Systematic characterisation of epigenomes and transcriptomes during early mouse development
Lead Research Organisation:
Babraham Institute
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Work in this Theme provides an unprecedented opportunity for this grouping of PIs and for the national and international scientific community to establish reference epigenomes for the key stages of epigenetic reprogramming and programming in germ cells, preimplantation embryos, and lineage commitment in early postimplantation embryos. This resource together with the development of an integrated epigenomics database will underpin the biological exploration of epigenetic reprogramming mechanisms, the unravelling of signalling principles that pattern the epigenome during development and ageing, and the understanding of how non-coding RNAs regulate the epigenome. This resource will become available to the national and international community through established high quality links (eg EU Network of Excellence, IHEC) and will be of great value not only for the epigenetics community, but also for developmental biologists, geneticists, reproductive and evolutionary biologists, stem cell researchers, and those working on the biology of ageing
Planned Impact
unavailable
Organisations
- Babraham Institute (Lead Research Organisation)
- Cancer Research Technology (CRT) (Collaboration)
- Sapienza University of Rome (Collaboration)
- National Institute of Environmental Health Sciences (Collaboration)
- University of Cambridge (Collaboration)
- MERCK (Collaboration)
- Broad Institute (Collaboration)
- Active Motif (Collaboration)
- Technical University of Dresden (Collaboration)
- University of Texas (Collaboration)
- Cambridge Epigenetix (Collaboration)
- Helmholtz Zentrum München (Collaboration)
- Medical Research Council (MRC) (Collaboration)
- University of Edinburgh (Collaboration)
Publications
Angermueller C
(2016)
Parallel single-cell sequencing links transcriptional and epigenetic heterogeneity
in Nature Methods
Arand J
(2015)
Selective impairment of methylation maintenance is the major cause of DNA methylation reprogramming in the early embryo.
in Epigenetics & chromatin
Branco MR
(2016)
Maternal DNA Methylation Regulates Early Trophoblast Development.
in Developmental cell
Brighton PJ
(2017)
Clearance of senescent decidual cells by uterine natural killer cells in cycling human endometrium.
in eLife
Chandra T
(2015)
Global reorganization of the nuclear landscape in senescent cells.
in Cell reports
Chrysanthou S
(2018)
A Critical Role of TET1/2 Proteins in Cell-Cycle Progression of Trophoblast Stem Cells.
in Stem cell reports
Clark SJ
(2017)
Genome-wide base-resolution mapping of DNA methylation in single cells using single-cell bisulfite sequencing (scBS-seq).
in Nature protocols
Clark SJ
(2016)
Single-cell epigenomics: powerful new methods for understanding gene regulation and cell identity.
in Genome biology
Collier AJ
(2017)
Comprehensive Cell Surface Protein Profiling Identifies Specific Markers of Human Naive and Primed Pluripotent States.
in Cell stem cell
Eckersley-Maslin MA
(2018)
Dynamics of the epigenetic landscape during the maternal-to-zygotic transition.
in Nature reviews. Molecular cell biology
| Description | This research theme aimed at mapping epigenetic information across the genome during key stages in the mammalian life-course, in particular the specification and development of germ cells and in the early embryo, during which major changes in epigenetic states occur and are necessary for cells to change from a germ cell fate to becoming totipotent, and then again to allow embryonic lineages to arise. Many epigenomes were determined across these stages, strongly enriching our knowledge in this important area for development and transgenerational epigenetic inheritance. We have pioneered low and single cell technologies, allowing studies of the transcriptome, methylome and chromatin heterogeneity, as well as highly-sensitive methods for detecting derivatives of 5-methylcystosine. These methods are proving transformative in scientific areas as diverse as oocyte epigenetics (where material is highly limited and improving the sensitivity of methods substantially reduces animal requirements), determination of lineage specificity during embryogenesis, and for investigating epigenetic heterogeneity in ageing. |
| Exploitation Route | These findings have been taken up by the community as judged by research publications that build on our results |
| Sectors | Healthcare Pharmaceuticals and Medical Biotechnology |
| URL | https://www.babraham.ac.uk/our-research/epigenetics |
| Description | As part of the wider Babraham Institute strategy, across the course of this ISP we have developed a much broader public engagement strategy, undertaking large prestigious events including two Royal Society exhibits specifically showcasing our work (Epigenetics: DNA is not your destiny and Race Against the Ageing Clock), as well as presentations at local science fairs and engagement with local schools. We have also contributed substantially to transferable skills development, analytical thinking and training, mentoring and career development. |
| First Year Of Impact | 2012 |
| Sector | Education,Healthcare |
| Impact Types | Cultural Societal Economic |
| Description | Babraham Institute Public Dialogue 2015 |
| Geographic Reach | National |
| Policy Influence Type | Citation in other policy documents |
| Impact | Improved public understanding of biological sciences and the work of the Babraham Institute |
| URL | http://www.babraham.ac.uk/get-involved/partnerships-page/public-dialogue |
| Description | CTR Board of Managers (MH) |
| Geographic Reach | Europe |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Impact | Member, Board of Managers of the Centre for Trophoblast Research, Cambridge assessing funding for targetted PhD studentship and Next Generation fellowship applciations and influencing science strategy within this research area. |
| Description | H2020 EU-LIFE project "LIBRA", co-lead work package "Gender Equality in Research" |
| Geographic Reach | Europe |
| Policy Influence Type | Influenced training of practitioners or researchers |
| Impact | Consideration of gender effects in research, appreciation that there may be sex differences in cell lines, animal models and treatment regimes that ought to be taken into consideration when designing studies. |
| Description | Review panel member, DFG Clinical Research Unit (Germany) (MH) |
| Geographic Reach | Europe |
| Policy Influence Type | Membership of a guideline committee |
| Impact | Funding assessment for a major research unit on male infertility which includes improvement of patient treatment options and better patient assessment and stratification. Funding was recommended by teh panel and was implemented. |
| Description | Wellcome Trust funding commitee |
| Geographic Reach | National |
| Policy Influence Type | Participation in a guidance/advisory committee |
| Description | EU BLUEPRINT |
| Amount | £500,000 (GBP) |
| Funding ID | 282510 |
| Organisation | European Commission |
| Department | Seventh Framework Programme (FP7) |
| Sector | Public |
| Country | European Union (EU) |
| Start | |
| Description | EU NoE EpiGeneSys |
| Amount | £400,000 (GBP) |
| Funding ID | 257082 |
| Organisation | European Commission |
| Sector | Public |
| Country | Belgium |
| Start | |
| Description | Epigenome patterning in oocytes and its legacies in the embryo |
| Amount | £1,964,775 (GBP) |
| Funding ID | MR/S000437/1 |
| Organisation | Medical Research Council (MRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 07/2018 |
| End | 07/2025 |
| Description | MRC Collaboration Grant (deep sequencing in Epigenomics) |
| Amount | £960,000 (GBP) |
| Funding ID | G0801156 |
| Organisation | Medical Research Council (MRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | |
| Title | High through-put single-cell DNA methylation profiling |
| Description | We have improved our previously published method of profiling DNA methylation genome-wide in single cells (Smallwood et al., Nat. Methods 2014), by implementing the method on a liquid handing robot (Clark et al. Nat. Protocols 2017), which is allowing much higher through-put applications. An extensive protocol is provided. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | Allowing much higher through-put applications. |
| Title | Single cell bisulfite sequencing |
| Description | Single cell method for methylome sequencing |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2014 |
| Provided To Others? | Yes |
| Impact | Widely quoted technique which is being implemented by other laboratories |
| URL | http://www.nature.com/nmeth/journal/v11/n8/full/nmeth.3035.html |
| Title | Single cell combined RNA and bisulfite sequencing |
| Description | Method for sequencing of the transcriptome and methylome from the same single cell |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2016 |
| Provided To Others? | Yes |
| Impact | This has just been published |
| URL | http://www.nature.com/nmeth/journal/v13/n3/full/nmeth.3728.html |
| Title | Single cell triple-omics |
| Description | Single cell triple-omics combining transcriptome, methylome, and chromatin accessibility from the same single cell. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | This method was just published. |
| Title | Single-cell BS-seq |
| Description | We developed a method to profile DNA methylation genome-wide in single cells, which has now been published (Smallwood et al., Nat. Methods 2014). This capability could have widespread application in assessing DNA methylation in rare cells (e.g., human gametes, preimplantation embryos), to assess DNA methylation heterogeneity amongst cells, which could be an important mechanism in developmental decisions, aging and pathological states, notably in cancer. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2014 |
| Provided To Others? | Yes |
| Impact | The development of this method has led to the establishment of new collaborations for our group. It has also been a key component underpinning the award of a Wellcome Trust Strategic Award by a consortium headed by my colleague Prof. W. Reik. |
| Title | ChIP-seq profiles for Tet1 in TSCs (GSE109545) |
| Description | Chromatin binding profiles of Tet1 protein in trophoblast stem cells (TSCs) |
| Type Of Material | Database/Collection of data |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | Data allow to determine the functional role of Tet1 in TSCs |
| Title | Chromatin modifications during mouse oogenesis |
| Description | High quality native ChIP-seq datasets for histone modifications H3K4me3, H3K27ac, H3K27me3 during oocyte growth and fully grown oocytes in the mouse. ChIP-seq datasets for H3K4me3 in oocytes genetically deficient in DNA methylation (Dnmt3a/Dnmt3b nulls). ChIP-seq datasets for oocytes genetically deficient in the major H3K4 methyltransferase MLL2 (KMT2B). Published in Hanna et al. Nat. Struct. Mol. Biol. 2018 |
| Type Of Material | Database/Collection of data |
| Year Produced | 2018 |
| Provided To Others? | Yes |
| Impact | Recently released datasets, too early to identify impact outside of research group. |
| URL | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE93941GSE93941 |
| Title | DNA methylation and gene expression datasets from cow and pig gametes and embryos. |
| Description | Whole-genome bisulphite-sequencing and RNA-sequencing datasets from porcine and bovine oocytes, sperm and preimplantation embryo stages. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | Too early to say |
| URL | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE143850 |
| Title | DNA methylation during mouse oogenesis |
| Description | Genome-wide DNA methylation datasets by RRBS and PBAT from size-selected mouse oocytes during the oocyte growth phase. Published in Gahurova et al. 2017 Epigenetics and Chromatin. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | Only recently released, impact outside of research group too early to say. |
| URL | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE86297 |
| Title | Epigenomic analysis of DNMT3A mutation |
| Description | DNA methylation, gene expression and histone modification datasets from embryo and adult tissues from mouse strain carrying a point mutation in DNMT3A, which is associated with postnatal growth restriction. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | An analogous mutation in human DNMT3A has been identified to cause extreme primordial growth restriction and similar epigenetic changes. Our data published in Sendžikaite et al Nat Comms 2019 PMID: 31015495. |
| URL | http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE117728 |
| Title | Epigenomic analysis of mouse post-implantation embryos |
| Description | Allellic DNA methylation, gene expression and histone modification datasets from embryonic tissues from mouse. Allows genome-wide identification of genes controlled by genomic imprinting, and distinction of imprinting conferred by DNA methylation or repressive chromatin in oocytes. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | Published in Hanna et al. Genome Biol 2019 PMID: 31665063 |
| URL | http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE124216 |
| Title | Epigenomic analysis of oocytes lacking H3K4 methyltransferase SETD1B |
| Description | H3K4me3 ChIP-seq and DNA methylation (WGBS) datasets from wild-type and Setd1b conditional knock-out mouse oocytes |
| Type Of Material | Database/Collection of data |
| Year Produced | 2022 |
| Provided To Others? | Yes |
| Impact | Too early to say |
| URL | http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE167987 |
| Title | Gene expression during mouse oogenesis |
| Description | Deeply sequenced RNA-seq datasets from size-selected mouse oocytes during the oocyte growth phase. Published in Gahurova et al. 2017 Epigenetics and Chromatin. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | Only recently released, too early to identify impacts beyond research group. |
| URL | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE86297 |
| Title | Oocyte ChIP-seq datasets |
| Description | ChIP-seq datasets for selected histone modifications in mouse growing oocytes. DNA methylation profiles of oocytes lacking the histone demethylases KDM1A, KDM1B. As reported in Stewart et al., 2015 Genes and Development, 29, 2449-2462 |
| Type Of Material | Database/Collection of data |
| Year Produced | 2015 |
| Provided To Others? | Yes |
| Impact | Too soon to define notable impacts, as the associated manuscript was published in Genes and Development in December 2015 |
| URL | http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE74549 |
| Title | Oocyte transcriptome, mouse |
| Description | Deep strand-specific RNA-seq to establish in-depth transcriptome of mouse oocytes, as reported in Veselovska et al., 2015 Genome Biology 16, 209 |
| Type Of Material | Database/Collection of data |
| Year Produced | 2015 |
| Provided To Others? | Yes |
| Impact | The publication in Genome Biology associated with these datasets has already attracted >30 citations, suggesting a good level of uptake of the data by the research community. |
| URL | http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE70116 |
| Title | Stem Cell methylomes |
| Description | DNA methylation (meDIP)-seq profiling of ESCs, TSCs, XEN cells and EpiSCs |
| Type Of Material | Database/Collection of data |
| Provided To Others? | No |
| Impact | Data were deposited in publicly accessible databases. Data will be useful to many other researchers for meta-analyses. |
| Title | Transcriptome datasets of reproductive tissues in ageing females (GSE98901) |
| Description | RNA-seq datasets of trophoblast, endometrium and deciduae of mouse conceptuses developed in young versus aged females |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | Significant changes imposed by maternal age on expression profiles of endoemtrial tissue and decidual development. Work shows the impact of the older female on reproductive success, unrelated to oocyte fitness. |
| Title | Webtool for integration of high-throughput datasets and their rapid analysis |
| Description | Generation of a webtool allowing rapid data analysis of high-througput sequencing datasets. |
| Type Of Material | Computer model/algorithm |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | Webtool allows to upload and integrate multiple datasets of different nature (such as ChIP-seq, RNA-seq, DNA methyaltion profiles) and allows to perform rapid multivariate analyses on them to find co-occpuancy, mutual exclusion etc |
| Description | Active Motif DNA modification antibody |
| Organisation | Active Motif |
| Country | United States |
| Sector | Private |
| PI Contribution | Commercialisation of DNA modification antibodies we have made |
| Collaborator Contribution | Commercialisation of DNA modification antibodies we have made |
| Impact | Commercially available antibodies |
| Start Year | 2016 |
| Description | Analysis of Mll2-deficient oocytes |
| Organisation | Technical University of Dresden |
| Department | Biotechnology Center |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | We framed the biological question and were responsible for experimental design and data analysis. We performed DNA methylation and histone modification analysis of oocytes conditionally deleted for Mll2 provided by the partner. |
| Collaborator Contribution | Provided mice with conditional knock-out allele of Mll2 and Mll2-deficient oocytes. |
| Impact | Joint publication: Hanna et al., Nat. Struct. Mol. Biol. 2018. Deposited sequence datasets at NCBI GEO: GSE93941. |
| Start Year | 2013 |
| Description | Austin Smith |
| Organisation | University of Cambridge |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Epigenomics and multi-omics sequencing |
| Collaborator Contribution | Human and mouse pluripotent stem cells |
| Impact | Several collaborative publications. |
| Start Year | 2013 |
| Description | Bioinformatic analysis of oocyte ChIP-seq data |
| Organisation | Helmholtz Zentrum München |
| Department | Institute of Computational Biology |
| Country | Germany |
| Sector | Private |
| PI Contribution | We provided ChIP-seq datasets for various histone modifications in mouse wild-type and genetic knock-out oocytes to enable partner to test new informatics tools they had developed. |
| Collaborator Contribution | The partner provided new informatics methods, based on multivariate Hidden-Markov Models, to enable analysis of multiple ChIP-seq datasets, with focus on multivariate comparisons. |
| Impact | Joint publication: Hanna et al., Nat. Struct. Mol. Biol. 2018. Deposited sequence datasets at NCBI GEO: GSE93941. Hosted visiting bioinformatics PhD student from collaborating lab. Inter-disciplinarity: partner provides modelling and informatics expertise, our group provides biological datasets. |
| Start Year | 2016 |
| Description | Chromatin remodelling in oocytes |
| Organisation | Technical University of Dresden |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | Project concept, data generation, data analysis |
| Collaborator Contribution | sample generation, data analysis |
| Impact | Joint publication: PMID: 35137160 |
| Start Year | 2010 |
| Description | Consultancy Cambridge Epigenetix |
| Organisation | Cambridge Epigenetix |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | I am a consultant for the company |
| Collaborator Contribution | None at present |
| Impact | Oxidative bisulfite sequencing |
| Start Year | 2012 |
| Description | DNA methylation analysis in oocytes genetically deficient for Kdm1a (Lsd1) and Kdm1b (Aof1) |
| Organisation | University of Texas |
| Department | M. D. Anderson Cancer Center |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | We were responsible for framing the project and experimental design and performed genome-wide DNA methylation analysis in oocytes genetically deficient in the H3K4me2 demethylases KDM1A or KDM1B, provided by the collaborating group. |
| Collaborator Contribution | The partner provided oocytes from mice genetically deficient in Kdm1a or Kdm1b; provided RNA-seq datasets for these oocytes. |
| Impact | Joint publications Stewart et al., 2015 Genes & Dev; Gahurova et al. 2017 Epigenetics & Chromatin. Deposited sequence datasets at NCBI GEO: GSE73803 and GSE74549. |
| Start Year | 2011 |
| Description | DNA methylation analysis in oocytes genetically deficient in Hira |
| Organisation | Medical Research Council (MRC) |
| Department | MRC Clinical Sciences Centre (CSC) |
| Country | United Kingdom |
| Sector | Public |
| PI Contribution | We performed single-cell, genome-wide bisulphite sequencing of oocytes deficient in H3.3 chaperone HIRA, provided by the collaborating group; using our newly established single-cell BS-seq method (Smallwood et al., 2014 Nat. Meths). |
| Collaborator Contribution | The partner was responsible for framing the project and experimental design and provided mouse oocytes conditionally deleted in Hira. |
| Impact | Joint publication Nashun et al. 2015 Mol. Cell. Deposited sequence datasets at NCBI GSE66629. |
| Start Year | 2014 |
| Description | DNA methylation dynamics in single cells |
| Organisation | University of Cambridge |
| Department | Gurdon Institute |
| Country | United Kingdom |
| Sector | Charity/Non Profit |
| PI Contribution | Contributed project design and single cell sequencing data |
| Collaborator Contribution | Contributed project design and mathematical models |
| Impact | First paper is under submission |
| Start Year | 2014 |
| Description | Deciduoma |
| Organisation | National Institute of Environmental Health Sciences |
| Department | Reproductive and Developmental Biology Laboratory |
| Country | United States |
| Sector | Public |
| PI Contribution | Characterisation of reproductive defects in older females |
| Collaborator Contribution | Decidualisation model to assess the effect in the absence of an implanting embryo |
| Impact | Collaborative publication (PMID: 28874785) |
| Start Year | 2016 |
| Description | Donal O'Carrol |
| Organisation | University of Edinburgh |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Helped with methylome sequencing and computational work. |
| Collaborator Contribution | Conceived and conducted the study. |
| Impact | Publication Vasiliauskaite et al 2017. Developmental biologists, epigeneticists, computational biologists. |
| Start Year | 2016 |
| Description | Gastrulation team |
| Organisation | University of Cambridge |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Conceived of the project and lead partner. Contributing single cell multi-omics and embryology. |
| Collaborator Contribution | Helped to conceive of the project and several experimental and computational contributions. |
| Impact | First publication Mohammed et al 2017. Experimental embryology, single cell biology, computation, modelling, epigenetics. |
| Start Year | 2014 |
| Description | Guiseppe Lupo |
| Organisation | Sapienza University of Rome |
| Country | Italy |
| Sector | Academic/University |
| PI Contribution | We have profiled the epigenome and transcriptome of aged neural stem cells. We have also hosted GL in our laboratory over two summers and provided training in epigenomics techniques. |
| Collaborator Contribution | Our partners derived and characterised young and aged neural stem cells, provide expertise in neural biology, and have given training to us in neural stem cell cultures. |
| Impact | Manuscript in preparation. |
| Start Year | 2014 |
| Description | Human Cell Atlas |
| Organisation | Broad Institute |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Contributed to formulating the white paper of the Human Cell Atlas. |
| Collaborator Contribution | Conceived the project and brought the international consortium together. |
| Impact | The white paper was published Regev et al 2017. It involves single cell biologists and computational and modelling scientists. |
| Start Year | 2016 |
| Description | Jenny Nichols mouse and human embryology |
| Organisation | University of Cambridge |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Single cell multi-omics, mutants of epigenetic regulators. |
| Collaborator Contribution | Mouse and human embryos |
| Impact | Several collaborative publications and two joint grants. Multi-disciplinary between epigenetics, multi-omics, embryology, stem cell research. |
| Start Year | 2014 |
| Description | Jenny Nichols, Austin Smith |
| Organisation | University of Cambridge |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Contributed to conceiving studies. Epigenetic profiling and analysis. |
| Collaborator Contribution | Conceived and conducted studies. |
| Impact | Publications Kalkan et al 2017, Guo et al 2017. Involves embryologists, stem cell biologists, computational biologists, epigeneticists. |
| Start Year | 2016 |
| Description | Millipore DNA modification antibodies |
| Organisation | Merck |
| Department | MilliporeSigma |
| Country | United States |
| Sector | Private |
| PI Contribution | Commercialisation of DNA modification antibodies we have made |
| Collaborator Contribution | Commercialisation of DNA modification antibodies we have made |
| Impact | DNA modification antibodies |
| Start Year | 2011 |
| Description | XIMBIO DNA modification antibodies |
| Organisation | Cancer Research Technology (CRT) |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Commercialisation of DNA modification antibodies we have made |
| Collaborator Contribution | Commercialisation of DNA modification antibodies we have made |
| Impact | DNA modification antibodies |
| Start Year | 2016 |
| Description | uNK cells |
| Organisation | University of Cambridge |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Characterisaiton of reproductive problems in older females. |
| Collaborator Contribution | Analysis of immune repertoire of implantation sites depending on maternal age. |
| Impact | Collaborative publication (PMID: 28874785). |
| Start Year | 2016 |
| Description | Babraham Schools Day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Annual event in which local GCSE and A-level students are hosted in my group and many other groups at the Institute for a day of practical demonstrations. |
| Year(s) Of Engagement Activity | Pre-2006,2006,2007,2008,2009,2010,2011,2012,2013,2014,2015,2016 |
| Description | Cambridge Science Festival |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Exhibit about Babraham's research at Cambridge Science Festival |
| Year(s) Of Engagement Activity | 2015 |
| Description | Cambridge Science Festival: Molecular Explorers, Cambridge (UK) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Exhibit at Cambridge Science Festival, engaging with visitors at stall explaining the science and advances made in the area, answering questions and discussing outstanding research goals |
| Year(s) Of Engagement Activity | 2017 |
| Description | Cold Spring Harbor Laboratory meeting: Stem Cell Biology, New York (USA) (VPG, CS) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Participation in confenrence, poster presentation by both post-doctoral reserachers involved in this activity (VPG and CS) and engagement in discussions and broadening of scientific horizon |
| Year(s) Of Engagement Activity | 2017 |
| Description | EMBL Mammalian Genetics and Genomics Conference (MH) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Invited speaker at AMBL Conference Mammalian genetic and Genomic, Heidelberg, Germany |
| Year(s) Of Engagement Activity | 2017 |
| Description | Genetics Society and BSDB Spring Meeting, Warwick, UK (MH) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Invited speaker at annual spring meeting of the Genetics Society and British Society for Developmental Biology (BSDB) in Warwick, UK (2017) |
| Year(s) Of Engagement Activity | 2017 |
| Description | IFPA Meeting, Manchester, UK (MH) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Invited talk at IFPA Meeting held in Manchester, UK (2017) |
| Year(s) Of Engagement Activity | 2017 |
| Description | ISSHP Meeting, Berlin, Germany (MH) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Postgraduate students |
| Results and Impact | Invited talk at ISSHP Meeting held in Berlin, Germany (2017) |
| Year(s) Of Engagement Activity | 2017 |
| Description | Keynote speaker, CTR Anniversary Meeting, Cambridge, UK (MH) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Keynote lecture at 10th Anniversary Meeting of the Centre for Trophoblast Research, University of Cambridge (2017) |
| Year(s) Of Engagement Activity | 2017 |
| Description | Member, Board of Managers at Centre for Trophoblast Research |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | Yes |
| Geographic Reach | International |
| Primary Audience | Supporters |
| Results and Impact | As member of the Board of Managers at the CTR in Cambridge we make decisions on the award of PhD scholarships, postdoctoral fellowships and direct the general focal points of local research activity in the field of placental biology. PhD students admitted to the University of Cambridge; postdoctoral researchers' career path enhanced |
| Year(s) Of Engagement Activity | 2013,2014,2015,2016 |
| Description | Podcast with Dr Kat Arney on the impact of placentation and maternal age on reproductive outcome |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Podcast as descibed above |
| Year(s) Of Engagement Activity | 2017 |
| Description | Postgraduate course Epigenetics in Reproductive Biology, University of Murcia, Spain (VPG) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Postgraduate students |
| Results and Impact | Invited talk and workshop participation as part of postgraduate course on reproductive epigenetics |
| Year(s) Of Engagement Activity | 2016,2017 |
| Description | Press interview on Maternal methylation inheritance |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Co-interviewee which formed part of a televised clip reporting on a recent publication and its societal/public impact. |
| Year(s) Of Engagement Activity | 2016 |
| Description | Press release - Keeping eggs fresh with epigenetics |
| Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Media (as a channel to the public) |
| Results and Impact | Press release from Babraham to accompany publication of paper 'MLL2 conveys transcription-independent H3K4me3 in the oocyte', by Hanna et al. Nat. Struct. Mol. Biol. 2018. Picked up by 9 news outlets and blogs. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.babraham.ac.uk/news/2018/01/keeping-egg-cells-fresh-with-epigenetics |
| Description | Radio interview DeutschlandFunk |
| Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Radio Interview for DeutschlandFunk on the impact of maternal age on pregnancy success, irrespective of the increased risk of defects in the egg |
| Year(s) Of Engagement Activity | 2017 |
| URL | http://www.deutschlandfunk.de/alter-des-uterus-entscheidend-junges-glueck-in-jungen.676.de.html?dram... |
| Description | Royal Society Partnering Award - CS + LW, NM, SC |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Successful application for Royal Society Partnering Award. Poster 6 selected 6th form students over the course of 1 week and conducted in-depth research project. Data analysis followed at the pupils' school directly. |
| Year(s) Of Engagement Activity | 2016,2017 |
| Description | STEM teacher visit |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | As part of a week-long visit to the Institute, my group hosted 3 biology A-level teachers from local schools for involvement in practical work in the lab over a 2-day period. |
| Year(s) Of Engagement Activity | 2016 |
| Description | School's Day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | Yes |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | provided basic explanation of early development and the importance of the placenta for growth of a baby; hands-on experience for students in staining and microscopic analysis of a mouse placenta rose awareness of research activities and importance of basic research to understand common pregnancy-associated diseases |
| Year(s) Of Engagement Activity | 2006,2007,2008,2009,2010,2011,2012,2013,2014,2015,2016 |
| Description | Schools Day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Groups of 5-6 6th-form students take part in a one-day visit to 2 research groups at the Babraham Institute and conduct two small research experiments during that day. |
| Year(s) Of Engagement Activity | 2017,2018 |
| Description | Schools Day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Project design, scientific background induction and practical supervision for 6th form students as well as teachers during Annual Schools Day. |
| Year(s) Of Engagement Activity | 2015,2016,2017 |
| Description | The Ageing Cell Conference, Cambridge, UK (MH) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Invited talk at The Ageing Cell Conference held in Cambridge, UK (2017) |
| Year(s) Of Engagement Activity | 2017 |
| Description | Twilight teacher training event, Bioscience lite-CRISPR-Cas9 genome editing- technology, applications and implication, Cambridge (UK) (VPG) |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Talk and Q&A session with high school teachers to update and inform them on state-of-the-art genome editing tools |
| Year(s) Of Engagement Activity | 2017 |