Macrophage Molecular Biology
Lead Research Organisation:
Roslin Institute
Department Name: UNLISTED
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
This project is concerned with establishing procedures to isolated and characterise macrophages from bone marrow and blood of the pig, test responses of these cells to macrophage colony-stimulating factor (CSF-1) and microbial stimuli, and establishing ways of manipulating their gene expression using lentiviruses.
Planned Impact
unavailable
Organisations
People |
ORCID iD |
| David Hume (Principal Investigator) |
Publications
Fairbairn L
(2011)
The mononuclear phagocyte system of the pig as a model for understanding human innate immunity and disease.
in Journal of leukocyte biology
Kapetanovic R
(2012)
Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
in Journal of immunology (Baltimore, Md. : 1950)
Kapetanovic R
(2013)
The impact of breed and tissue compartment on the response of pig macrophages to lipopolysaccharide.
in BMC genomics
Pridans C
(2014)
Lentiviral vectors containing mouse Csf1r control elements direct macrophage-restricted expression in multiple species of birds and mammals.
in Molecular therapy. Methods & clinical development
| Description | This award enabled us to develop a simple culture system to support the production of macrophages from pig bone marrow using CSF1. The method has been widely applied by our own and other groups in Roslin and elsewhere and extended to other species. |
| Exploitation Route | This was a method development project, and the method has been published. We have also produced and commercialised through BioRad, the actor required to grow macrophages (CSF1) |
| Sectors | Agriculture Food and Drink Pharmaceuticals and Medical Biotechnology |