Chemical glycobiology
Lead Research Organisation:
John Innes Centre
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
The Field group is diverse in its activities, which range from organic synthesis to mechanistic enzymology and chemical biology. The common theme that runs throughout is carbohydrates. Current research themes include: the development of novel chemical and enzymatic approaches for the preparation of sugar nucleotides, oligosaccharides and glycosylated natural products; structural and mechanistic studies on enzymes involved in bacterial lipopolysaccharide, plant cell wall, plant and microbial and secondary metabolite biosynthesis; carbohydrate-coated surfaces (carbohydrate microarrays, glyco-nanoparticles and glyco-quantum dots) for identifying and quantifying protein-carbohydrate and pathogen-carbohydrate interactions; chemical genomics and chemical proteomics tools for plant cell and developmental biology with a particular emphasis on glycobiology (starch and plant cell metabolism, glycosylated secondary metabolites).
Planned Impact
unavailable
People |
ORCID iD |
| Rob Field (Principal Investigator) |
Publications
Ahmadipour S
(2019)
Chemoenzymatic Synthesis of C6-Modified Sugar Nucleotides To Probe the GDP-d-Mannose Dehydrogenase from Pseudomonas aeruginosa.
in Organic letters
Aldholmi M
(2022)
Euglenatides, Potent Antiproliferative Cyclic Peptides Isolated from the Freshwater Photosynthetic Microalga Euglena gracilis.
in Angewandte Chemie (International ed. in English)
Alphey MS
(2013)
Allosteric competitive inhibitors of the glucose-1-phosphate thymidylyltransferase (RmlA) from Pseudomonas aeruginosa.
in ACS chemical biology
Andriotis V
(2016)
Cell wall degradation is required for normal starch mobilisation in barley endosperm
in Scientific Reports
Andriotis VM
(2016)
The Maltase Involved in Starch Metabolism in Barley Endosperm Is Encoded by a Single Gene.
in PloS one
Andriotis VM
(2016)
Iminosugar inhibitors of carbohydrate-active enzymes that underpin cereal grain germination and endosperm metabolism.
in Biochemical Society transactions
Aragão-Leoneti V
(2010)
Application of copper(I)-catalysed azide/alkyne cycloaddition (CuAAC) 'click chemistry' in carbohydrate drug and neoglycopolymer synthesis
in Tetrahedron
Ballell L
(2007)
New Small-Molecule Synthetic Antimycobacterials
in Antimicrobial Agents and Chemotherapy
Ballell L
(2007)
New thiopyrazolo[3,4-d]pyrimidine derivatives as anti-mycobacterial agents.
in Bioorganic & medicinal chemistry letters
Baltina L
(2010)
Synthesis and anti-HIV activity of triterpene 3-O-galactopyranosides, analogs of glycyrrhizic acid
in Chemistry of Natural Compounds
| Description | New information on inhibitors of enzymes in starch metabolism, in model plant Arabidopsis and in barley. A new carbohydrate-binding virus that infect and lyse algae - a key factor in releasing fish-killing toxins from algal blooms. New enzymatic approaches for the preparation of oligosaccharides, based on glycoside phosphorylases and glycan sucrases Protein X-ray crystallography studies on enzymes involved in starch metabolism New carbohydrate-coated surfaces (glyco-nanoparticles) for identifying pathogen-carbohydrate interactions |
| Exploitation Route | Our virus/algal blooms work impacts on policy for waterways management and mitigation strategies. This is being followed up with the Environment Agency and the Broads Authority. Our enzymatic synthesis work has been taken forward with Innovate UK support - via the Glycoenzymes and GelEnz consortia. |
| Sectors | Agriculture Food and Drink Chemicals Environment Healthcare Manufacturing including Industrial Biotechology Pharmaceuticals and Medical Biotechnology |
| Description | Our work on glycosylated natural product toxins has had a major impact on algal bloom management on the Norfolk Broads, from work with the Environment Agency and the Broads Authority. Our enzymatic synthesis work has been taken forward with Innovate UK support - via the Glycoenzymes and GelEnz consortia. It has also been central to training ECRs from Thailand, with four returning to their homeland to take up academic positions (Tantanarat, Kuaudomlarp, Panpetch, Charoenwongpaiboon), all of whom continue to work in carbohydrate biotechnology. |
| First Year Of Impact | 2019 |
| Sector | Agriculture, Food and Drink,Environment,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Societal Economic Policy & public services |
| Title | Enzymatic synthesis of HMO analogies |
| Description | Through detailed kinetic analysis, we have been able to understand how to use cellodextrin phosphorylase to transfer non-natural substrates to generate new to nature human milk oligosaccharide analogues |
| Type Of Material | Biological samples |
| Year Produced | 2020 |
| Provided To Others? | Yes |
| Impact | Further studies ongoing to explore HMO analogue bioactivity. |
| Description | CDs |
| Organisation | Newcastle University |
| Country | United Kingdom |
| PI Contribution | Evaluation of a glycosynthase for novel cyclodextrin production |
| Collaborator Contribution | Provision of clone of wild type enzyme and active site mutant |
| Impact | Too early to judge |
| Start Year | 2017 |
| Description | Glycan synthesis |
| Organisation | Iceni Diagnostics |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | Exchange of ideas and staff with regards the chemical synthesis of glycans and sugar phosphates. |
| Collaborator Contribution | Exchange of ideas and staff |
| Impact | Improved methods for the chemical synthesis of glycans and sugar phosphates. |
| Start Year | 2017 |
| Description | Glycoarrays |
| Organisation | Newcastle University |
| Country | United Kingdom |
| PI Contribution | Collaboration on glycoprofiling using microarray approaches to define the carbohydrate content of commercial prebiotics from Bangladesh. |
| Collaborator Contribution | Access to facilities and expertise to print carbohydrate microarrays. |
| Impact | Information for inclusion in publication in due course. |
| Start Year | 2017 |
| Description | MD starch |
| Organisation | University of Manchester |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Experimental evaluation of glycan structure and enzyme sensitivityt |
| Collaborator Contribution | Microsecond molecular dynamics simulation to assess glycan-glycan interactions |
| Impact | Too early |
| Start Year | 2017 |
| Description | PPase |
| Organisation | University of York |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Work up of scalable synthesis of N-glycan analogues |
| Collaborator Contribution | Clone of N-glycan phosphorylase |
| Impact | Too early |
| Start Year | 2017 |
| Company Name | Iceni Glycoscience |
| Description | Iceni Glycoscience develops carbohydrate-based therapeutics and point-of-care diagnostics for infectious diseases using their own research in glycoscience, nanoparticle technology, and bioconjugation chemistry. |
| Year Established | 2014 |
| Impact | Too early |
| Website | http://www.icenidiagnostics.com |
| Description | Algae on the Norfolk Broads |
| Form Of Engagement Activity | A formal working group, expert panel or dialogue |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Third sector organisations |
| Results and Impact | A series of stakeholder engagement and Pint of Science events were held to communicate to the public and relevant GROs and NGOs |
| Year(s) Of Engagement Activity | 2015,2016,2017,2018,2020 |