Fellowship in IBDV research
Lead Research Organisation:
THE PIRBRIGHT INSTITUTE
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
To initiate a research project to study (1) the molecular pathogenesis and (2) the basis of genetic resistance in chickens to the endemic avian pathogen infectious bursal disease virus (IBDV). The project will enhance and expand the remit of the avian endemic viruses group within the avian viral diseases programme. IBD is a major immunosuppressive infectious disease of chickens caused by the highly contagious Birnavirus IBDV. The fellowship will require a person to develop a research program to carry out research into IBD to investigate the viral gene functions as well as molecular pathogenic mechanisms of the disease. Initial studies will involve the development of a reverse genetics system for IBDV and to use inbred lines of chickens to characterise virus genes involved in attenuation and to identify their role in genetic resistance. Analysis of viral and host gene expression in infected cells will be undertaken to examine molecular changes to gain insights into the disease. The research will involve, though not restricted to, the establishment of IBDV strains of varying virulence to examine pathogenic effects including immunosuppressive effects in disease models, investigation of the molecular mechanisms of induction of apoptosis by IBDV in cells of the Bursa of Fabricius, by analysing the viral and host gene expression, the development of quantitative PCR tools to measure viral replication in the tissues of birds infected with IBDV, to carry out experiments in different lines of chickens to evaluate differences in virus replication, genetic resistance and viral and host gene expression and to Examine the global proteome changes in IBDV infected cells using 2D/HPLC to identify the pathogenic pathways.
Planned Impact
unavailable
People |
ORCID iD |
| Andrew Broadbent (Principal Investigator) |
Publications
Asfor AS
(2022)
Modeling Infectious Bursal Disease Virus (IBDV) Antigenic Drift In Vitro.
in Viruses
Broadbent A
(2018)
An <em>Ex Vivo</em> Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
in Journal of Visualized Experiments
Broadbent A
(2018)
An <em>Ex Vivo</em> Chicken Primary Bursal-cell Culture Model to Study Infectious Bursal Disease Virus Pathogenesis
in Journal of Visualized Experiments
Campbell EA
(2020)
Discrete Virus Factories Form in the Cytoplasm of Cells Coinfected with Two Replication-Competent Tagged Reporter Birnaviruses That Subsequently Coalesce over Time.
in Journal of virology
Ciccone NA
(2017)
Early pathogenesis during infectious bursal disease in susceptible chickens is associated with changes in B cell genomic methylation and loss of genome integrity.
in Developmental and comparative immunology
Dulwich K
(2017)
Differential gene expression in chicken primary B cells infected ex vivo with attenuated and very virulent strains of infectious bursal disease virus (IBDV)
in Journal of General Virology
| Description | 1.) Defining how IBDV replicates inside host cells: We have made the first ever tagged reporter birnaviruses and used them to determine that birnaviruses such as IBDV form virus factories (VFs) in the cytoplasm that look different to the VFs of other double stranded RNA viruses. We have shown that the VFs move in the cytoplasm, show liquid-like properties by fluorescence recovery after photobleaching (FRAP) and coalesce together over time. Moreover, we have demonstrated that during a co-infection, discrete VFs form from each input virus, which subsequently coalesce. We hypothesise that VF coalescence is required for reassortment to occur and current work is aimed at testing this hypothesis and using the tagged viruses to study superinfection exclusion. Using Correlative Light and Electron Microscopy, we have determined that the VFs are discrete regions of proteinaceous accumulations but are distinct from the paracrytalline arrays of virions observed by transmission electron microcopy. 2.) Determining how host- cells respond to viral infection: we have produced the first ever list of differentially regulated host-genes that are up and down-regulated upon IBDV infection of primary chicken bursal cells. 3.) Evaluating how viruses antagonise the host antiviral response: We have discovered that a very virulent (vv) strain of IBDV induces less antiviral Type I IFN responses than an attenuated cell-culture adapted vaccine strain of IBDV and a classical field strain of IBDV, consistent with the vv virus being able to inhibit antiviral responses to a greater extent than other strains. We find the same to be true in vitro and in vivo. Moreover, we have discovered that the viral VP4 gene is key in determining this differential type I IFN response: The VP4 protein from the vv strain inhibited the induction of Type I IFN, but the VP4 protein from a classical strain did not. We hypothesize that vv strains of virus are more virulent than other strains as they have evolved a VP4 protein capable of suppressing antiviral gene expression more effectively. 4.) Enhancing IBDV control: The VP4 protein may be a virulence factor. It may therefore be possible to design a rationally attenuated live vaccine virus by inserting the VP4 from an attenuated strain into a field strain to attenuate it, or by inserting the VP4 gene from a field strain into an over-attenuated vaccine to make it "hotter". We inoculated birds with an attenuated vaccine strain that caused no clinical signs, a vv virus that caused severe disease, and a chimeric vaccine strain containing the VP4 from a vv strain that caused mild disease, suggesting that VP4 contributes to virulence, but that virulence is a multi-factoral phenotype. 5.) Establishing the molecular basis of disease resistance. We infected 4 inbred lines of chickens, one described as "susceptible" and 3 described as "resistant" to disease and discovered that all lines were susceptible to infection but the line most resistant to disease was line 15. We conducted RNASeq on bursal tissue and determined that those more susceptible to severe disease (line W) had pathways associated with inflammation unregulated compared to the more resistant line 15. Moreover, inbred line W had a higher base-line number of macrophages that may be responsible for this phenomenon. We suggest that efforts to engineer disease resistant chickens should focus inflammatory pathways. |
| Exploitation Route | Current and future work is aimed at: 1.) understanding the nature of the Virus Factories, including their composition and ultrastructure, 2.) determining the molecular basis for the differential effects on type I IFN induction by different VP4 proteins. The tagged reporter viruses will also be used in super-infection exclusion experiments and in vivo, using transgenic GFP1-10 expressing birds from the Roslin Institute. Moreover, they will be a useful tool to other researchers working on IBDV and related viruses. |
| Sectors | Agriculture Food and Drink |
| Description | Antigenic characterisation of infectious bursal disease virus to improve vaccination strategies and vaccine design |
| Amount | £450,685 (GBP) |
| Funding ID | BB/S014594/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 01/2020 |
| End | 12/2023 |
| Description | Defining the circadian clock in chicken cells and its impact upon viral replication |
| Amount | £4,500 (GBP) |
| Organisation | The Houghton Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 11/2019 |
| End | 11/2020 |
| Description | Internal Seed Fund- Development of an IBDV diagnostic test that can differentiate infected from vaccinated animals, for use in surveillance. |
| Amount | £10,000 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 12/2019 |
| End | 02/2020 |
| Description | NC3Rs Research Project Grant |
| Amount | £436,339 (GBP) |
| Funding ID | NC/R001138/1 |
| Organisation | National Centre for the Replacement, Refinement and Reduction of Animals in Research (NC3Rs) |
| Sector | Public |
| Country | United Kingdom |
| Start | 12/2017 |
| End | 11/2019 |
| Description | PhD studentship |
| Amount | £102,800 (GBP) |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 09/2015 |
| End | 09/2019 |
| Description | Seed Catalyst Award |
| Amount | £25,527 (GBP) |
| Funding ID | ISCF-TFPSA-Pirbright |
| Organisation | The Pirbright Institute |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 11/2018 |
| End | 02/2019 |
| Description | Small Research Project Grant |
| Amount | £8,000 (GBP) |
| Organisation | The Houghton Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 09/2015 |
| End | 09/2016 |
| Description | Towards a novel multivalent poultry vaccine: Development of a recombinant infectious bursal disease vectored vaccine encoding conserved B- and T- cell epitopes from infectious bronchitis virus |
| Amount | £5,000 (GBP) |
| Organisation | The Houghton Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 11/2019 |
| End | 11/2020 |
| Description | [YY-EEID US-UK XXXX] Evaluating how immunosuppression influences influenza A virus transmission and evolution in wild and domestic birds |
| Amount | £606,360 (GBP) |
| Funding ID | BB/T008806/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 06/2019 |
| End | 06/2023 |
| Title | An ex vivo IBDV infection model using a chicken primary B cell culture system |
| Description | Recently, it has become possible to culture chicken primary B cells in vitro in the presence of a soluble construct of chicken CD40L that was made at The Pirbright Institute. We have demonstrated that these chicken primary B cells can be infected with infectious bursal disease virus (IBDV). This system will be validated and expanded to other B cell tropic viruses in order to replace the use of infected chickens in research, in an NC3Rs funded grant. |
| Type Of Material | Model of mechanisms or symptoms - in vitro |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | Using this system, we have determined that chicken primary B cells respond to IBDV infection by inducing Type I IFN responses. However, the induction is more pronounced in cells infected with an attenuated strain compared to a very virulent strain. This is consistent with the very virulent strain down-regulating antiviral responses to a greater extent than other strains which may, in part, explain its enhanced virulence. We also observed a reduction in the expression of key genes involved in B cell proliferation and activation following IBDV infection which was only possible by using the primary B cell culture system. |
| URL | https://www.ncbi.nlm.nih.gov/pubmed/?term=broadbent+dulwich |
| Title | Correlative Light and Electron Microscopy (CLEM) on IBDV-infected cells |
| Description | Jenny Simpson and Pippa Hawes have developed the technique of Correlative Light and Electron Microscopy (CLEM). This technique has been used to visualise Virus Factories of IBDV. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2019 |
| Provided To Others? | No |
| Impact | The Virus Factories (VFs) of IBDV were thought to be paracrystalline arrays of virions in the cytoplasm. However, combining our GFP11-tagged IBDV with CLEM technology revealed that the VFs are actually proteinaceous accumulations in the cytoplasm. This has opened up a new avenue of research in terms of the ultrastructure, formation and function of the VFs. |
| Title | Embryonic chicken primary bursal cell culture system |
| Description | We expanded our primary bursal cell culture system to include embryonic bursal cells. |
| Type Of Material | Model of mechanisms or symptoms - in vitro |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | This opens up the possibility of labs using the primary bursal cells even in settings where animal facilities are not available. |
| Title | Fluorescence Recovery after Photobleaching (FRAP) of infectious bursal disease virus (IBDV) replication factories |
| Description | Fluorescence Recovery after Photobleaching (FRAP) of infectious bursal disease virus (IBDV) replication factories. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2020 |
| Provided To Others? | No |
| Impact | We have used this technology to determine that IBDV replication factories display qualities of liquid-liquid phase separation |
| Title | IBDV Reverse Genetics System |
| Description | We have developed a reverse genetics system for IBDV. |
| Type Of Material | Technology assay or reagent |
| Provided To Others? | No |
| Impact | We have used the reverse genetics system to generate recombinant IBDVs and chimeric recombinant IBDVs that contain genes from classical and very virulent strains of IBDV in the background of a cell culture adapted attenuated strain. This will enable us to study the effect of individual virus genes on pathogenicity. |
| Title | IBDV titration in chicken B cells by TCID50 to replace IBDV titration in chicken embryos by EID50 |
| Description | The titer of IBDV has traditionally been determined by infecting embryos, humanely culling them at embryonic day 18 and then observing the number that show growth abnormalities due to virus. As this goes beyond 2/3 gestation (14 embryonic days), this falls under Home Office Legislation. We demonstrated that the immortalised B cell line, DT40, could be used to titrate IBDV instead, replacing the use of embryos. This is being written up into a manuscript currently. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2019 |
| Provided To Others? | No |
| Impact | we have already replaced the use of embryos in our research. Once this has been published and is in the public domain, we anticipate this will lead to more replacement in the field, |
| Title | Stable chicken cell lines overexpressing chicken IFITM1,2 and 3 |
| Description | In collaboration with Mark Fife, we have produced chicken stable cell lines overexpressing chicken IFITM1,2, 3 and a mutant chicken IFITM3 lacking palmitoylation sites. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | These cell lines will be used to evaluate the effect of the IFITM proteins on the replication of different viruses, including IBDV. |
| Title | chimeric IBDVs |
| Description | Andrew Broadbent has engineered chimeric IBDVs expressing individual genes from a very virulent strain in the backbone of an attenuated strain. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | These chimeric strains can be used to define viral determinants of virulence. |
| Title | in vitro model of IBDV antigenic drift in chicken B cells |
| Description | IBDV vaccines do not induce sterilising immunity, meaning vaccinated birds can become infected with field strains. As for other viruses, it is thought that vaccine-induced immune selection pressure drives the evolution of antigenic drift variants that accumulate amino acid changes in the hypervariable region of the capsid, which may lead to vaccine failures. However, antigenic drift has not been studied in any detail for IBDV, and there is therefore a paucity of information regarding how plastic the capsid is, how quickly mutations arise and become fixed in the virus population, or whether some are more dominant than others. One way of studying this is to vaccinate chickens with sub-protective doses and then challenge them with field strains of IBDV and then sequence the resulting viruses that emerge. To replace the use of birds in these experiments, we have developed an in vitro model of antigenic drift by serially passaging a field strain, F5270, in the immortalised chicken B cell line, DT40, in the presence of sub-neutralising concentrations of vaccine-induced antibodies to better characterise changes in the capsid. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2019 |
| Provided To Others? | No |
| Impact | In our model, amino acid mutations arose in the hypervariable region of the IBDV capsid at the same sites previously observed in field studies, validating our model. Furthermore, we demonstrated that mutations arose early, by only 5 passages in chicken B cells, which subsequently became fixed by 10 passages. We are now doing next generation sequencing to better characterise the plasticity of the capsid. |
| Title | in vitro quantification of anti-IBDV neutralising antibodies using chicken B cells |
| Description | Typically, the titre of neutralising antibodies generated by a field strain of IBDV is quantified by determining the ability of the serum to neutralise the infectivity of a cell- culture adapted strain of IBDV into immortalised fibroblast cells. The reason for this is that field strains do not typically infect immortalised fibroblast cells and have a tropism limited to B lymphocytes. However, this technique may not quantify the correct titre against the field strain in question as it relies on cross-reactivity of the serum to the cell-culture adapted strain. We have developed an in vitro neutralisation assay using the immortalised B cell line, DT40, in order to quantify the titre of neutralising antibodies against field strains of IBDV. As cytopathic effect is not observed in lymphocytes, this assay relies on immunostaining with antibodies against IBDV to determine the endpoint. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2019 |
| Provided To Others? | No |
| Impact | This technique enables us to more accurately quantify the titre of antibodies against field strains of IBDV. |
| Title | replication competent recombinant split-GFP infectious bursal disease virus |
| Description | We have generated a replication competent recombinant split-GFP infectious bursal disease virus (IBDV) where a small GFP11 molecule is tagged to the virus polymerase (VP1) to make IBDV-VP1-GFP11. Infected cells that express the GFP1-10 molecule fluoresce green when the GFP11 tag complements the GFP1-10. The IBDV-VP1-GFP11 colocalises with VP3 and dsRNA and therefore is likely to be present at the sites of virus replication. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2017 |
| Provided To Others? | No |
| Impact | This tool can be used to study IBDV replication complex development in infected cells. |
| Title | tetracysteine-tagged IBDV |
| Description | Andrew Broadbent has engineered a tetracysteine (TC)-tagged IBDV that can be used in live cell imaging and co-infection studies with the split-GFP IBDV. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | This tool, combined with the split-GFP IBDV, will enable us to study co-infection, super-infection exclusion, and the intracellular requirements for viral reassortment. |
| Title | E-MTAB-5947- Transcription profiling by array of differential gene expression in chicken primary B cells infected ex vivo with attenuated and very virulent strains of infectious bursal disease virus (IBDV) |
| Description | Microarray data submitted to ArrayExpress from chicken primary B cells infected with a very virulent and attenuated strain of IBDV. |
| Type Of Material | Database/Collection of data |
| Year Produced | 2017 |
| Provided To Others? | Yes |
| Impact | This is the first ever microarray data of chicken primary B cells infected with IBDV and will be of use to other researchers in the area of B cell biology or IBDV virology |
| URL | https://www.ebi.ac.uk/arrayexpress/experiments/E-MTAB-5947/ |
| Description | Dr Alex Schock, APHA - Detemining the prevalence and sequences of IBDV in vaccinated chicken flocks in the UK |
| Organisation | Animal and Plant Health Agency |
| Country | United Kingdom |
| Sector | Public |
| PI Contribution | We will determine whether samples are positive for IBDV. If they are positive, we will amplify the hypervariable region of the capsid by PCR and send for Sanger Sequencing |
| Collaborator Contribution | APHA will obtain samples from Veterinary Practices and perform histology. |
| Impact | we have been successful in obtaining funding (Pirbright Internal Seed Award, £10,000) |
| Start Year | 2019 |
| Description | Dr Carol Cardona, University of Minnesota- avian influenza virus challenge of IBDV or mock-infected chickens |
| Organisation | University of Minnesota |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | We will challenge birds that have been exposed to IBDV, or mock exposed, with highly pathogenic avian influenza (HPAI) strains to determine how IBDV-mediated immunosuppression impacts upon HPAI infection in chickens. |
| Collaborator Contribution | Dr Cardona's group will challenge birds that have been exposed to IBDV, or mock exposed, with low pathogenic avian influenza (LPAI) strains to determine how IBDV-mediated immunosuppression impacts upon LPAI infection in chickens. |
| Impact | we have been successful in obtaining funding (Grant Ref: BB/T008806/1) |
| Start Year | 2019 |
| Description | Dr David Welchman, APHA -Detemining the prevalence and sequences of IBDV in vaccinated chicken flocks in the UK |
| Organisation | Animal and Plant Health Agency |
| Country | United Kingdom |
| Sector | Public |
| PI Contribution | We wiil determine whether samples are positive for IBDV. If they are positive, we will amplify the hypervariable region of the capsid by PCR and send for Sanger Sequencing |
| Collaborator Contribution | APHA will obtain samples from Veterinary Practices and perform histology. |
| Impact | we have been successful in obtaining funding (Pirbright Internal Seed Award, £10,000) |
| Start Year | 2019 |
| Description | Dr Holly Shelton, The Pirbright Institute- avian influenza virus challenge of IBDV or mock-infected chickens |
| Organisation | The Pirbright Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We will expose birds to IBDV, compared to mock controls |
| Collaborator Contribution | Holly will train my group in conducting studies with avian influenza viruses and, together, we will challenge birds with highly pathogenic avian influenza (HPAI) strains to determine how IBDV-mediated immunosuppression impacts upon HPAI infection in chickens. |
| Impact | we have been successful in obtaining funding (Grant Ref: BB/T008806/1) |
| Start Year | 2019 |
| Description | Dr Mark Fife, The Pirbright Institute |
| Organisation | The Pirbright Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We aim to determine if chicken IFITM proteins restrict the replication of the IBDV. We have access to different strains of the virus and we have the expertise necessary to quantify viral replication in cell-lines by different means. |
| Collaborator Contribution | Dr Mark Fife's team have the specialist reagents, for example expression plasmids, antibodies, CRISPR tools etc to produce either chicken cell-lines that stably overexpress chicken IFITM proteins, or that have the chicken IFITM proteins knocked out. These cell-lines are required to complete the project. |
| Impact | Drs Andrew Broadbent and Mark Fife co-supervise an undergraduate industrial placement student who is working on this project. |
| Start Year | 2015 |
| Description | Dr Michael Skinner, Imperial College London |
| Organisation | Imperial College London |
| Department | Department of Primary Care and Public Health |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We aim to compare the host transcriptional response of primary chicken B cells infected with different strains of IBDV of varying virulence. We have the specialist reagents and expertise in house that are required to culture primary chicken B cells, and we have access to different strains of IBDV. |
| Collaborator Contribution | Dr Michael Skinner and his team at Imperial College London have the expertise and facilities to generate and analyse RNA-Seq data in order to determine host transcriptional responses. This is required in order to complete the aim of the project. |
| Impact | Further funding: PhD studentship (£102,800) - Dr Andrew Broadbent at The Pirbright Institute and Dr Michael Skinner at Imperial College London are joint supervisors for a PhD student working on this project. Further Funding: Houghton Trust (£8,000)- Dr Andrew Broadbent was awarded a Houghton Trust Small Research Grant to contribute to this project. Further funding: NC3Rs (Grant Ref: NC/R001138/1) |
| Start Year | 2014 |
| Description | Dr Rachel Edgar, Imperial College London- defining the circadian clock in chicken cells and how this impacts upon viral replication |
| Organisation | Imperial College London |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | we will supply sequences of chicken clock genes and chicken cells and challenge synchronised chicken cells with IBDV and perform in vivo experiments |
| Collaborator Contribution | Dr Edagr's group will synchronise chicken cells and characterise the oscillatory expression of chicken clock genes. |
| Impact | we have been successful in obtaining funding (Houghton Trust, £4,500) |
| Start Year | 2019 |
| Description | Professor Helen Sang,The Roslin Institute - GFP1-10 expressing transgenic chickens |
| Organisation | University of Edinburgh |
| Department | The Roslin Institute |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | We have developed IBDV tagged to GFP11 |
| Collaborator Contribution | Helen Sang's group at the Roslin Institute have developed transcgenic chicken lines that constitutively express GFP1-10 in every cell. |
| Impact | we have been successful in obtaining funding (Grant Ref: BB/S014594/1) |
| Start Year | 2019 |
| Description | Professor Oliver Pybus, University of Oxford / Royal Veterinary College - IBDV-like viruses in wild bird populations |
| Organisation | University of Oxford |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | My group will characterise the immunosuppressive potential of IBDV-like viruses from wild bird populations |
| Collaborator Contribution | Oliver Pybus' group have screened a wild bird population by metagenomics shotgun sequencing to determine the prevalence of IBDV-like viruses and correlate their presence with the abundance and diversity of viral sequences identified for other viruses. |
| Impact | we have been successful in obtaining funding (Grant Ref: BB/T008806/1) |
| Start Year | 2019 |
| Description | A talk at a symposium entitled: Using the 3Rs to support good science |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | I gave a talk at a Symposium held at The Pirbright Institute entitled: "Using the 3Rs to support good science". The talk was entitled: "A chicken primary B cell culture model to study the pathogenesis and improve the control of immunosuppressive viruses of poultry" |
| Year(s) Of Engagement Activity | 2017 |
| Description | A talk at the British Poultry Diseases Group meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | The Poultry Diseases Group meet quarterly and is comprised of representatives from private veterinary practices, vaccine and pharmaceutical industries, and the Animal and Plant Health Agency (APHA). I gave a talk at one of the meetings outlining my research to date and future directions. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Cheltenham Science Festival 2019 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | Kate Dulwich participated in an outreach activity at the Cheltenham Science Festival from 07-09 Jun 2019 |
| Year(s) Of Engagement Activity | 2019 |
| Description | Elle Campbell Presented her data at the 2019 Microbiology Society Annual Meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Elle Campbell gave an oral presentation at the 2019 Microbiology Society Annual Meeting on the work we were doing with fluorescently tagged IBDVs and virus factory movement. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Innovate Guilford |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | Dr Broadbent gave a talk at 'Innovate Guilford' Festival outlining the work we are doing with Avian Virus Vaccines, including those against IBDV. The aim was to raise public awareness on different avian viruses, and genetically modified vaccines. |
| Year(s) Of Engagement Activity | 2017 |
| Description | Invited speaker at the British VeterinaryPoultry Association 2018 meeting at Boehringer Ingelheim |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Industry/Business |
| Results and Impact | I was invited to present my research findings at the British Veterinary Poultry Association 2018 meeting, held at Boehringer Ingelheim. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Invited speaker at the Large Animal Research Network (LARN) 2018 meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | I presented the aims and objectives of the NC3Rs project to the Large Animal Research Network (LARN). |
| Year(s) Of Engagement Activity | 2018 |
| Description | Poster Presentation at the 2020 British Society for Immunology Virtual meeting: "Connecting Immunology in the time of COVID-19" |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | Andrew Broadbent presented a digital poster at the British Society for Immunology 2020 online meeting entitled "Connecting Immunology in the time of COVID-19". |
| Year(s) Of Engagement Activity | 2020 |
| URL | https://www.immunology.org/events/bsi-congress |
| Description | Represented The Pirbright Institute at the British Pig and Poultry Fair, 2018 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Dr Andrew Broadbent represented The Pirbright Institute at the British Pig and Poultry Fair, 2019 that was well attended by members of the General Public, poultry industry representatives and veterinary practitioners. |
| Year(s) Of Engagement Activity | 2018 |
| URL | https://www.pigandpoultry.org.uk/ |
| Description | Teaching- lecturing, small group classroom teaching and laboratory practical teaching |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Postgraduate students |
| Results and Impact | I have been actively engaged with teaching activities at the University of Surrey, giving lectures, small group classes and laboratory practical classes to vet students and MSc students. |
| Year(s) Of Engagement Activity | 2015,2016,2017 |
| Description | University of Surrey Open Day |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | I represented The Pirbright Institute at the University of Surrey Vet School open day, talking about my research and what it is like to be a research scientist. |
| Year(s) Of Engagement Activity | 2015 |
| Description | University of Surrey School of Veterinary Medicine Open Day- Dec 2019 |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | engaged with parents and 6th form students about a career as a Veterinarian and specifically a career in Veterinary Research |
| Year(s) Of Engagement Activity | 2019 |
| Description | University of Surrey School of Veterinary Medicine Open Day- Jul 2019 |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Public/other audiences |
| Results and Impact | engaged with parents and 6th form students about a career as a Veterinarian and specifically a career in Veterinary Research |
| Year(s) Of Engagement Activity | 2019 |
| Description | Volunteered at a school open day |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | I represented The Pirbright Institute talking at a school about my research and what it is like to be a research scientist. |
| Year(s) Of Engagement Activity | 2015 |
| Description | a talk at the Recently Independent Virology Investigators meeting |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Professional Practitioners |
| Results and Impact | I gave a talks outlining my current research and future plans. |
| Year(s) Of Engagement Activity | 2015,2016,2017 |
| Description | talk to the U3A |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | I gave a talk to the University of the 3rd Age (U3A)- a group of the general public above the age of 65. |
| Year(s) Of Engagement Activity | 2016 |