Enzyme Cascades and Synthetic Biology Routes to Non-Natural Alkaloids
Lead Research Organisation:
University College London
Department Name: Chemistry
Abstract
Plants and medicinal compounds from plants have been used for centuries to treat pain, infections, asthma, cancer and many of these plant natural products are used in modern medicine, for example: the analgesics morphine and codeine;
anti-malarial quinine for malaria; vinblastine and homoharringtonine for cancer treatment, and ephedrine for asthma. There are over 14,000 natural compounds of the alkaloid family and many possess biological activities. Recently, there has been intensive interest in producing some of these natural compounds using microbes, rather than isolating them from plants in parts of the world that are difficult to access. Synthetic biology approaches to achieve this are proving to be extremely powerful and recent articles have highlighted the possibility of producing morphine from yeast.
In previous work we have investigated some of the early steps and enzymes that use amino acids and perform transformations towards a key intermediate in the pathways that plants use to synthesise the benzylisoquinoline alkaloids, a family of over 2500 alkaloids. We now aim to use these enzymes, together with other enzymes not used in the plant pathway, and new enzymes that have been little explored in biocatalytic applications. The enzymes will be used in a laboratory bacterium to generate a wide range of structurally diverse compounds that are not found in nature. Compounds will screened for antibacterial properties.
Enzymes will be used in in vitro cascades to generate three, four, five, or more multiple enzyme pathways, and also by establishing the pathways in bacteria we will be able to generate new compounds in a reproducible way using a renewable resource.
anti-malarial quinine for malaria; vinblastine and homoharringtonine for cancer treatment, and ephedrine for asthma. There are over 14,000 natural compounds of the alkaloid family and many possess biological activities. Recently, there has been intensive interest in producing some of these natural compounds using microbes, rather than isolating them from plants in parts of the world that are difficult to access. Synthetic biology approaches to achieve this are proving to be extremely powerful and recent articles have highlighted the possibility of producing morphine from yeast.
In previous work we have investigated some of the early steps and enzymes that use amino acids and perform transformations towards a key intermediate in the pathways that plants use to synthesise the benzylisoquinoline alkaloids, a family of over 2500 alkaloids. We now aim to use these enzymes, together with other enzymes not used in the plant pathway, and new enzymes that have been little explored in biocatalytic applications. The enzymes will be used in a laboratory bacterium to generate a wide range of structurally diverse compounds that are not found in nature. Compounds will screened for antibacterial properties.
Enzymes will be used in in vitro cascades to generate three, four, five, or more multiple enzyme pathways, and also by establishing the pathways in bacteria we will be able to generate new compounds in a reproducible way using a renewable resource.
Technical Summary
This project aims to design and assemble de novo pathways in vitro and in vivo for the synthesis of novel complex alkaloids with new functionalities. Notably, while existing approaches using plant enzymes have targeted naturally occuring alkaloids, here the focus is distinctive with the use of non-natural substrates as starting materials to generate diverse non-natural alkaloids.
We will use tyrosine hydroxylases, tyrosine decarboxylases and DOPA decarboxylases previously developed in our laboratory, and new enzymes identified via metagenomics to generate dopamine analogues from non-natural amino acids for use with the enzyme norcoclaurine synthase (NCS). We will also use enzymes including our available transaminases, alcohol dehydrogenases and ene-reductases to generate aldehydes in situ for use in coupling reactions with NCS. The amines and aldehydes will then be used in assays with our library of native and mutant NCSs to generate novel tetrahydroisoquinoline alkaloids (THIAs) that are structurally diverse and have potential for subsequent chemical cyclisation. From this selected reactions will be optimised, and compounds generated will be screened for antibacterial properties.
In preliminary work we have established that some NCSs can accept ketones (non-ketoacids) which is exciting as there are very few routes to 1,1'-substituted THIAs. This route to generate novel THIAs will be explored using a range of ketones together with amines produced using hydroxylases and decarboxylases. Further structural diversity will be incorporated using methyltransferases and a recently developed S-adenosyl methionine cascade. Furthermore we will use C-C phenolic coupling enzymes to mediate further ring forming reactions to produce for example novel aporphine alkaloids.
Using this toolbox of enzymes we will then assemble in vitro reaction cascades to and construct an in vivo pathway to build routes to structurally diverse novel alkaloids.
We will use tyrosine hydroxylases, tyrosine decarboxylases and DOPA decarboxylases previously developed in our laboratory, and new enzymes identified via metagenomics to generate dopamine analogues from non-natural amino acids for use with the enzyme norcoclaurine synthase (NCS). We will also use enzymes including our available transaminases, alcohol dehydrogenases and ene-reductases to generate aldehydes in situ for use in coupling reactions with NCS. The amines and aldehydes will then be used in assays with our library of native and mutant NCSs to generate novel tetrahydroisoquinoline alkaloids (THIAs) that are structurally diverse and have potential for subsequent chemical cyclisation. From this selected reactions will be optimised, and compounds generated will be screened for antibacterial properties.
In preliminary work we have established that some NCSs can accept ketones (non-ketoacids) which is exciting as there are very few routes to 1,1'-substituted THIAs. This route to generate novel THIAs will be explored using a range of ketones together with amines produced using hydroxylases and decarboxylases. Further structural diversity will be incorporated using methyltransferases and a recently developed S-adenosyl methionine cascade. Furthermore we will use C-C phenolic coupling enzymes to mediate further ring forming reactions to produce for example novel aporphine alkaloids.
Using this toolbox of enzymes we will then assemble in vitro reaction cascades to and construct an in vivo pathway to build routes to structurally diverse novel alkaloids.
Planned Impact
The project will have impact in each of the areas defined by the BBSRC and RCUK: economy, society, knowledge and people.
In terms of economic impact the PI and CoI have experience of working with industry, and the commercialisation of research findings and creation of a spin-out company. The enzymes and data resulting from this project will be made available (after IP protection) to the wider academic and industrial community via publication, and dissemination at conferences and meetings for example the BBSRC NIBB Networks. Furthermore, the novel alkaloids will be screened for antibacterial properties, which may identify new bioactive compounds. Beneficiaries of this work therefore extends beyond the immediate participants. It will benefit the UK economy by sustaining high-level research and the successfully developed biocatalytic cascades and pathways will be promoted widely within the pharmaceutical and fine chemical industries.
The impact of knowledge generation from the research will arise from the communication and publication of new scientific advances and public outreach activities to schools, detailed in the pathways to impact.
The societal impact will arise from the development of available and new enzymes in multienzyme systems to generate new alkaloids. The use of biocatalysis in the chemical and pharmaceutical industry is growing, but is limited by the types of enzymes used and the compounds generated. By identifying new enzymes and methods for assembling these to achieve sustainable synthesis, these resources will assist the UK government meet its targets on greenhouse gas emissions and help mitigate the negative environmental effects of global warming. The use of synthetic biology for pathway construction is a key part of the cross council research strategy and synthetic biology is one of the BBSRC strategic priority areas. In addition, if new biologically active compounds are identified that can be made sustainably and cheaply this could impact positively on the general public.
The impact on people will arise from training and career development of the PDRA and provision of skilled scientist in the industrial biotechnology sector.
The findings will also influence future research directions, since the successful completion of this proposal will provide the data to embed biocatalytic strategies to new alkaloids that has to date been little unexplored. In addition, a wide spectrum of potential end-users, which include commercial companies, charities and the health care systems may profit from the outputs of this project in the longer term. While the impact of the funded research will be at an international level, the intellectual property and thus the commercial profits will be likely to enhance the competiveness of the UK. UCL who will hold the intellectual property rights to the enzymes and compounds generated in this proposal, will benefit from licensing and industry collaboration.
In terms of economic impact the PI and CoI have experience of working with industry, and the commercialisation of research findings and creation of a spin-out company. The enzymes and data resulting from this project will be made available (after IP protection) to the wider academic and industrial community via publication, and dissemination at conferences and meetings for example the BBSRC NIBB Networks. Furthermore, the novel alkaloids will be screened for antibacterial properties, which may identify new bioactive compounds. Beneficiaries of this work therefore extends beyond the immediate participants. It will benefit the UK economy by sustaining high-level research and the successfully developed biocatalytic cascades and pathways will be promoted widely within the pharmaceutical and fine chemical industries.
The impact of knowledge generation from the research will arise from the communication and publication of new scientific advances and public outreach activities to schools, detailed in the pathways to impact.
The societal impact will arise from the development of available and new enzymes in multienzyme systems to generate new alkaloids. The use of biocatalysis in the chemical and pharmaceutical industry is growing, but is limited by the types of enzymes used and the compounds generated. By identifying new enzymes and methods for assembling these to achieve sustainable synthesis, these resources will assist the UK government meet its targets on greenhouse gas emissions and help mitigate the negative environmental effects of global warming. The use of synthetic biology for pathway construction is a key part of the cross council research strategy and synthetic biology is one of the BBSRC strategic priority areas. In addition, if new biologically active compounds are identified that can be made sustainably and cheaply this could impact positively on the general public.
The impact on people will arise from training and career development of the PDRA and provision of skilled scientist in the industrial biotechnology sector.
The findings will also influence future research directions, since the successful completion of this proposal will provide the data to embed biocatalytic strategies to new alkaloids that has to date been little unexplored. In addition, a wide spectrum of potential end-users, which include commercial companies, charities and the health care systems may profit from the outputs of this project in the longer term. While the impact of the funded research will be at an international level, the intellectual property and thus the commercial profits will be likely to enhance the competiveness of the UK. UCL who will hold the intellectual property rights to the enzymes and compounds generated in this proposal, will benefit from licensing and industry collaboration.
Organisations
People |
ORCID iD |
Helen Hailes (Principal Investigator) | |
John Ward (Co-Investigator) |
Publications
Zhao J
(2019)
Biomimetic Phosphate-Catalyzed Pictet-Spengler Reaction for the Synthesis of 1,1'-Disubstituted and Spiro-Tetrahydroisoquinoline Alkaloids.
in The Journal of organic chemistry
Yu H
(2020)
Engineering transketolase to accept both unnatural donor and acceptor substrates and produce a-hydroxyketones.
in The FEBS journal
Dobrijevic D
(2019)
Metagenomic ene-reductases for the bioreduction of sterically challenging enones.
in RSC advances
Roddan R
(2020)
Single step syntheses of (1S)-aryl-tetrahydroisoquinolines by norcoclaurine synthases
in Communications Chemistry
Lechner H
(2018)
Library of Norcoclaurine Synthases and Their Immobilization for Biocatalytic Transformations.
in Biotechnology journal
Dobrijevic D
(2020)
pET expression vector customized for efficient seamless cloning.
in BioTechniques
Linke JA
(2023)
Production of indigo by recombinant bacteria.
in Bioresources and bioprocessing
Hickman TWP
(2020)
Characterisation of four hotdog-fold thioesterases for their implementation in a novel organic acid production system.
in Applied microbiology and biotechnology
Wang Y
(2019)
Design and Use of de novo Cascades for the Biosynthesis of New Benzylisoquinoline Alkaloids.
in Angewandte Chemie (International ed. in English)
Description | New enzymes for use in enzymatic cascades to alkaloids. These include new tyrosinase and decarboxylase enzymes and the incorporation of these into enzyme cascades to give novel alkaloids in very high yields and excellent product selectivities. These have been sent for screening against dopamine receptors, and as anti-malarial and anti- bacterial compounds. We have also constructed cascades using other enzymes to make aldehyde or ketone substrates such as alcohol dehydrogenases, transaminases and ene-reductases. Moreover we are investigating the combination of enzymatic and chemical cascades to enhance the utility of this approach. Finally we have generated P450s that have also been screened against substrates. Furthermore we have expanded the substrates accepted by the enzyme norcoclaurine synthases to give many novel tetrahydroisoquinoline products that can not be generated using traditional chemical synthetic methods. |
Exploitation Route | These enzymes can be utilised by others for a range of other applications. We have published 9 papers arising from this grant and have 2 further papers to submit in the near future. |
Sectors | Chemicals,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
Description | We have discussed mutagenesis of the enzymes with a potential commercial partner, and again have discussed this at a recent comference. |
First Year Of Impact | 2018 |
Sector | Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
Description | 17-ERACoBioTech Methyl Transferases for the Functional Diversification of Bioactives: BioDiMet |
Amount | £1,911,000 (GBP) |
Funding ID | BB/R021643/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 03/2018 |
End | 04/2022 |
Description | H2020 956631 MSCA ITN 2020 CC-TOP |
Amount | € 3,971,466 (EUR) |
Funding ID | 956631 |
Organisation | European Commission H2020 |
Sector | Public |
Country | Belgium |
Start | 03/2021 |
End | 02/2025 |
Description | A research talk entitled 'NORCOCLAURIN SINTASA, UN BIOCATALIZADOR PARA LA SÍNTESIS DE ALCALOIDES BENZOISOQUINOLÍNICOS', at JEB 2018, Oviedo-Spain, June 25-26, 2018 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | A research talk entitled 'NORCOCLAURIN SINTASA, UN BIOCATALIZADOR PARA LA SÍNTESIS DE ALCALOIDES BENZOISOQUINOLÍNICOS', JEB 2018, Oviedo-Spain, June 25-26, 2018. This talk was given by Daniel mendez-Sanchez and also provided an opportunity for networking. |
Year(s) Of Engagement Activity | 2018 |
Description | An invited talk entitled 'Green Chemistry: Enzymatic and Chemoenzymatic Strategies in Synthesis', at the 10th Choshu-London Symposium, Yamaguchi University, Japan, on 15th March 2018. |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Part of the Tohoku Organic Chemistry Symposium, Tohoku University, Sendai, Japan, on 12th March 2018 |
Year(s) Of Engagement Activity | 2018 |
Description | An invited talk entitled 'Green Chemistry: Enzymatic and Chemoenzymatic Strategies in Synthesis', at the Tohoku Organic Chemistry Symposium, Tohoku University, Sendai, Japan, on 12th March 2018 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | A short lecture talk in Japan to enhance collaborations. |
Year(s) Of Engagement Activity | 2018 |
Description | Conference (on-line) talk |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Presentation given by Fabiana Subrizi entitled 'Multienzyme one-pot cascades incorporating methyl transferases for the strategic diversification of THIQ alkaloids' at NextGenBiocatalysis, 12th February 2021 |
Year(s) Of Engagement Activity | 2021 |
Description | Conference poster 'NORCOCLAURINE SYNTHASE A USEFUL TOOL TO BENZOISOQUINOLINE ALKALOIDS, Biotrans Budapest Hungary, July 9-13, 2017 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Poster presentation entitled 'NORCOCLAURINE SYNTHASE A USEFUL TOOL TO BENZOISOQUINOLINE ALKALOIDS', Biotrans Budapest Hungary, July 9-13, 2017. This was presented by Daniel Mendez-Sanchez |
Year(s) Of Engagement Activity | 2017 |
Description | Conference presentation entitled 'Biocatalytic Routes to Amines' at the Catalysis Hub |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | To highlight to the catalysis community how amines, including alkaloids, can be formed using biocatalysts |
Year(s) Of Engagement Activity | 2018 |
URL | https://www.rc-harwell.ac.uk/uk-catalysis-hub-summer-conference-2018 |
Description | Departmental seminar at Oxford entitled 'The use of enzymes for C-C bond formation and amine synthesis' |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Departmental seminar to PDRAs, lecturers, postgraduates, undergraduates |
Year(s) Of Engagement Activity | 2018 |
Description | Departmental talk at the Technical University of Delft on 16th April 2018 entitled 'Biocatalytic routes to tetrahydroisoquinoline alkaloids' |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | To introduce how reaction cascades can be constructed to alkaloids |
Year(s) Of Engagement Activity | 2018 |
Description | Gordon Research Conference- Invited seminar |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Talk entitled 'Norcoclaurine Synthases and Enzymatic and Chemoenzymatic Cascades' |
Year(s) Of Engagement Activity | 2018 |
URL | https://www.grc.org/biocatalysis-conference/2018/ |
Description | Industrial Synthetic Biology Congress 2018 - invited talk entitled 'Synthesising Chemicals Using Synthetic Pathways In Plants' |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Industry/Business |
Results and Impact | Highlighting biological routes to making complex alkaloids |
Year(s) Of Engagement Activity | 2018 |
URL | https://www.oxfordglobal.co.uk/industrialsyntheticbiology-congress/ |
Description | Innovation & Sustainability Chemistry Consortium invited talk on Zoom |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Invited talk entitled 'Strategies for the Sustainable Synthesis of Chiral Compounds through Biocatalysis' at India-UK ISCC: Innovation & Sustainability Chemistry Consortium, 20th April 2021 (on zoom). |
Year(s) Of Engagement Activity | 2021 |
Description | Invited lecture at Protein Engineering CONGRESS EU in Frankfurt |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Industry/Business |
Results and Impact | Invited lecture entitled 'Synthesising Chiral Compounds by Leveraging Sustainable Biocatalysts' at Protein Engineering CONGRESS EU in Frankfurt on 12-13th November 2019 |
Year(s) Of Engagement Activity | 2019 |
Description | Invited lecture at international conference |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Invited lecture on 'Enzymatic synthesis of novel halogenated alkaloids using a 'parallel cascade' strategy in vitro', at BIOTRANS I GRAZ 2021 on July 18th 2021 (on zoom). |
Year(s) Of Engagement Activity | 2021 |
Description | Invited lecture at the '28th Annual Symposium: The Synthesis and Applications of Isotopically Labelled Compounds in Cambridge' on 8th November 2019 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | Invited lecture at entitled 'Enzymatic Approaches to Natural and Non-Natural Alkaloids' |
Year(s) Of Engagement Activity | 2019 |
Description | Invited lecture at the European Symposium of Bio-organic Chemistry (ESBOC) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Invited lecture at the European Symposium of Bio-organic Chemistry (ESBOC), 'Enzymatic single step reactions and the construction of biocatalytic cascades to alkaloids', Wales, 22nd May 2022. Lots of discussion and questions afterwards. |
Year(s) Of Engagement Activity | 2022 |
URL | https://www.esboc.org.uk/?page_id=768 |
Description | Invited lecture at the Gordon Research Conference on Synthetic Biology held in Waterville Valley USA |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Invited lecture entitled 'Enzymatic approaches to natural and non-natural alkaloids' at the Gordon Research Conference on Synthetic Biology held in Waterville Valley USA, on 16th July 2019. There followed interesting discussion on building pathways. While at the conference I also presented the GRC 'Power Hour' which aims to discuss issues related to equality and diversity. |
Year(s) Of Engagement Activity | 2019 |
URL | https://www.grc.org/synthetic-biology-conference/2019/ |
Description | Invited lecture at the Symposium on Biocatalysis & Biomass, QUB |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | Invited lecture at the Symposium on Biocatalysis & Biomass, QUB, 19th July 2022 'The biocatalytic synthesis of amines'. Discussions afterwards. |
Year(s) Of Engagement Activity | 2022 |
Description | Invited seminar at a University |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | Invited seminar entitled 'Biocatalytic amine synthesis: from single step reactions to enzyme cascades' 17th May 2021 at Cardiff School of Chemistry (on zoom). |
Year(s) Of Engagement Activity | 2021 |
Description | Invited seminar in the Dept Chemistry, Bristol University |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | Invited seminar in the Dept Chemistry, Bristol University 'Applications of biocatalysts for bond making and bond breaking', 1st March 2023. Extensives disciussion after. |
Year(s) Of Engagement Activity | 2023 |
Description | Invited speaker at Biocascades Symposium 2018, Bielefeld2018, entitled 'Enzyme cascades to tetrahydroisoquinolines using norcoclaurine synthases' on 10th April 2018 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | The conferences covered biocatalytic cascades with a mixture of industrial and academic speakers |
Year(s) Of Engagement Activity | 2018 |
Description | Invited talk at AmineBiocat4.0 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Industry/Business |
Results and Impact | Talk entitled 'Biocatalytic amine synthesis: from single step reactions to enzyme cascades,', on 17th February 2020. After, I discussed opportunities to collaborate with european researchers in the field. |
Year(s) Of Engagement Activity | 2020 |
URL | https://aminebiocat4.com/2019/06/27/welcome-to-amine-biocatalysis-4-0/ |
Description | Invited talk at the London Synthetic Biology Showcase 2019 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Professional Practitioners |
Results and Impact | The PDRA gave an invited talk entitled 'Enzymatic methylation cascades for the synthesis of tetrahydroisoquinoline alkaloids' at the London Synthetic Biology Showcase 2019 on 23rd July 2019. There was discussion afterwards on the applications of this approach. |
Year(s) Of Engagement Activity | 2019 |
URL | https://www.evensi.uk/london-synthetic-biology-showcase-2019-27-torrington-square/316243888 |
Description | Inward mission on industrial biotechnology: 12 Feb 2018 |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | Indian Industrial Biotech delegation at the UCL and will lead to some connections towards UK-India partnerships in future. Attendees · Dr. Annamma Anil Odaneth: Assistant Professor, Department of Biotechnology DBT- Institute of Chemical Technologies - Centre for Energy Biosciences, Institute of Chemical Technology, Mumbai, India: E: a.dbtceb@gmail.com · Dr Pramod Wangikar: Professor, Department of Chemical Engineering, Wadhwani Research Center for Bioengineering, Indian Institute of Technology Bombay Mumbai , India: E: wangikar@iitb.ac.in · Dr Rajkumar Rajagopal: Founder & Managing Director, Cellzyme Biotech India Pvt Ltd, Coimbatore, India: E: rajkumar77@gmail.com · Dr Syed Shams Yazdani: Group Leader, Microbial Engineering, Group Coordinator, DBT-ICGEB Centre for Advanced Bio-Energy Research, International Centre for Genetic Engineering and Biotechnology, ICGEB, New Delhi : E: ssyazdani@gmail.com |
Year(s) Of Engagement Activity | 2018 |
Description | Plenary Lecture at Biotrans |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Plenary talk entitled 'Biocatalytic Single-Step Reactions and Enzyme Cascades' |
Year(s) Of Engagement Activity | 2019 |
URL | https://biotrans2019.com/ |
Description | Plenary lecture Firbush |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Professional Practitioners |
Results and Impact | Talk entiled 'Biocatalytic amine synthesis and C-C bond formation: from single step reactions to enzyme cascades' at the 18th RSC Chemical Biology and Bioorganic Chemistry Firbush Symposium on the 6th September 2019 |
Year(s) Of Engagement Activity | 2019 |
URL | https://www.rsc.org/events/detail/39563/the-18th-rsc-chemical-biology-and-bioorganic-group-cbbg-firb... |
Description | Poster entitled 'NORCOCLAURINE SYNTHASE: A USEFUL TOOL WHICH ALLOWS ACCESS TO TETRAHYDROISOQUINOLINE ALKALOIDS', Gordon's conference, Biddeford, Maine, July 8 - 13, 2018 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | A poster entitled 'NORCOCLAURINE SYNTHASE: A USEFUL TOOL WHICH ALLOWS ACCESS TO TETRAHYDROISOQUINOLINE ALKALOIDS' at the Gordon's seminar, Biddeford, Maine, July 7-8, 2018, and at Gordon's conference, Biddeford, Maine, July 8 - 13, 2018. A number of discussions arose including potential collaborations and the poster was presented by Daniel Mendez-Sanchez |
Year(s) Of Engagement Activity | 2018 |
URL | https://www.grc.org/biocatalysis-conference/2018/ |
Description | Royal Society of Chemistry sponsored seminar at the Department of Biosciences, Exeter |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Undergraduate students |
Results and Impact | An invited RSC sponsored seminar entitled 'Biocatalytic Strategies to Isoquinoline Alkaloids' at the Department of Biosciences, Exeter. Discussion with undergraduates after the talk about using biocatalysts to make pharmaceuticals |
Year(s) Of Engagement Activity | 2017 |
Description | Seminar at Leeds |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Postgraduate students |
Results and Impact | A talk entitles 'Sustainable synthesis using enzymes: from discovery to applications' to undergraduates and postgraduates |
Year(s) Of Engagement Activity | 2019 |
Description | Talk at an international conference |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | A talk entitled 'Biocatalytic routes to tetraisoquinoline alkaloids using norcoclaurine synthases', at Biotrans2017, Budapest, Hungary |
Year(s) Of Engagement Activity | 2017 |
Description | University visit (Warwick) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Undergraduate students |
Results and Impact | Seminat at Warwick University to undergraduates and postgraduates entitled 'The use of enzymes for C-C bond formation and amine synthesis' describing the results arising from this project |
Year(s) Of Engagement Activity | 2017 |
Description | Webinar presentation |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | Webinar presetation entitled 'The Strategic Diversification of Bioactive Tetrahydroisoquinolines using Methyltransferases', 18th February 2021 at a Webinar on Catalysts|Methyltransferases, Understanding and Application. |
Year(s) Of Engagement Activity | 2021 |