Prediction of F1 hybrid performance in Winter Oilseed Rape
Lead Research Organisation:
John Innes Centre
Department Name: UNLISTED
Abstract
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Technical Summary
We aim to develop a methodology to identify sequence-based markers that are predictive of crop performance and that can be used to make crop breeding faster and more efficient. As an exemplar, we have chosen hybrid oilseed rape. Hybrid plants (i.e. those derived by crossing two inbred parent lines) often outperform their parents, a phenomenon known as hybrid viogour or heterosis. This provides opportunities for improvement of productivity and environmental sustainability. To permit efficient breeding and realize this potential, molecular markers predictive of hybrid performance are required. Conventional approaches have been unsuccessful as the density of markers available has been far too low to find such associations. To overcome this, high throughput sequencing will be used to simultaneously identify variation in gene sequences and quantify gene expression in the parents of a panel of ~150 hybrids for which performance is known or will be determined in the initial phase of the project. Using a combination of 3 approaches, correlations between sequence-based variation and performance for a range of traits will be identified. Hybrids with new combinations of markers predicted to give enhanced performance will be developed and the performance validated by on-farm trialling.
Planned Impact
unavailable
People |
ORCID iD |
| Ian Bancroft (Principal Investigator) |
| Description | The extent or radish genomic introgression containing the fertility restorer component of the Ogura hybrid system was elucidated and markers relating variants in this with crop performance identified. |
| Exploitation Route | The findings will be taken forward by the rapeseed breeding industry. This will be done by selecting plants to develop as parents of hybrid cultivars by using marker-assisted selection for combining ability during breeding. This will accelerate the breeding process for developing new hybrid cultivars. This is important to enable new traits, such as disease resistance, to be rapidly incorporated into high-yielding elite cultivars. If successful cultivars can be developed, the downstream industry who will benefit initially are farmers and seed crushers. End users would include both users of industrial rapeseed oil (e.g. biodiesel) and the public via healthy vegetable oil for cooking. |
| Sectors | Agriculture Food and Drink |
| Description | The partner company has used them to improve the efficiency of breeding hybrid oilseed rape cultivars. |
| First Year Of Impact | 2015 |
| Sector | Agriculture, Food and Drink |
| Impact Types | Economic |
| Description | Predicting the performance of hybrid rapeseed |
| Organisation | Limagrain |
| Country | France |
| Sector | Private |
| PI Contribution | We will be undertaking computational analyses to investigate |
| Collaborator Contribution | Our partners will produce hybrid plant and measure their performance (the combining ability of the parent accessions). |
| Impact | Too early. |
| Start Year | 2018 |