant from BBSRC/NSF was obtained to study Synthetic Symbioses. GRANT TITLE: ENGINEERING SYNTHETIC SYMBIOSES BETWEEN PLANTS AND BACTERIA TO DELIVER NITROGEN TO CROPS Grant Ref: BB/L011484/1 |
Start Year | 2013 |
Description | SynSym Project |
Organisation | University of Wisconsin-Madison |
Country | United States |
Sector | Academic/University |
PI Contribution | Bacterial physiology and genetics of nitrogen-fixing symbiotic microorganisms. |
Collaborator Contribution | Expertise in genetics of legumes (JIC and University of Wisconsin), the enzyme nitrogenase, responsible for fixing nitrogen in both aerobic and anaerobic bacteria (Montana State university), plant physiology (Noble Foundation) and synthetic biology (MIT). We are working together on a project - Synthetic Symbioses - which aims to use synthetic biology to enable bacteria associating with plant roots to fix nitrogen. This symbiotic association, where the bacteria fixes nitrogen and benefits the plant, occurs naturally between legumes (plants) and rhizobia (bacteria). |
Impact | A research grant from BBSRC/NSF was obtained to study Synthetic Symbioses. GRANT TITLE: ENGINEERING SYNTHETIC SYMBIOSES BETWEEN PLANTS AND BACTERIA TO DELIVER NITROGEN TO CROPS Grant Ref: BB/L011484/1 |
Start Year | 2013 |
Description | 21st Congress on Nitrogen Fixation - 10th-15th Oct 2019, Wuhan, China - Philip Poole |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Phil gave a talk at this international conference. He had many questions on his work and spent time exchanging ideas with colleagues in this research area. |
Year(s) Of Engagement Activity | 2019 |
URL | http://2019icnf.csp.escience.cn/dct/page/65580 |
Description | 5th Annual Missouri University Plant Research Symposium |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Phil gave a talk entitled 'Improving legume symbiosis or engineering cereals' in this virtual symposium: The Next Green Revolution: Challenges and Strategies towards a Sustainable Agriculture. |
Year(s) Of Engagement Activity | 2021 |
URL | https://www.corteva.com/our-impact/innovation/symposiaseries.html |
Description | BASF Symposium 'Unlocking Yield Potential in Soil', Germany, October 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | Yes |
Type Of Presentation | keynote/invited speaker |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Talk provided plenty of questions from audience. Increase interest in my work. |
Year(s) Of Engagement Activity | 2014 |
URL | http://rhizosphere.org/lab-news/ |
Description | Back2Roots Advisor, annual project meeting. Jan 2017 |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | International advisor to the Back2Roots research programme in the Netherlands |
Year(s) Of Engagement Activity | 2016 |
Description | Bacterial World, Natural History Museum, Oxford, Dec 2018 (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Schools |
Results and Impact | Phil gave a talk to school children on an open day at the museum as part of the Bacterial World exhibition. |
Year(s) Of Engagement Activity | 2018 |
URL | http://www.oum.ox.ac.uk/bacterialworld/ |
Description | Bayer Crop Science 16th-17th Feb 2016 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Bayer Crop Science 16th-17th Feb 2016. A group of academics advised industry |
Year(s) Of Engagement Activity | 2016 |
Description | Blog post for Microbiology Society (CS) |
Form Of Engagement Activity | A magazine, newsletter or online publication |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Carolin wrote an article entitled Fixing the Problem of Nitrogen Limitation in Agriculture for the Microbiology Society 75th Anniversary publication on a Sustainable Future. |
Year(s) Of Engagement Activity | 2020 |
URL | https://microbiologysociety.org/our-work/75th-anniversary-a-sustainable-future/soil-health/soil-heal... |
Description | Botanic Gardens seminar |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | I talked to the Botanic Gardens about nitrogen and humanity Lots of questions and feedback. This is widely advertised to the public |
Year(s) Of Engagement Activity | 2014 |
Description | Bring and share pulse lunch |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Postgraduate students |
Results and Impact | Bring and share pulse lunch to celebrate world pulse day 18th Jan 2017 |
Year(s) Of Engagement Activity | 2017 |
URL | https://rhizosphere.org/lab-news/ |
Description | Centre of Excellence for Plant and Microbial Science (CEPAMS), Shanghai, China - Philip Poole (Oct 2019) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | Phil visited Dr Jeremy Murray at CEPAMS and gave a talk to scientists and students. |
Year(s) Of Engagement Activity | 2019 |
URL | https://rhizosphere.org/lab-news/ |
Description | Development of Poole Lab website (Rhizosphere.org) |
Form Of Engagement Activity | Engagement focused website, blog or social media channel |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | The Rhizosphere website is used to describe work performed, profiles lab members, lists outreach activities and celebrates achievements. Also includes a list of Lab publications. |
Year(s) Of Engagement Activity | 2014,2015,2016,2017,2018,2019,2020 |
URL | https://rhizosphere.org |
Description | ENFC, Budapest posters and talks |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | An international conference. Group members presented posters and gave talks to approx. 500 scientists |
Year(s) Of Engagement Activity | 2016 |
URL | https://rhizosphere.org/lab-news/ |
Description | European Nitrogen Fixation Conference (ENFC), Spain, Sept 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | Yes |
Type Of Presentation | keynote/invited speaker |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Talk led to plenty of discussion. The six posters from the group which were also presented were well attended and provoked interest. Improved collaboration with international colleagues. |
Year(s) Of Engagement Activity | 2014 |
URL | http://rhizosphere.org/lab-news/ |
Description | Facebook LIVE video by Oxford Sparks |
Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | Oxford Sparks FacebookLIVE podcast on nitrogen and the importance of nitrogen-fixing legumes |
Year(s) Of Engagement Activity | 2016 |
URL | https://www.facebook.com/OxSparks/videos/1210777428999882/ |
Description | FacebookLIVE - the future of food |
Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Schools |
Results and Impact | Facebook LIVE podcast in association with Oxford Sparks (latest 6.6k views) |
Year(s) Of Engagement Activity | 2017 |
URL | https://www.facebook.com/OxSparks/videos/1210777428999882/ |
Description | Gatsby Plant Science Summer School, Hawkshill, July 2018 (Phil Poole, Vinoy Ramachandran) |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Undergraduate students |
Results and Impact | Summer school for undergraduates interested in Plant Sciences. Phil gave a talk. Vinoy attended as a post-doc mentor to the undergraduates. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Give peas a chance |
Form Of Engagement Activity | A magazine, newsletter or online publication |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Schools |
Results and Impact | We made a film with Oxford sparks on nitrogen fixation Lots os hits on the web site |
Year(s) Of Engagement Activity | 2014 |
Description | Graduate School Speaker, Lunteren, The Netherlands, April 2017 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Graduate School Speaker, Lunteren, The Netherlands, April 2017 |
Year(s) Of Engagement Activity | 2017 |
Description | International Conference on Nitrogen Fixation (ICNF) Asilomar, USA, Oct 2015 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | Yes |
Type Of Presentation | keynote/invited speaker |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Talk led to discussions. Poster sparked interest amongst audience. Interest and approval of work from respected colleagues. Discussion of new ideas in the field. |
Year(s) Of Engagement Activity | 2015 |
URL | http://rhizosphere.org/lab-news/ |
Description | KEYS conference, Amsterdam, June 2018 (Phil Poole) |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Industry/Business |
Results and Impact | Gave a talk to an industry-sponsored conference. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Lecture at Cirencester Science and Technology Society, Royal Agricultural University, 9th May 2018, (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | Lecture given to general public interested in science. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Max Planck Institute for Plant Breeding Research, Cologne, Germany Dec 2018 (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Study participants or study members |
Results and Impact | Seminar and discussion with colleagues at Max Planck Institue. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Meeting minds Oxford Alumni |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | I gave a talk to the Oxford biological sciences Alumni Enthusiastic response from the audience |
Year(s) Of Engagement Activity | 2014 |
Description | Michigan State University, student workshop |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | Student workshop at an American University where I was an international expert. |
Year(s) Of Engagement Activity | 2016 |
Description | Mini-symposium Chemical Signalling in the Rhizosphere (Cumbria, Sept. 2019)- Phil Poole |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | A group of international scientists focussed on a very defined problem in a retreat-like setting. |
Year(s) Of Engagement Activity | 2019 |
URL | https://rhizosphere.org/lab-news/ |
Description | NIAB-East Malling Research seminar series, Jan 2019 (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Postgraduate students |
Results and Impact | Lunch time seminar at Research Institute. Spent much of the day in discussion with colleagues there. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Organised OxBacNet meeting - Feb 2018 (Alison East) |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Professional Practitioners |
Results and Impact | University of Oxford, Bacteriology Symposium and Network meeting. Held three times a year. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/oxbacnet/ |
Description | Oxford Botanic Gardens Seminar Series, Nov 2018 (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | Evening seminar to interested members of general public. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Plant Genomes, Systems Biology and Engineering, Cold Spring Harbor, 1st-3rd Dec 2021 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | On-line conference. Plant Genomes, Systems Biology and Engineering. |
Year(s) Of Engagement Activity | 2021 |
URL | https://meetings.cshl.edu/meetings.aspx?meet=PLANTS&year=21 |
Description | Plant-Microbe Interactions Symposium, Society for Applied Microbiology, Oct 2020 (PSP) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Phil gave a talk entitled 'Rhizobium from rhizosphere to root nodule' at this online conference. It reached a wide audience who asked a series of questions after the talk. |
Year(s) Of Engagement Activity | 2020 |
URL | https://sfam.org.uk/career/ems-event-calendar/plant-microbe-interactions-series-part-1.html |
Description | Reatch Nano-talks, Sustainability and Microbes, April 2020 (CS) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Carolin gave a presentation which was shared online. It can still be viewed in the archive |
Year(s) Of Engagement Activity | 2020 |
URL | http://reatch.ch/events/april-nanotalks-sustainability-microbes |
Description | Research Seminar, Lisbon, March 2017 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Research Seminar, Lisbon, March 2017 at II Semana Bioengenharia, Instuto Superior Tecnico, Lisbon, Portugal |
Year(s) Of Engagement Activity | 2017 |
URL | https://rhizosphere.org/lab-news/ |
Description | Rothamsted Research invited seminar - Phil Poole |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | Phil gave a lunch-time research seminar. |
Year(s) Of Engagement Activity | 2019 |
URL | https://rhizosphere.org/lab-news/ |
Description | School of Life Sciences, University of Warwick, UK. Seminar, 29th April 2016 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Postgraduate students |
Results and Impact | School of Life Sciences, University of Warwick, UK. Seminar, 29th April 2016, addressed audience made up of academic colleagues and post-grad students |
Year(s) Of Engagement Activity | 2016 |
Description | Schools one week Intensive course |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Schools |
Results and Impact | Intensive one week course for A level students in practical plant and microbial science Students all keen to apply for science based university courses |
Year(s) Of Engagement Activity | 2014 |
Description | Seminar, University of Southampton, Feb 2017 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Postgraduate students |
Results and Impact | Seminar, Crop Systems Engineering Seminar Series, University of Southampton, Feb 2017 |
Year(s) Of Engagement Activity | 2017 |
Description | Society for General Microbiology 'Emerging Challenges and Opportunities in Soil Microbiology', Loughborough, Sept. 2014 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | Yes |
Type Of Presentation | paper presentation |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Talk led to discussions Audience showed interest and discussions were had with colleagues. |
Year(s) Of Engagement Activity | 2014 |
URL | http://rhizosphere.org/lab-news/ |
Description | Symbiosis Day at Museum (April 2019) - Carolin Schulte and Brandon Ford |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | Brandon and Carolin had a display (including 3D-printed bacteroids!) to engage with the public on the subject of nitrogen fixation and symbiosis. |
Year(s) Of Engagement Activity | 2019 |
URL | https://rhizosphere.org/lab-news/ |
Description | Talk at Curtin University, Western Australia - Phil Poole (Nov 2019) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | Phil gave a seminar on his research to an audience of scientists. |
Year(s) Of Engagement Activity | 2019 |
URL | https://rhizosphere.org/lab-news/ |
Description | Talk at OxBacNet Oct 2021 (RL) |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Postgraduate students |
Results and Impact | Presentation to OxBacNet members |
Year(s) Of Engagement Activity | 2021 |
Description | Talk to Zoological Society of London, London Zoo,13th March 2018 (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | Seminar to an audience interested in science, but not specialists in the field. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Thatcher Development Programme's Foundation Day Panel Discussion, 'Climate Change: Approaches and Innovations for the Future' - Feb 208 (Phil Poole) |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Undergraduate students |
Results and Impact | Broad-ranging panel discussion with students from different disciplines held at Somerville College, Oxford. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | The Microbiome: human medicine and agriculture in a bacterial world, Royal Society, Oct 2018 (Phil Poole) |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | Royal Society organised two days of discussion to formulate policy. |
Year(s) Of Engagement Activity | 2018 |
Description | The Queen's College, seminar series (Carmen Sanchez-Canizares) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Other audiences |
Results and Impact | The Queen's College, seminar series (Carmen Sanchez-Canizares) Title: Life Underground: The basis and future of agriculture |
Year(s) Of Engagement Activity | 2017 |
URL | https://rhizosphere.org/lab-news/ |
Description | Twitter account Rhizosphere @PooleLabOxford |
Form Of Engagement Activity | Engagement focused website, blog or social media channel |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | Twitter account to publicise the work of the Poole Lab |
Year(s) Of Engagement Activity | 2017,2018,2019,2020 |
URL | https://twitter.com |
Description | University of York, Dept. of Biology invited seminar- Feb 2018 (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Postgraduate students |
Results and Impact | Seminar and active discussion with members of Dept. of Biology |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | VI Annual Meeting of the Plant Genetic and Biotechnology Network, Graduate School, Italy, June 2017 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | VI Annual Meeting of the Plant Genetic and Biotechnology Network, Graduate School, Italy, June 2017 |
Year(s) Of Engagement Activity | 2017 |
URL | https://rhizosphere.org/lab-news/ |
Description | Visit to Centre of Rhizobium Studies, Murdoch University, Perth - Philip Poole (Nov 2019) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Postgraduate students |
Results and Impact | Phil visited CRS and gave a seminar. Also during his visit he was able to interact with post-graduate students. |
Year(s) Of Engagement Activity | 2019 |
URL | https://rhizosphere.org/lab-news/ |
Description | Visit to Murdoch University, WA |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Industry/Business |
Results and Impact | Spent 1 month in Australia as Sir Walter Murdoch Adjunct Professor with Australian colleagues. Liaising, gave a seminar and got involved with their research activities. |
Year(s) Of Engagement Activity | 2016 |
Description | Visit to Murdoch University, Western Australia as Sir Walter Murdoch Visiting Fellow, Dec 2014 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Postgraduate students |
Results and Impact | Visit to another laboratory for several weeks. Included giving two seminars and extended discussions with colleagues in the field. Strong ties formed and fostered with the research groups in universities in Western Australia. |
Year(s) Of Engagement Activity | 2014 |
URL | http://rhizosphere.org/lab-news/ |
Description | Winchester College, Nov 2018 (Phil Poole) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Schools |
Results and Impact | Phil gave a talk to the students of Winchester College. |
Year(s) Of Engagement Activity | 2018 |
URL | https://rhizosphere.org/lab-news/ |
Description | Wow! How? Family Science Fair, March 2013, 2014, 2015 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | Yes |
Geographic Reach | Local |
Primary Audience | Schools |
Results and Impact | Demonstrations stimulated discussion about simple science with children aged 5-10yrs and their parents/carers Favourable comments from the audience on how the stand sparked their interest. |
Year(s) Of Engagement Activity | 2014,2015 |
URL | http://rhizosphere.org/lab-news/ |
Description | Wymondham academy school visit |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Schools |
Results and Impact | Talk to 6th form students to encourage them to consider science as a career Students keen to apply for university places |
Year(s) Of Engagement Activity | 2014 |