606: Signalling networks underlying plant defence responses
Lead Research Organisation:
University of Warwick
Department Name: Warwick HRI
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
The overall aim of this research is to identify key signaling components activated during Botrytis cinerea infection and to determine how these components interact to mediate plant defence responses. We also want to investigate how this defence signalling is integrated with other signaling networks such as that regulating senescence, and how specificity in terms of end responses is achieved. We are using transcriptome profiling to highlight potential regulatory genes, which are assessed using transgenic knockout or overexpressing lines. The role of regulatory genes affecting the host susceptiblity to this pathogen are investigated further using a variety of functional genomics techniques.
Planned Impact
unavailable
Organisations
People |
ORCID iD |
| Katherine Denby (Principal Investigator) |
Publications
Hickman R
(2017)
Architecture and Dynamics of the Jasmonic Acid Gene Regulatory Network
in The Plant Cell
Kiddle SJ
(2010)
Temporal clustering by affinity propagation reveals transcriptional modules in Arabidopsis thaliana.
in Bioinformatics (Oxford, England)
Morrissey ER
(2011)
Inferring the time-invariant topology of a nonlinear sparse gene regulatory network using fully Bayesian spline autoregression.
in Biostatistics (Oxford, England)
Morrissey ER
(2010)
On reverse engineering of gene interaction networks using time course data with repeated measurements.
in Bioinformatics (Oxford, England)
Penfold CA
(2012)
Nonparametric Bayesian inference for perturbed and orthologous gene regulatory networks.
in Bioinformatics (Oxford, England)
Savage R
(2009)
R/BHC: fast Bayesian hierarchical clustering for microarray data
in BMC Bioinformatics
Stegle O
(2010)
A robust Bayesian two-sample test for detecting intervals of differential gene expression in microarray time series.
in Journal of computational biology : a journal of computational molecular cell biology
Windram O
(2012)
Arabidopsis defense against Botrytis cinerea: chronology and regulation deciphered by high-resolution temporal transcriptomic analysis.
in The Plant cell
Xu H
(2020)
A Molecular Signal Integration Network Underpinning Arabidopsis Seed Germination.
in Current biology : CB
| Description | The key outputs of this grant were time series gene expression profiles of plants after infection with a fungal pathogen or during natural senescence. We also developed computational tools to analyse this data to determine the key genes influencing disease resistance and senescence. These findings were crucial in obtaining further funding (the PRESTA systems biology) grant which extended this work to other pathogens and abiotic stresses drought and high light. Eventually work initiated during this award is being used to speed up breeding of beneficial traits in crops. |
| Exploitation Route | The times series data sets are publicly available for other people to analyse and look at expression profiles of any plant gene. The tools developed during this award (and the related PRESTA project) are freely available for others to use. We have identified candidate regulators of the plant defence response and senescence |
| Sectors | Agriculture Food and Drink |
| URL | http://www2.warwick.ac.uk/fac/sci/lifesci/research/presta/data/ |
| Description | BBRC GCRF IAA University of York |
| Amount | £7,465 (GBP) |
| Organisation | University of York |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 01/2017 |
| End | 03/2017 |
| Description | ISCF Agri-food Technology Catalyst Seeding Award |
| Amount | £17,000 (GBP) |
| Funding ID | BB/SCA/York/17 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 07/2017 |
| End | 02/2018 |
| Description | Global Plant Council/SEB Stress Resilience Forum |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Presented talk and took part in discussions at international stress resilience forum in Iguazu Falls, Brazil. Helped write a White Paper emanating from the workshop. |
| Year(s) Of Engagement Activity | 2015 |
| Description | Plant and Animal Genomes Conference |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Gave a talk at the Plant and Animal Genomes conference in a Systems Genomics workshop. |
| Year(s) Of Engagement Activity | 2016 |