Synthesis Of Mucin Type O-Glycans Via Ionic Catch And Release Methodology. Towards The Automated Synthesis Of Oligosaccharides.
Lead Research Organisation:
University of Bristol
Department Name: Chemistry
Abstract
Carbohydrates have emerged as important factors of molecular recognition in biology and medicine, however they are still difficult to synthesize. Combinatorial approaches to prepare diverse libraries of oligosaccharides are greatly lacking and progress in glycobiology research has been hindered by having to rely on either isolated materials or lengthy target-oriented syntheses of complex oligomers. An automated synthesis of oligosaccharides would provide access to diversity-orientated oligosaccharide libraries to enhance not only glycobiology research, but also carbohydrate-based drug discovery. In this proposal we have outlined an ambitious and wide ranging programme aimed at synthesizing oligosaccharide components of the mucin family of proteins, for which the function and properties have yet to be investigated. In recent years, reports have emerged identifying some key candidate mucin oligosaccharide structures found throughout the intestinal track, however none are commercially available and, as far as we are aware, have not been chemically synthesized. The synthesis of defined fragments of this class of glycans will be critical to help us understand their biological and disease implications and will provide pure materials for biological exploration, which will subsequently have significant impact on glycoarray screening, diagnostic tests, vaccine synthesis and glycan-based therapeutics. Concurrently, we aim to develop new methodology for the general synthesis and rapid purification of oligosaccharides. The proposed technique is based on ionic pair interactions between an immobilization media (ionic liquid) and a suitably tagged carbohydrate that would enable catch and release of the required product, simplifying greatly purification. In another aspect, we will explore the scope of ionic liquids (ILs) as dual solvent/catalysts for glycosylation reactions that can be recycled. It is our long term objective to apply this methodology to the automated synthesis of complex oligosaccharide structures, for which effective automated techniques are lacking.
Organisations
Publications
Galan M
(2011)
[R 4 N] [AOT]: A Surfactant Ionic Liquid as a Mild Glycosylation Promoter
in Journal of Carbohydrate Chemistry
Galan M
(2009)
[bmim][OTf]: a versatile room temperature glycosylation promoter
in Tetrahedron Letters
Galan MC
(2013)
Multivalent glyco(cyclo)peptides.
in Chemical Society reviews
Galan MC
(2010)
Scope and limitations of imidazolium-based ionic liquids as room temperature glycosylation promoters.
in Carbohydrate research
Galan MC
(2010)
[bmim][OTf] as co-solvent/promoter in room temperature reactivity-based one-pot glycosylation reactions.
in Chemical communications (Cambridge, England)
Galan MC
(2010)
Ionic-liquid-based catch and release mass spectroscopy tags for enzyme monitoring.
in Chemical communications (Cambridge, England)
Galan MC
(2010)
Ionic liquids in oligosaccharide synthesis: towards mucin-type glycan probes.
in Biochemical Society transactions
Galan MC
(2011)
Carbohydrate chemistry in drug discovery.
in Organic & biomolecular chemistry
Galan MC
(2011)
Carbohydrate chemistry in drug discovery.
in Organic & biomolecular chemistry
Ghirardello M
(2022)
Recent applications of ionic liquid-based tags in glycoscience.
in Carbohydrate research
Description | A method to stream-line the synthesis of oligosaccharides was developed and disease relevant o-glycans were prepared using the methodology. |
Exploitation Route | The methods could be used by others to make other similar molecules |
Sectors | Chemicals,Other |
Description | EPSRC |
Amount | £920,000 (GBP) |
Funding ID | EP/J002542/1 |
Organisation | Engineering and Physical Sciences Research Council (EPSRC) |
Sector | Public |
Country | United Kingdom |
Start | |
End | 08/2017 |
Description | European Union Framework 7 |
Amount | £143,951 (GBP) |
Funding ID | ESR 236061 |
Organisation | European Commission |
Department | Seventh Framework Programme (FP7) |
Sector | Public |
Country | European Union (EU) |
Start |
Description | European Union Framework 7 |
Amount | £143,951 (GBP) |
Funding ID | ESR 236061 |
Organisation | European Commission |
Department | Seventh Framework Programme (FP7) |
Sector | Public |
Country | European Union (EU) |
Start |
Description | Novartis |
Amount | £36,000 (GBP) |
Funding ID | 30090 |
Organisation | Novartis |
Sector | Private |
Country | Global |
Start | 10/2009 |
End | 04/2013 |
Description | Royal Society of London |
Amount | £373,000 (GBP) |
Funding ID | CHEM RK6968 |
Organisation | The Royal Society |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start |
Description | Royal Society of London |
Amount | £373,000 (GBP) |
Funding ID | CHEM RK6968 |
Organisation | The Royal Society |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start |
Description | Outreach lectures for Bristol Chem Labs to secondary schools |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Schools |
Results and Impact | over 100 students attended seminars about the role of carbohydrates in life which aparked loads of questions an interest. As a result of my seminars, I have been invited to visit schools to engage with their cohort |
Year(s) Of Engagement Activity | 2012,2013,2014,2015,2016,2017 |