Development of real-time biosensors
Lead Research Organisation:
John Innes Centre
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
Bacteria are able to detect a large array of natural products that they can transport, degrade or modify. For example plant associated bacteria such as Rhizobium and Pseudomonas synthesise hundreds of specific ABC transport systems that are induced by a diverse array of sugars, amino acids, flavonoids and alkaloids. We are developing ABC solute binding proteins to produce FRET based biosensors that report the presence of their natural ligands. Metagenomic biosensor libraries of sequenced bacteria are also being screened for the detection of novel metabolic pathways involved in the modification and break down of natural products.
Planned Impact
unavailable
Organisations
People |
ORCID iD |
| Philip Poole (Principal Investigator) |
Publications
Bourdès A
(2012)
Mining the Sinorhizobium meliloti transportome to develop FRET biosensors for sugars, dicarboxylates and cyclic polyols.
in PloS one
East AK
(2008)
Biosensors for ligand detection.
in Advances in applied microbiology
Poole P
(2017)
Shining a light on the dark world of plant root-microbe interactions.
in Proceedings of the National Academy of Sciences of the United States of America
Ramachandran VK
(2011)
Adaptation of Rhizobium leguminosarum to pea, alfalfa and sugar beet rhizospheres investigated by comparative transcriptomics.
in Genome biology
| Description | Botanic Gardens seminar |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | I talked to the Botanic Gardens about nitrogen and humanity Lots of questions and feedback. This is widely advertised to the public |
| Year(s) Of Engagement Activity | 2014 |
| Description | Give peas a chance |
| Form Of Engagement Activity | A magazine, newsletter or online publication |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | We made a film with Oxford sparks on nitrogen fixation Lots os hits on the web site |
| Year(s) Of Engagement Activity | 2014 |
| Description | Meeting minds Oxford Alumni |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | I gave a talk to the Oxford biological sciences Alumni Enthusiastic response from the audience |
| Year(s) Of Engagement Activity | 2014 |
| Description | Schools one week Intensive course |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Schools |
| Results and Impact | Intensive one week course for A level students in practical plant and microbial science Students all keen to apply for science based university courses |
| Year(s) Of Engagement Activity | 2014 |
| Description | Wymondham academy school visit |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Schools |
| Results and Impact | Talk to 6th form students to encourage them to consider science as a career Students keen to apply for university places |
| Year(s) Of Engagement Activity | 2014 |