Phospholipase D signalling
Lead Research Organisation:
Babraham Institute
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Cells communicate through the activation of cell surface receptors (a protein that binds to specific molecules). This communication regulates processes such as cell division, survival, migration, differentiation (specialisation), metabolism and contraction. These diverse processes are regulated by a number of signalling pathways in the cell. Considerable research has identified many of these pathways, however, their regulation and the detail of how they control physiological processes remains sketchy. In this project we focus upon pathways regulating the generation of the lipid (fat) messengers phosphatidic acid and lysophosphatidic acid and upon their molecular targets.
Planned Impact
unavailable
Organisations
People |
ORCID iD |
| Michael Wakelam (Principal Investigator) |
Publications
Lindhurst MJ
(2012)
Mosaic overgrowth with fibroadipose hyperplasia is caused by somatic activating mutations in PIK3CA.
in Nature genetics
McDermott MI
(2020)
Mammalian phospholipase D: Function, and therapeutics.
in Progress in lipid research
Norton LJ
(2011)
PLD1 rather than PLD2 regulates phorbol-ester-, adhesion-dependent and Fc{gamma}-receptor-stimulated ROS production in neutrophils.
in Journal of cell science
O'Donnell VB
(2018)
Phospholipid signaling in innate immune cells.
in The Journal of clinical investigation
Pal A
(2012)
PTEN mutations as a cause of constitutive insulin sensitivity and obesity.
in The New England journal of medicine
Rainero E
(2012)
Diacylglycerol kinase a controls RCP-dependent integrin trafficking to promote invasive migration.
in The Journal of cell biology
Rudge SA
(2009)
Inter-regulatory dynamics of phospholipase D and the actin cytoskeleton.
in Biochimica et biophysica acta
Vermeren M
(2016)
The Phospholipase D2 Knock Out Mouse Has Ectopic Purkinje Cells and Suffers from Early Adult-Onset Anosmia
in PLOS ONE
| Description | The study is showing how PLD signalling and PI3kinase signalling cross talk. The importance through the use of KO mice of PLD in controlling the neutrophil superoxide response and the importance of PLD in the ageing brain are being determined. The use of lipidomics technologies are defining the PLD-regulated changes. We have uncovered distinct roles for PLD1 and PLD2 in regulating weight gain and fat metabolism under normal and high fat diet conditions. |
| Exploitation Route | understanding how PLD is involved in lipid metabolism may have an input into understanding steatosis |
| Sectors | Chemicals Pharmaceuticals and Medical Biotechnology |
| Title | PLD2 KO mouse |
| Description | transgenic mouse |
| Type Of Material | Model of mechanisms or symptoms - mammalian in vivo |
| Year Produced | 2012 |
| Provided To Others? | No |
| Impact | none yet |
| Description | Invited lecturer to international meetings (average 2-3 per year) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Generated discussion, exchange of ideas and collaborations |
| Year(s) Of Engagement Activity | Pre-2006,2006,2007,2008,2009,2010,2011,2012,2013,2014,2015,2016,2017,2018,2019 |
| Description | Schools day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | 125 pupils attended schools day, talks given to all and lab practicals ran for two small groups. |
| Year(s) Of Engagement Activity | 2018,2019 |