The CHELL : A Bottom-Up approach to in vitro and in silico Minimal Life-like Constructs
Lead Research Organisation:
University of Oxford
Department Name: Oxford Chemistry
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
Organisations
Publications
Davis Ben
(2012)
Sugars and proteins
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Guimaraes Andreia M. R.
(2011)
Glycosylation enhances selectivity of novobiocin and alters antibacterial mechanism
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Chooi K. Phin
(2014)
Giving p38a kinase a jump-start: p38a Activation induced by chemical modification
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Floyd Nicola
(2009)
ORGN 260-An unexpected photo-induced site-selective protein cleavage reaction
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Chalker Justin M.
(2011)
Suzuki-Miyaura cross-coupling on protein substrates using a novel palladium-pyrimidine catalyst
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Hong Sung You
(2009)
Functionalization of single walled carbon nanotubes with carbohydrates
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Xue Xuan
(2011)
Synthetic polymers for interference with bacterial communication
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Bernardes Goncalo J. L.
(2012)
Building well-defined glycoproteins
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Spicer Christopher D.
(2013)
Suzuki biology: Palladium mediated protein modification
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Bernardes Goncalo J. L.
(2012)
Chemical site-selective protein modification: Development of a traceless vascular targeting ADC for cancer therapy
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Chalker Justin M.
(2011)
Allyl sulfides enable olefin metathesis in water and on proteins
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Chalker Justin M.
(2011)
Reaction engineering for protein modification
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Lee Seung Seo
(2011)
Biosynthesis of nucleoside antibiotic tunicamycin proceeds via a unique
exo-glycal intermediate
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Davis Benjamin G.
(2009)
Sugar and proteins: Strategies in synthetic biology
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Boutureira Omar
(2012)
Chemical site-selective radiolabelling of proteins using fluorosugars
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Parry Alison L.
(2009)
Glycopolymer-functionalized gold nanoparticles: A new strategy toward synthetic anticancer vaccines
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Simons John P.
(2011)
Gas phase spectroscopy of hydrated carbohydrates: The anomeric effect laid bare
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Chalker JM
(2011)
A "tag-and-modify" approach to site-selective protein modification.
in Accounts of chemical research
Schiffner T.
(2013)
Focusing Antibody Responses to Specific Protein Surfaces Through Site-Selective Glycan Addition
in AIDS RESEARCH AND HUMAN RETROVIRUSES
Schiffner Torben
(2014)
Refocussing Antibody Responses by Chemical Modification of Vaccine Antigens
in AIDS RESEARCH AND HUMAN RETROVIRUSES
Dumas A
(2013)
Self-liganded Suzuki-Miyaura coupling for site-selective protein PEGylation.
in Angewandte Chemie (International ed. in English)
French AC
(2009)
High-purity discrete PEG-oligomer crystals allow structural insight.
in Angewandte Chemie (International ed. in English)
Kreizman R
(2009)
Core-shell PbI2@WS2 inorganic nanotubes from capillary wetting.
in Angewandte Chemie (International ed. in English)
Errey JC
(2010)
Mechanistic insight into enzymatic glycosyl transfer with retention of configuration through analysis of glycomimetic inhibitors.
in Angewandte Chemie (International ed. in English)
Grayson EJ
(2011)
A coordinated synthesis and conjugation strategy for the preparation of homogeneous glycoconjugate vaccine candidates.
in Angewandte Chemie (International ed. in English)
Chalker JM
(2012)
Conversion of cysteine into dehydroalanine enables access to synthetic histones bearing diverse post-translational modifications.
in Angewandte Chemie (International ed. in English)
Xue X
(2011)
Synthetic polymers for simultaneous bacterial sequestration and quorum sense interference.
in Angewandte Chemie (International ed. in English)
Floyd N
(2009)
Thiyl glycosylation of olefinic proteins: S-linked glycoconjugate synthesis.
in Angewandte Chemie (International ed. in English)
Lercher L
(2013)
DNA modification under mild conditions by Suzuki-Miyaura cross-coupling for the generation of functional probes.
in Angewandte Chemie (International ed. in English)
Davis BG
(2009)
The linear assembly of a pure glycoenzyme.
in Angewandte Chemie (International ed. in English)
Boutureira O
(2012)
Selenenylsulfide-linked homogeneous glycopeptides and glycoproteins: synthesis of human "hepatic Se metabolite A".
in Angewandte Chemie (International ed. in English)
Yamamoto K
(2012)
Creation of an a-mannosynthase from a broad glycosidase scaffold.
in Angewandte Chemie (International ed. in English)
Seebach D
(2009)
Polymer backbone conformation--a challenging task for database information retrieval.
in Angewandte Chemie (International ed. in English)
Lin Y
(2010)
The allylic chalcogen effect in olefin metathesis
in Beilstein Journal of Organic Chemistry
Schuster HJ
(2015)
Chain-growth polyglycosylation: synthesis of linker-equipped mannosyl oligomers.
in Carbohydrate research
Russell D
(2009)
Site-selective chemical protein glycosylation protects from autolysis and proteolytic degradation.
in Carbohydrate research
Monrad R
(2014)
Dissecting the reaction of Phase II metabolites of ibuprofen and other NSAIDS with human plasma protein
in Chem. Sci.
Allman SA
(2009)
Potent fluoro-oligosaccharide probes of adhesion in Toxoplasmosis.
in Chembiochem : a European journal of chemical biology
Bernardes GJ
(2011)
Site-selective traceless Staudinger ligation for glycoprotein synthesis reveals scope and limitations.
in Chembiochem : a European journal of chemical biology
Lin YA
(2009)
Olefin metathesis for site-selective protein modification.
in Chembiochem : a European journal of chemical biology
Chalker JM
(2009)
Enabling olefin metathesis on proteins: chemical methods for installation of S-allyl cysteine.
in Chemical communications (Cambridge, England)
Lo Conte M
(2011)
Multi-molecule reaction of serum albumin can occur through thiol-yne coupling.
in Chemical communications (Cambridge, England)
Spicer CD
(2011)
Palladium-mediated site-selective Suzuki-Miyaura protein modification at genetically encoded aryl halides.
in Chemical communications (Cambridge, England)
Boutureira O
(2011)
Direct radiolabelling of proteins at cysteine using [18F]-fluorosugars.
in Chemical communications (Cambridge, England)
Spicer CD
(2013)
Rewriting the bacterial glycocalyx via Suzuki-Miyaura cross-coupling.
in Chemical communications (Cambridge, England)
Boutureira O
(2010)
Fluoroglycoproteins: ready chemical site-selective incorporation of fluorosugars into proteins.
in Chemical communications (Cambridge, England)
Vallée MR
(2016)
Ready display of antigenic peptides in a protein 'mimogen'.
in Chemical communications (Cambridge, England)
Rutte RN
(2014)
Modification of fulleropyrazolines modulates their cleavage by light.
in Chemical communications (Cambridge, England)
Pérez-Victoria I
(2015)
Glycosyldiselenides as lectin ligands detectable by NMR in biofluids.
in Chemical communications (Cambridge, England)
Pérez-Victoria I
(2009)
Saturation transfer difference NMR reveals functionally essential kinetic differences for a sugar-binding repressor protein.
in Chemical communications (Cambridge, England)
| Description | We found that we were not only able to make an artificial chemical cell (something we called the 'chell') but that we could also create sufficient information from its metabolism for it to communicate with living (bacterial cells). This 'conversation' between artificial cells and living cells highlights that our notions of cellularity (and life) can be examined through fundamental experiments. |
| Exploitation Route | These ideas have provoked various communities that examine cells right down to those who are interested in the emergence of complexity and even studying the origins of life. It provoked an analysis of the use of 'chemical cells' as potential 'living drugs', for example - see https://vimeo.com/10274649. |
| Sectors | Education,Pharmaceuticals and Medical Biotechnology |
| URL | http://users.ox.ac.uk/~dplb0149/index.html |
| Description | BGD and group members have appeared on the radio, television, (BBD, Channel 5), newspapers (Times, New Scientist) science festivals around the world (Cheltenham, Kent, Edinburgh, Times Lit., Sydney) describing this work. We have given talks in schools to inspire the next generation. This work has been widely featured as one of the very first SynBio grants and that led to an expansion of the UK's SynBio community. It has also provoked a community of those more broadly interested in these concepts, including designers and artists - see https://vimeo.com/10274649 or http://www.daisyginsberg.com/work/synthesis-exchange-laboratory |
| First Year Of Impact | 2009 |
| Sector | Chemicals,Creative Economy,Education,Pharmaceuticals and Medical Biotechnology,Other |
| Impact Types | Cultural,Societal,Economic,Policy & public services |
| Company Name | Glycoform Ltd |
| Description | drug delivery and glycoprotein specialist; biopharmaceuticals |
| Impact | Employed >20 people over 10 years and provided a model for how synthetic protein drugs might be constructed and used. The technology for this company has now been used by major US companies. |
| Website | http://isis-innovation.com/news/glycoform-ltd-improve-drug-delivery/ |