Scale up of optical fractionation for bio-processing
Lead Research Organisation:
Heriot-Watt University
Department Name: Sch of Engineering and Physical Science
Abstract
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Organisations
People |
ORCID iD |
| Nicholas Willoughby (Principal Investigator) |
Publications
Guzniczak E
(2017)
High-throughput assessment of mechanical properties of stem cell derived red blood cells, toward cellular downstream processing.
in Scientific reports
O'Mahoney P
(2016)
Acoustic trapping in bubble-bounded micro-cavities
in Optofluidics, Microfluidics and Nanofluidics
Thalhammer G
(2016)
Acoustic force mapping in a hybrid acoustic-optical micromanipulation device supporting high resolution optical imaging.
in Lab on a chip
Willoughby N
(2016)
A scalable label-free approach to separate human pluripotent cells from differentiated derivatives
in Biomicrofluidics
| Description | 1) Discovered a new hybrid optical/acoustic cell sorting mechanism (patent filed) particularly suited for the removal of rare cell species within a high throughput flow. 2) Development of generic strategies for scale up of cell sorting, for example through the use of sorting from an equilibrium state to give robust sorting. 3) Through a new collaboration established with the Medical University of Innsbruck we have developed an optical technique for characterising acoustic trapping which can be used for calibrating acoustic force spectroscopy. 4) Developed disposable microfluidic test chambers through the use of our pioneering transparent ultrasonic transducers. This approach is now being adopted internationally as a method for combining optical and acoustic manipulation and imaging. |
| Exploitation Route | Key scale-up parameters and protocols identified and developed during this work can be used by others to establish protocols for cell purification at large scale. This will be absolutely crucial to realise the huge potential of allogeneic cell therapies. Most likely route to take forward our findings is through out collaborative partnership, now known as "Novosang": http://novosang.co.uk/approach |
| Sectors | Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| Description | Please see the impact description provided for EP/L022370/1 |
| Description | The Future of Cell Therapies - an Interdisciplinary Workshop |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Postgraduate students |
| Results and Impact | Interdisciplinary collaborative workshop with students from 3 different Scottish MSc programs (regenerative medicine at Edinburgh, industrial biotechnology at Strathclyde and biomedical technology at Dundee) looking to work together and use cross-disciplinary skillsets to solve future cell therapy challenges |
| Year(s) Of Engagement Activity | 2018 |