Integrated systems approach for preventing uterine disease in dairy cattle
Lead Research Organisation:
University of Glasgow
Department Name: College of Medical, Veterinary, Life Sci
Abstract
Multi-pathogen infections of the uterus after parturition are endemic causing clinical uterine disease in 40% of dairy cattle and a further 20-40% develop subclinical disease each year. The number of animals requiring treatment is rising as milk production increases. Uterine disease causes infertility, delayed conception, disruption of ovarian cycles, involuntary culling for failure to conceive, and mortality, costing the EU dairy industry EURO1.4 billion/year. The cost to the animal is pain and suffering for several weeks. The cost to the environment is more greenhouse gas emissions, land and water degradation because more cattle have to be kept on farms to replace infertile animals. Research into uterine infection has been neglected compared with other major diseases; there are no vaccines or prevention strategies, and treatment relies on antibiotics and hormones. However, there has been an explosion of knowledge about innate and mucosal immunity in the last 10 years, which provides insights that can be exploited to prevent disease. Furthermore, our recent work has identified several potential strategies to prevent or limit this endemic disease that urgently need examining. Now is the strategic moment where concerted action between the partners is likely to have an impact on uterine disease. This project aims to translate novel strategies into potential products that limit the impact of uterine disease. We will pursue 3 objectives: 1. Refine our underpinning knowledge and tools for postpartum uterine disease in cattle. This includes refining and benchmarking our in vitro and in vivo models of disease; exploring the details of the microbes that infect the uterus, including our newly discovered endometrial pathogenic E. coli (EnPEC); and developing molecular tools to evaluate and diagnose disease. 2. Test candidate strategies to prevent or limit uterine disease using our in vitro and in vivo models. 3. Translate the best candidate strategies that prevent or limit uterine disease to pre-clinical field trials. The project addresses the first translational gap between basic science and the generation of ideas or products for animal health. We are fortunate to have support from our industrial partners, who also have the expertise to then take the results of the project to market for the benefit of all stakeholders.
Technical Summary
Multi-pathogen infections of the uterus after parturition are endemic causing clinical uterine disease in 40% of dairy cattle and a further 20-40% develop subclinical disease each year. The number of animals requiring treatment is rising as milk production increases. Uterine disease causes infertility, delayed conception, disruption of ovarian cycles, involuntary culling for failure to conceive, and mortality, costing the EU dairy industry EURO1.4 billion/year. The cost to the animal is pain and suffering for several weeks. The cost to the environment is more greenhouse gas emissions, land and water degradation because more cattle have to be kept on farms to replace infertile animals. This project aims to translate novel strategies into potential products that limit the impact of uterine disease. We will pursue 3 objectives: 1. Refine our underpinning knowledge and tools for postpartum uterine disease in cattle. 2. Test candidate strategies to prevent or limit uterine disease using our in vitro and in vivo models. 3. Translate the best candidate strategies that prevent or limit uterine disease to pre-clinical field trials.
Planned Impact
AS LEAD ORGANISATION'S DOCUMENT Fit to EMIDA goals 1. Supports EU collaboration at a strategic point in research development for a neglected microbial disease of livestock. 2. Coordinated transnational approach provides a critical research mass to accelerate progress to prevent uterine disease, which costs the EU EURO1.4 billion each year. 3. Reducing the incidence and severity of a common infection by vaccination or regulation of mucosal immunity, will: a. Improve cattle health, fertility and productivity b. Enhance the sustainability of EU dairy farming c. Reduce the environmental footprint of dairy cattle d. Improve animal welfare e. Increase European animal health industry competiveness f. Translate basic science to products that address an endemic disease of livestock g. Develop novel diagnostic tools for subclinical disease h. Reduce the reliance on antimicrobial and hormone treatments 4. Step up durable reproductive disease research in the EU. 5. Bring substantial funding to the EU from Pfizer's group of world-leading pharmaceutical companies.
Publications
Amos MR
(2014)
Differential endometrial cell sensitivity to a cholesterol-dependent cytolysin links Trueperella pyogenes to uterine disease in cattle.
in Biology of reproduction
Goldstone RJ
(2014)
Draft Genome Sequence of Escherichia coli MS499, Isolated from the Infected Uterus of a Postpartum Cow with Metritis.
in Genome announcements
Goldstone RJ
(2014)
Draft Genome Sequence of Trueperella pyogenes, Isolated from the Infected Uterus of a Postpartum Cow with Metritis.
in Genome announcements
Healey G
(2016)
Mevalonate Biosynthesis Intermediates Are Key Regulators of Innate Immunity in Bovine Endometritis
in The Journal of Immunology
Düvel A
(2014)
Peripheral blood leukocytes of cows with subclinical endometritis show an altered cellular composition and gene expression.
in Theriogenology
Hussen J
(2013)
Phenotypic and functional heterogeneity of bovine blood monocytes.
in PloS one
Connolly JP
(2015)
The host metabolite D-serine contributes to bacterial niche specificity through gene selection.
in The ISME journal
Description | Analysis of multiple E. coli strains isolated from cases of bovine metritis has identified gene content potentially contributing to pathogenicity and which could possibly be used towards reducing disease. Similar analyses of Trueperella pyogenes have shown that strains are highly similar in genome content and have identified additional virulence-associated factors. Proteomics analysis for both E. coli and T. pyogenes metritis isolates has defined major protein components. Metabolome analyses have indicated extensive strain- and growth condition-dependent heterogeneity. |
Exploitation Route | The findings will require verification with a larger, more broadly representative panel of metritis bacterial isolates to establish how typifying these observations are, and the extent to which metritis isolates represent a distinct, novel group. Findings to date provide targets which may represent options towards pathogen detection and disease control. |
Sectors | Agriculture, Food and Drink |
Description | The project was a Partnership involving 4 academic and 1 industry partner. Findings were appraised through regular face-to-face meetings but were not prioritised for further development at this time. |
First Year Of Impact | 2012 |
Sector | Agriculture, Food and Drink |
Title | Genome_2 |
Description | Genome sequences for prototypic Escherichia coli isolates from bovine metritis/endometritis. |
Type Of Material | Database/Collection of data |
Year Produced | 2014 |
Provided To Others? | Yes |
Impact | None known besides academic |
URL | http://ftp://ftp.ncbi.nlm.nih.gov/genomes/all/GCF_000701145.1_EcMS4991.0 |
Title | Genomes |
Description | Genome sequences for prototypic Trueperella pyogenes isolates from bovine metritis/endometritis. |
Type Of Material | Database/Collection of data |
Year Produced | 2013 |
Provided To Others? | Yes |
Impact | None known |