Engineering stable calibration standards for biomedical research
Lead Research Organisation:
University of Oxford
Department Name: Oxford Chemistry
Abstract
Work by Davis has demonstrated the feasibility of new manufacturing routines for sitespecificmodification of proteins. This project aims to establish whether these newmanufacturing processes represent a means of manufacturing precise protein calibrationstandard products offering advantages in terms of product specification, stability, processflexibility, economics and scaling. This will find application in enabling first-in-classquantitative immunoassays and standards for mass spectrometry.
Publications
Aljabali A. A. A.
(2014)
Multimeric microparticles of iron oxide (mMPIO) for dual modality molecular imaging with CT & MRI
in EUROPEAN HEART JOURNAL
Baboo S
(2014)
Most human proteins made in both nucleus and cytoplasm turn over within minutes.
in PloS one
Backus KM
(2014)
The three Mycobacterium tuberculosis antigen 85 isoforms have unique substrates and activities determined by non-active site regions.
in The Journal of biological chemistry
Backus KM
(2011)
Uptake of unnatural trehalose analogs as a reporter for Mycobacterium tuberculosis.
in Nature chemical biology
Bagi Zsolt
(2013)
Microparticles bearing RGD peptide promote integrin avß3-dependent platelet adhesion in isolated, pressurized cerebral artery
in FASEB JOURNAL
Barry C
(2013)
'Naked' and Hydrated Conformers of the Conserved Core Pentasaccharide of N-linked Glycoproteins and Its Building Blocks
in Journal of the American Chemical Society
Barry CS
(2011)
ESI-MS assay of M. tuberculosis cell wall antigen 85 enzymes permits substrate profiling and design of a mechanism-based inhibitor.
in Journal of the American Chemical Society
Bernardes GJ
(2011)
Site-selective traceless Staudinger ligation for glycoprotein synthesis reveals scope and limitations.
in Chembiochem : a European journal of chemical biology
Bernardes Goncalo J. L.
(2012)
Chemical site-selective protein modification: Development of a traceless vascular targeting ADC for cancer therapy
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Bernardes Goncalo J. L.
(2012)
Building well-defined glycoproteins
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
| Description | We worked a chemical method for building proteins that act as 'molecular display units'. By using them to display important peptide motifs, we found a way of getting those peptides to be recognised by antibodies. This, in turn, allowed our commercial partners to improve the calibration of their own antibodies, making their products more precise (and hence valuable). In turn, these 'better' calibrated antibodies are allowing more precise and quantitative use of antibodies as widespread 'reagents' in biological science. |
| Exploitation Route | We think that the development of 'better' antibodies will have widespread benefits to improving their use. Beyond that we can envisage using our 'display units' to display all sorts of things in biology, allowing even the design of molecular rulers, for instance. |
| Sectors | Chemicals,Pharmaceuticals and Medical Biotechnology |
| URL | http://users.ox.ac.uk/~dplb0149/ |
| Description | BGD and group members have appeared on the radio, television, (BBD, Channel 5), science festivals around the world (Cheltenham, Kent, Edinburgh, Times Lit., Sydney) describing this work. We have given talks in schools to inspire the next generation. The commercial partners on this work (Badrilla Ltd) have licensed the approach and this increasing their development and growth. |
| First Year Of Impact | 2011 |
| Sector | Chemicals,Healthcare,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Cultural,Societal,Economic |
| Company Name | Glycoform Ltd |
| Description | drug delivery and glycoprotein specialist; biopharmaceuticals |
| Impact | Employed >20 people over 10 years and provided a model for how synthetic protein drugs might be constructed and used. The technology for this company has now been used by major US companies. |
| Website | http://isis-innovation.com/news/glycoform-ltd-improve-drug-delivery/ |