Plant stress responses in the rumen
Lead Research Organisation:
Aberystwyth University
Department Name: IBERS
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Technical Summary
At least half of the plant cells ingested by ruminants are viable and capable of responding to the multiple and simultaneous stresses they are suddenly exposed to (anaerobic, 39oC, invasive microbial population). These responses culminate in autolytic cell death in both the presence and absence of micro-organisms. This work involves detailed analysis of the biochemical and gene expression response of model and forage crop plants to understand the regulation of autolysis in ingested forage in the rumen, with particular emphasis on proteolysis. This will enable the identification of plant-based metabolic and genetic control points that could be amenable to manipulation and so be exploited to alter rumen ecosystem outputs.
Planned Impact
unavailable
Publications
Cheng YF
(2009)
Diversity and activity of enriched ruminal cultures of anaerobic fungi and methanogens grown together on lignocellulose in consecutive batch culture.
in Bioresource technology
Edwards J
(2008)
Characterization of the dynamics of initial bacterial colonization of nonconserved forage in the bovine rumen
in FEMS Microbiology Ecology
Edwards J
(2008)
Dynamics of initial colonization of nonconserved perennial ryegrass by anaerobic fungi in the bovine rumen Initial colonization of forage by ruminal anaerobic fungi
in FEMS Microbiology Ecology
Edwards JE
(2008)
Advances in microbial ecosystem concepts and their consequences for ruminant agriculture.
in Animal : an international journal of animal bioscience
Edwards JE
(2008)
Adaptation to flavomycin in the ruminal bacterium, Prevotella bryantii.
in Journal of applied microbiology
Huws SA
(2009)
Rumen protozoa are rich in polyunsaturated fatty acids due to the ingestion of chloroplasts.
in FEMS microbiology ecology
Huws SA
(2010)
Forage type and fish oil cause shifts in rumen bacterial diversity.
in FEMS microbiology ecology
Kim E
(2009)
Dietary Transformation of Lipid in the Rumen Microbial Ecosystem
in Asian-Australasian Journal of Animal Sciences
| Description | Evidence of successional biology of colonisation during feed degradation. Identification of feed-related colonisation patterns, evidence of biofilm formation in very early stages post-ingestion. |
| Exploitation Route | Underpinned current next generation detailed analysis of rumen metagenome as relating to the functional rumen. |
| Sectors | Agriculture Food and Drink Education Environment |
| Description | BBSRC responsive mode Genomic selection of sheep |
| Amount | £411,076 (GBP) |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2012 |
| End | 03/2015 |
| Description | DEFRA Nitrogen |
| Amount | £1,113,737 (GBP) |
| Funding ID | AC0122 |
| Organisation | Department For Environment, Food And Rural Affairs (DEFRA) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2012 |
| End | 09/2018 |
| Description | EU CANTOGETHER |
| Amount | £194,976 (GBP) |
| Funding ID | 289328 |
| Organisation | European Commission |
| Sector | Public |
| Country | Belgium |
| Start | 02/2012 |
| End | 01/2016 |
| Description | EU MULTISWARD |
| Amount | £79,238 (GBP) |
| Funding ID | 244983 |
| Organisation | European Commission |
| Sector | Public |
| Country | Belgium |
| Start | 03/2010 |
| End | 02/2014 |
| Description | EU-SOLID |
| Amount | £760,161 (GBP) |
| Funding ID | 266367 |
| Organisation | European Commission |
| Sector | Public |
| Country | Belgium |
| Start | 03/2011 |
| End | 02/2016 |
| Description | IBERS Studentship Chloroplastic phenolic oxidases |
| Amount | £50,000 (GBP) |
| Organisation | University of Wales |
| Department | Institute of Biological Environmental and Rural Sciences (IBERS) |
| Sector | Academic/University |
| Country | United Kingdom |
| Start | 09/2010 |
| End | 09/2013 |
| Description | ISPG Rumen Systems Biology |
| Amount | £3,375,500 (GBP) |
| Funding ID | BBS/E/W/10964A01 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 03/2012 |
| End | 03/2017 |
| Description | International Workshop |
| Amount | £10,630 (GBP) |
| Funding ID | BB/1025212/1 |
| Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
| Sector | Public |
| Country | United Kingdom |
| Start | 08/2011 |
| End | 10/2011 |
| Description | Postgraduate studentship Protein utilisation in forage fed animals |
| Amount | £52,246 (GBP) |
| Organisation | EBLEX |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 09/2009 |
| End | 09/2012 |
| Description | Postgraduate studentship genetic basis for slow plant-mediated proteolysis in Festulolium hybrids |
| Amount | £52,246 (GBP) |
| Organisation | EBLEX |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 09/2010 |
| End | 09/2013 |
| Description | Stapledon Fund Sumit Dagar |
| Amount | £5,800 (GBP) |
| Organisation | Stapledon Memorial Trust |
| Sector | Charity/Non Profit |
| Country | United Kingdom |
| Start | 03/2012 |
| End | 09/2012 |
| Description | International partnership agreement, Bulgaria |
| Organisation | Bulgarian Academy of Sciences |
| Country | Bulgaria |
| Sector | Academic/University |
| PI Contribution | Visit by Dr Kingston-Smith to the laboratory of Prof K Demirevska (Sofia, Bulgaria) to design the experimental plan in the subject area "The role of redox state on regulation of proteolysis under anoxia". Subsequent analysis of samples sent from Bulgaria to researchers in Aberystwyth. |
| Collaborator Contribution | PI and Post-doctoral input to joint research project to establish and perform experiment, undertake analyses where possible. |
| Impact | Simova-Stoilova LP, Demirevska KN, Kingston-Smith AH, Feller U. (2011) Involvement of the leaf antioxidant system in the response to soil flooding in two Trifolium genotypes differing in their tolerance to waterlogging. Plant Science.183: 43-49. |
| Start Year | 2010 |
| Description | Prevotella microarray |
| Organisation | University of Illinois at Urbana-Champaign |
| Country | United States |
| Sector | Academic/University |
| PI Contribution | Visit by Joan Edwards to laboratory of Prof Rod Mackie to carry out experimentation with Prevotella microarray available there. |
| Collaborator Contribution | Access to facilities, intellectual input into experimental design and analysis. |
| Impact | Conference presentations: Yeoman, C.J., Edwards, J.E., Cann, I.K., Morrison,M., Kingston-Smith, A.H. and Mackie, R.I. (2009) Transcriptional Analyses of Carbohydrate Metabolism in Prevotella ruminicola. Gordon Research Conference: Cellulosomes, Cellulases & Other Carbohydrate Modifying Enzymes Carl J. Yeoman, Joan Edwards, Alison Kingston-Smith and Roderick I. Mackie (2102) Transcriptional responses of Prevotella ruminicola reflect spatial and temporal differences in available nutrients from fresh perennial ryegrass. Rowett-INRA Gut Microbiology Symposium. |
| Start Year | 2009 |
| Description | Dairy Science Forum |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Talk by Alison Kingston-Smith on The role of plant enzymes in rumen proteolysis, presented new research information stimulating discussion about application and future plans. Continued discussion on new forage varieties, promoted engagement of researchers and community. |
| Year(s) Of Engagement Activity | 2008 |
| Description | Women in Food and Farming |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Public/other audiences |
| Results and Impact | Presentation to WI group Women in Food and Farming as part of a workshop on GM crops. Audience included interested members of public, politicians, academics and activists. Media coverage (local paper) of the event. Audience tended to be anti-GM so main impact was the ability to have a discussion of contrasting views. |
| Year(s) Of Engagement Activity | 2011 |