'Last step' enzymatic [18F]-labelling of peptides for Positron Emission Tomography (PET)
Lead Research Organisation:
University of Aberdeen
Department Name: Sch of Medicine, Medical Sci & Nutrition
Abstract
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People |
ORCID iD |
| Matteo Zanda (Principal Investigator) |
Publications
Zhang Q
(2016)
Last-Step Enzymatic [(18) F]-Fluorination of Cysteine-Tethered RGD Peptides Using Modified Barbas Linkers.
in Chemistry (Weinheim an der Bergstrasse, Germany)
| Description | We have validated an enzymatic last-step [18F]radiofluorination of bioactive peptides for biomedical PET imaging |
| Exploitation Route | The technology could be used by other PET centres in UK and overseas for producing PET tracers |
| Sectors | Healthcare,Pharmaceuticals and Medical Biotechnology |
| Title | FDR-based prosthetic groups for late stage radiofluorination |
| Description | We have modified a cysteine-containing RGD peptide with a fluorinase-recognized chloroadenosine for fluorinase mediated radiolabeling with the fluorine-18 isotope. The recently introduced Barbas bioconjugation strategy was variously modified to improve solubility. The resulting adduct retained high affinity to cancer-cell relevant avb3-integrins. This was used as a proof-of-concept example to demonstrate the feasibility of using fluorinase for achieving late-stage radiofluorinations of biologically important molecules for the development of novel PET diagnostics and theranostics. |
| Type Of Material | Technology assay or reagent |
| Provided To Others? | No |
| Impact | This could become a highly valuable method for achieving radiofluorination of biologically important small molecules, peptides and proteins (including antibodies) in aqueous solution using an enzyme. It could have a major impact in PET radiochemistry and imaging. We are currently testing full scope and limitations of this exciting new technology. |
| Description | Collaboration with Prof. David O'Hagan |
| Organisation | University of St Andrews |
| Department | School of Chemistry St Andrews |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | PET radiochemistry, tracer design and Imaging using fluorinase enzyme and Fluoro-deoxy-ribose |
| Collaborator Contribution | Chemistry and tracer design with fluorinase enzyme |
| Impact | Publications and a second EPSRC grant (starting March 2015) |
| Start Year | 2009 |