Engineering stable calibration standards for biomedical research
Lead Research Organisation:
University of Oxford
Department Name: Oxford Chemistry
Abstract
Work by Davis has demonstrated the feasibility of new manufacturing routines for sitespecificmodification of proteins. This project aims to establish whether these newmanufacturing processes represent a means of manufacturing precise protein calibrationstandard products offering advantages in terms of product specification, stability, processflexibility, economics and scaling. This will find application in enabling first-in-classquantitative immunoassays and standards for mass spectrometry.
Publications
Yamamoto K
(2012)
Creation of an a-mannosynthase from a broad glycosidase scaffold.
in Angewandte Chemie (International ed. in English)
Xue Xuan
(2011)
Synthetic polymers for interference with bacterial communication
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Xue X
(2011)
Synthetic polymers for simultaneous bacterial sequestration and quorum sense interference.
in Angewandte Chemie (International ed. in English)
Wyszynski FJ
(2012)
Biosynthesis of the tunicamycin antibiotics proceeds via unique exo-glycal intermediates.
in Nature chemistry
Wright TH
(2016)
From Chemical Mutagenesis to Post-Expression Mutagenesis: A 50 Year Odyssey.
in Angewandte Chemie (International ed. in English)
Wright T
(2016)
Von der chemischen Mutagenese zur Postexpressions-Mutagenese: eine 50 Jahre währende Odyssee
in Angewandte Chemie
Wang LX
(2013)
Realizing the Promise of Chemical Glycobiology.
in Chemical science
Vallée MR
(2016)
Ready display of antigenic peptides in a protein 'mimogen'.
in Chemical communications (Cambridge, England)
Spicer Christopher D.
(2013)
Suzuki biology: Palladium mediated protein modification
in ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
Spicer CD
(2011)
Palladium-mediated site-selective Suzuki-Miyaura protein modification at genetically encoded aryl halides.
in Chemical communications (Cambridge, England)
| Description | We worked a chemical method for building proteins that act as 'molecular display units'. By using them to display important peptide motifs, we found a way of getting those peptides to be recognised by antibodies. This, in turn, allowed our commercial partners to improve the calibration of their own antibodies, making their products more precise (and hence valuable). In turn, these 'better' calibrated antibodies are allowing more precise and quantitative use of antibodies as widespread 'reagents' in biological science. |
| Exploitation Route | We think that the development of 'better' antibodies will have widespread benefits to improving their use. Beyond that we can envisage using our 'display units' to display all sorts of things in biology, allowing even the design of molecular rulers, for instance. |
| Sectors | Chemicals,Pharmaceuticals and Medical Biotechnology |
| URL | http://users.ox.ac.uk/~dplb0149/ |
| Description | BGD and group members have appeared on the radio, television, (BBD, Channel 5), science festivals around the world (Cheltenham, Kent, Edinburgh, Times Lit., Sydney) describing this work. We have given talks in schools to inspire the next generation. The commercial partners on this work (Badrilla Ltd) have licensed the approach and this increasing their development and growth. |
| First Year Of Impact | 2011 |
| Sector | Chemicals,Healthcare,Pharmaceuticals and Medical Biotechnology |
| Impact Types | Cultural,Societal,Economic |
| Company Name | Glycoform Ltd |
| Description | drug delivery and glycoprotein specialist; biopharmaceuticals |
| Impact | Employed >20 people over 10 years and provided a model for how synthetic protein drugs might be constructed and used. The technology for this company has now been used by major US companies. |
| Website | http://isis-innovation.com/news/glycoform-ltd-improve-drug-delivery/ |