Post-Translational Modifications in the Radiation Damage Response
Lead Research Organisation:
University of Oxford
Department Name: UNLISTED
Abstract
DNA replication and DNA repair are two essential processes for genome amplification and stability. Defects in these two processes lead to genome instability and various human diseases such as premature aging and cancer. We have recently identified that the ubiquitin dependent AAA ATPase p97 plays an essential role in the regulation of DNA replication and DNA repair. The specificity of p97 in the regulation of DNA replication and DNA repair is governed by p97 cofactors. Now, we aim to identify and characterise the p97 system (p97/VCP + cofactors) involved in the regulation of DNA replication and DNA repair. Our current results demonstrate that mutations in p97 cofactors lead to cancer and premature aging in humans. Further identification and characterisation of the p97 system will help us to understand why mutations in p97 cofactors lead to cancer. We will use biochemical and cell biological approaches to delineate molecular mechanisms of the p97 system in DNA replication and repair. Given that cancer cells divide much faster than the majority of human cells and have altered metabolism, which leads to elevated DNA damage, the p97 system has emerged as an attractive drug target for cancer therapy. This award aims to demonstrate a new concept, which is based on the hypothesis that the survival and chemoresistance of many cancers depends on the p97 system, and will pave the way for identification of potential p97 system inhibitors for cancer therapy.
Technical Summary
Posttranslational modifications (PTMs) including ubiquitination and SUMOylation are essential for the DNA damage response (DDR) and DNA replication. The local accumulation of these proteinaceous PTMs at sites of DNA damage and at sites of DNA replication is essential for accumulation and disassembly of DDR and DNA replication proteins and thus paramount for genome stability. Defects in ubiquitin (Ub) and SUMO signals lead to various human diseases including neurodegeneration, premature aging and cancer. The main objective of the group is to decipher the ubiquitin (Ub) and SUMO signals in DDR after ionising radiation (IR) and in DNA replication. To address this objective we study the central component of the ubiquitin-proteasome system (UPS), the AAA+ ATPase p97 (also known as VCP in mammals or Cdc48 in lower eukaryotes). p97 is the Ub-dependent molecular chaperone that, in association with its cofactors (around 30 are known), controls the rate and dynamics of Ub and SUMO dependent protein turnover and degradation. The p97 system (p97+cofactors) is essential for various processes in chromatin biology. My work has identified the role of p97 associated with chromatin for the first time and opened a new arena of research, namely the removal and degradation of chromatin-associated proteins. The Group’s work has further discovered the essential role of p97 in DNA double strand break (DSB) repair after IR, and a direct link between mutations in a component of the p97 system and accelerated aging and cancer in humans. Moreover, we have just identified that p97 cofactor SPARTAN is a DNA-dependent metalloprotease for repair of DNA-protein crosslinks, under-investigated DNA lesions associated with premature ageing and cancer. Based on these findings, we have evidence that a specialised DNA repair pathway exists, which removes DNA-protein crosslinks in vertebrates. By using quantitative proteomic approaches we have identified three p97 sub-complexes, which associate with chromatin after IR. Now, we aim to use biochemical and cell biological approaches in different human normal and cancer cell lines to characterise the p97 system in genome stability. Specifically, we will focus on: (i) characterisation of SPARTAN enzymatic activity and identification of its substrates, and (ii) characterisation of three p97-chromatin associated sub-complexes and how these sub-complexes orchestrate DNA replication and DNA double strand break repair after IR. As components of both the UPS (proteasome inhibitor bortezomib -Velcade®) and DDR (PARP inhibitor olaparib - Lynparza®) are druggable targets and approved cancer therapeutics, investigating the p97 system is of importance to extend our understanding of carcinogenesis and developing novel cancer therapies.
Organisations
- University of Oxford (Lead Research Organisation)
- UNIVERSITY OF OXFORD (Collaboration)
- EVOTEC (Collaboration)
- Danish Cancer Society (Collaboration)
- University of Sheffield (Collaboration)
- Institute of Cancer Research UK (Collaboration)
- University of La Laguna (Collaboration)
- National Institutes of Health (NIH) (Collaboration)
- Russian Academy of Sciences (Collaboration)
- Tel Aviv University (Collaboration)
- University Hospital Tuebingen (Collaboration)
People |
ORCID iD |
Publications
Baranes-Bachar K
(2018)
The Ubiquitin E3/E4 Ligase UBE4A Adjusts Protein Ubiquitylation and Accumulation at Sites of DNA Damage, Facilitating Double-Strand Break Repair.
in Molecular cell
Bolland H
(2021)
Links between the unfolded protein response and the DNA damage response in hypoxia: a systematic review.
in Biochemical Society transactions
Chen X
(2018)
Interplay between base excision repair protein XRCC1 and ALDH2 predicts overall survival in lung and liver cancer patients.
in Cellular oncology (Dordrecht)
Fielden J
(2020)
TEX264 coordinates p97- and SPRTN-mediated resolution of topoisomerase 1-DNA adducts.
in Nature communications
Fielden J
(2018)
DNA protein crosslink proteolysis repair: From yeast to premature ageing and cancer in humans.
in DNA repair
Fielden J
(2022)
TEX264 at the intersection of autophagy and DNA repair.
in Autophagy
Halder S
(2019)
SPRTN protease and checkpoint kinase 1 cross-activation loop safeguards DNA replication.
in Nature communications
Herbert KJ
(2021)
Targeting TOPK sensitises tumour cells to radiation-induced damage by enhancing replication stress.
in Cell death and differentiation
Hume S
(2021)
The NUCKS1-SKP2-p21/p27 axis controls S phase entry
in Nature Communications
Related Projects
Project Reference | Relationship | Related To | Start | End | Award Value |
---|---|---|---|---|---|
MC_UU_00001/1 | 01/04/2017 | 31/03/2022 | £2,508,000 | ||
MC_UU_00001/2 | Transfer | MC_UU_00001/1 | 01/04/2017 | 31/03/2022 | £2,488,000 |
MC_UU_00001/3 | Transfer | MC_UU_00001/2 | 01/04/2017 | 31/05/2018 | £349,000 |
MC_UU_00001/4 | Transfer | MC_UU_00001/3 | 01/04/2017 | 31/03/2022 | £2,486,000 |
MC_UU_00001/5 | Transfer | MC_UU_00001/4 | 01/04/2017 | 30/09/2019 | £1,732,000 |
MC_UU_00001/6 | Transfer | MC_UU_00001/5 | 01/04/2017 | 31/03/2022 | £2,525,000 |
MC_UU_00001/7 | Transfer | MC_UU_00001/6 | 01/04/2017 | 31/03/2022 | £1,773,000 |
MC_UU_00001/8 | Transfer | MC_UU_00001/7 | 03/01/2019 | 31/03/2023 | £2,682,000 |
MC_UU_00001/9 | Transfer | MC_UU_00001/8 | 01/10/2019 | 31/03/2022 | £1,492,800 |
MC_UU_00001/10 | Transfer | MC_UU_00001/9 | 07/12/2020 | 31/03/2023 | £888,708 |
MC_UU_00001/11 | Transfer | MC_UU_00001/10 | 08/01/2021 | 31/03/2023 | £874,512 |
Description | Breast Cancer Now/Targeting SPRTN protease to overcome PARP inhibitor resistance in breast cancers |
Amount | £199,662 (GBP) |
Funding ID | 2019DecPR1406 |
Organisation | Breast Cancer Now |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 04/2020 |
End | 03/2023 |
Description | Characterisation of SPRTN protease in DNA replication and repair of Top1-cleavage complex |
Amount | £36,000 (GBP) |
Organisation | Nuovo Soldati Foundation for Cancer Research |
Sector | Charity/Non Profit |
Country | Switzerland |
Start | 10/2020 |
End | 09/2023 |
Description | DNA-protein crosslink repair: characterization and regulation of a novel repair machinery. |
Amount | £60,000 (GBP) |
Funding ID | ALTF 1109-2017 |
Organisation | European Molecular Biology Organisation |
Sector | Charity/Non Profit |
Country | Germany |
Start | 08/2018 |
End | 07/2020 |
Description | LAB282 Award |
Amount | £500,000 (GBP) |
Organisation | LAB 282 |
Sector | Charity/Non Profit |
Start | 10/2017 |
End | 09/2020 |
Description | The role of autophagy in reparing chemotherapy-induced DNA lesions in cancer |
Amount | £36,000 (GBP) |
Funding ID | 14548187 |
Organisation | Fonds National de la Recherche (FNR) |
Sector | Charity/Non Profit |
Country | Luxembourg |
Start | 10/2020 |
End | 09/2023 |
Title | ATX3 variants inducible cell lines |
Description | We have created doxycycline inducible HEK293 cell lines to express various ATX3 variants. |
Type Of Material | Cell line |
Year Produced | 2019 |
Provided To Others? | Yes |
Impact | -one original scientific publication |
URL | https://pubmed.ncbi.nlm.nih.gov/31613024/ |
Title | Ataxin 3-knock out HeLa, HEK293 and U2OS cell lines |
Description | We have created Ataxin 3 knock out in three human cell lines (HeLa, HEK293 and U2OS). |
Type Of Material | Cell line |
Year Produced | 2019 |
Provided To Others? | Yes |
Impact | We have directly demonstrated that Ataxin 3 is essential for DNA repair in response to IR. One original scientific publication. |
URL | https://pubmed.ncbi.nlm.nih.gov/31613024/ |
Title | BrdU assay for ssDNA |
Description | We have improved a method for better visualisation of single stranded DNA fragments in human cells. |
Type Of Material | Technology assay or reagent |
Year Produced | 2021 |
Provided To Others? | Yes |
Impact | - one original research publication - this protocol has also been published |
URL | https://pubmed.ncbi.nlm.nih.gov/34888531/ |
Title | SPRTN haploinsufficient HeLa Cell Line |
Description | We have created a SPRTN haploinsufficient cell line and published this tool in Vaz et al. Mol Cell 2016 |
Type Of Material | Cell line |
Year Produced | 2016 |
Provided To Others? | Yes |
Impact | We have directly demonstrated that inactivation of SPRTN causes formation of endogenous DNA-protein cross links. Other researchers requested this cell line for their work and we provided it by the MTA. |
URL | https://pubmed.ncbi.nlm.nih.gov/27871366/ |
Title | TEX264 knock out HeLa and HEK293 cell lines |
Description | We have created TEX264 knock out HeLa and HEK293 for our research. |
Type Of Material | Cell line |
Year Produced | 2020 |
Provided To Others? | Yes |
Impact | Using this tool we have directly validated our findings in human cells and demonstrated that TEX264 is essential for Top1-cc repair. One original scientific publication. A new project has been awarded based on this discovery. |
URL | https://pubmed.ncbi.nlm.nih.gov/32152270/ |
Description | Dr Philipp; The role of SPRTN-CHK1 loop in Zebrafish |
Organisation | University Hospital Tuebingen |
Country | Germany |
Sector | Academic/University |
PI Contribution | -intellectual input on the SPRTN-CHK1 loop |
Collaborator Contribution | -performed Zebrafish experiments |
Impact | -Halder et al., manuscript submitted and preprint published in Bioarchive, 2018 |
Start Year | 2017 |
Description | Dr Raimundo Freire; ATX3 in DNA replication and repair |
Organisation | University of La Laguna |
Country | Spain |
Sector | Academic/University |
PI Contribution | We have discovered the deubiquitinase Ataxin 3 (ATX3) in DNA damage response and DNA replication. We are analysing p97 proteome after ionizing radiation. |
Collaborator Contribution | My partner developed ATX3 specific antibodies and purified ATX3 protein. My partner is performing bioinformatic analysis of p97 proteome after ionizing radiation. |
Impact | -Abhay et al., EMBO J, 2019. |
Start Year | 2017 |
Description | Evotec SE company/ Identification of a first-class inhibitor of SPRTN protease |
Organisation | Evotec |
Country | Germany |
Sector | Private |
PI Contribution | We have identified SPRTN protease as a new druggable target for cancer therapy. |
Collaborator Contribution | In collaboration with Evotec SE we had prepared a grant proposal for the identification and design of SPRTN protease inhibitors and we have beem awarded with £500K by the Lab282. Now, Evotec SE is screening for the hits. |
Impact | This is a multidisciplinary collaboration between Industry that provides the expertise in chemistry and drug discovery and my lab that provides the expertise in biology and medicine. We are currently in the last stage to establish a spin-off from this collaboration. |
Start Year | 2017 |
Description | Jiri Bartek, PARP1-p97 study |
Organisation | Danish Cancer Society |
Department | Danish Cancer Society Research Center |
Country | Denmark |
Sector | Academic/University |
PI Contribution | We have found that the p97 ATPase removes cytotoxic trapped PARP1 from chromatin. |
Collaborator Contribution | -provided p97 inhibitor CuET |
Impact | -original scientific publication |
Start Year | 2019 |
Description | Prof Benedikt Kessler, Identification of the p97 proteome by quantitative mass-spectrometry. |
Organisation | University of Oxford |
Department | Nuffield Department of Medicine |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We are analysing the p97 proteome by quantitative mass-spectrometry after ionising radiation in different cellular fractions. Prof Kessler provides mass-spectrometry expertise for this project. |
Collaborator Contribution | Prof Kessler provides expertise and technology on quantitative mass-spectrometry analysis. This is one of the essential collaborations I have, as many completed and ongoing projects in the lab are based on this collaboration. |
Impact | 1. John Fielden, Katherine Wiseman, Ignacio Torrecilla, Shudong Li, Samuel Hume, Shih-Chieh Chiang, Annamaria Ruggiano, Abhay Narayan Singh, Raimundo Freire, Sylvana Hassanieh, Enric Domingo, Iolanda Vendrell, Roman Fischer, Benedikt M. Kessler, Timothy S. Maughan, Sherif F. El-Khamisy, Kristijan Ramadan*. TEX264 coordinates p97- and SPRTN-mediated resolution of topoisomerase 1-DNA adducts. Nature Communications, 2020. in press. DOI: 10.1038/s41467-020-15000-w |
Start Year | 2017 |
Description | Prof Christopher J Lord |
Organisation | Institute of Cancer Research UK |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We performed biochemical analysis of PARP1 interaction with the p97 system. |
Collaborator Contribution | My collaborator identify the interaction between PARP1 and the p97 system by mass-spectrometry analysis. |
Impact | -grant award -original scientific publication -connection with other research groups (networking) Yes, this collaboration is multi-disciplinary, between clinical oncologists, clinical scientists and basic scientists. |
Start Year | 2019 |
Description | Prof Dianov; Interplay between base excision repair protein XRCC1 and ALDH2 predicts overall survival in lung and liver cancer patients. |
Organisation | Russian Academy of Sciences |
Department | Institute of Cytology and Genetics, Siberian Branch of the Russian Academy of Sciences |
Country | Russian Federation |
Sector | Public |
PI Contribution | My team and I have analysed DNA-protein crosslinks in cells where XRCC1 and ALDH2 were inactivated. We are currently working on the regulation of G1/S checkpoint by the NUCKS1-SKP2 axis. |
Collaborator Contribution | My partner (Prof Dianov) found that low ALDH2 expression levels associated with high XRCC1 expression levels are indicative for a poor overall survival, particularly in lung and liver cancer patients. In addition, they found that Mithramycin A, a XRCC1 expression inhibitor, efficiently kills cancer cells expressing low levels of ALDH2. My partner (Prof Dianov) has identified the NUCKS1-SKP2 axis. |
Impact | Interplay between base excision repair protein XRCC1 and ALDH2 predicts overall survival in lung and liver cancer patients. Chen X, Legrand AJ, Cunniffe S, Hume S, Poletto M, Vaz B, Ramadan K, Yao D, Dianov GL. Cell Oncol (Dordr). 2018 Oct;41(5):527-539. doi: 10.1007/s13402-018-0390-8. Epub 2018 Aug 7. PMID: 30088263 A unified model for the G1/S cell cycle transition. Hume S, Dianov GL, Ramadan K. Nucleic Acids Res. 2020 Dec 16;48(22):12483-12501. doi: 10.1093/nar/gkaa1002. |
Start Year | 2017 |
Description | Prof Dianov; NUCKS1-SKP2-p21/p27 axis controls S phase |
Organisation | University of Oxford |
Department | Department of Oncology |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We have delineated the molecular mechanism of NUCKS1-SKP2-p21/p27 axis and how it controls S phase entry. |
Collaborator Contribution | Prof Dianov initiated this project and helped us to recruit one fully-funded DPhil student. |
Impact | -original scientific publication -review paper -one DPhil student |
Start Year | 2017 |
Description | Prof Geoff S Higgins, TOPK sensitises tumour cells |
Organisation | University of Oxford |
Department | Department of Oncology |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We analysed how TOPK regulates DNA replication. |
Collaborator Contribution | Prof Higgins groups found that targeting TOPK sensitises tumour cells to radiation-induced damage. |
Impact | -two original scientific publications |
Start Year | 2019 |
Description | Prof Kiltie; MRN disassembly |
Organisation | University of Oxford |
Department | Department of Oncology |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We have discovered how MRN complex, the main DNA double strand break nuclease, is disassembled and regulated at sites of DNA double strand break after ionising radiation. |
Collaborator Contribution | Prof Kiltie validated this model in a mouse system and patient materials. |
Impact | -original scientific publication -review publication -one shared DPhil student This is multidisciplinary collaboration between clinical oncologists and basic scientists. |
Start Year | 2017 |
Description | Prof Sherif El-Khamisy |
Organisation | University of Sheffield |
Department | Department of Molecular Biology and Biotechnology |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We have initiated and led the research project and as the results of it, we have identified a novel pathway for the resolution of toxic Top1-cleavage complexes. Our finding could be potentially translated for cancer therapy. |
Collaborator Contribution | Prof El-Khamisy provided his expertise on Top1-cleavage complex proteolysis in vitro and perform one of the important experiments for our manuscript. |
Impact | John Fielden, Katherine Wiseman, Ignacio Torrecilla, Shudong Li, Samuel Hume, Shih-Chieh Chiang, Annamaria Ruggiano, Abhay Narayan Singh, Raimundo Freire, Sylvana Hassanieh, Enric Domingo, Iolanda Vendrell, Roman Fischer, Benedikt M. Kessler, Timothy S. Maughan, Sherif F. El-Khamisy, Kristijan Ramadan*. Nature Communications, 2020, in press, 10.1038/s41467-020-15000-w |
Start Year | 2017 |
Description | Prof Timothy Maughan, The role of DPC proteolysis in colorectal cancer patient response |
Organisation | University of Oxford |
Department | Department of Oncology |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | We have identified that the SPRTN protease plays an essential role to colorectal cancer patient response to Irinotecan-based chemotherapy. |
Collaborator Contribution | Prof T. Maughan provided access to clinical samples of colorectal cancers and is actively involved in clinical expertise on this project. |
Impact | John Fielden, Katherine Wiseman, Ignacio Torrecilla, Shudong Li, Samuel Hume, Shih-Chieh Chiang, Annamaria Ruggiano, Abhay Narayan Singh, Raimundo Freire, Sylvana Hassanieh, Enric Domingo, Iolanda Vendrell, Roman Fischer, Benedikt M. Kessler, Timothy S. Maughan, Sherif F. El-Khamisy, Kristijan Ramadan*. Nature Communications, 2020, in press, 10.1038/s41467-020-15000-w |
Start Year | 2018 |
Description | Prof Yosef Shiloh: Investigation of the novel E3 ubiquitin ligases in DNA damage response , Tel Aviv University, Israel |
Organisation | Tel Aviv University |
Country | Israel |
Sector | Academic/University |
PI Contribution | We have characterised the recruitment of two new E3-ubiquitin ligases to sites of DNA lesions. |
Collaborator Contribution | The group of Prof Shiloh has identified two new ubiquitin ligases by high-throughput screen. |
Impact | We have discovered the importance of fine-tuning of the ubiquitin signal at sites of DNA double strand breaks for cell survival. This discovery resulted in one original scientific publication in a prestigious scientific journal, Molecular Cell. |
Start Year | 2017 |
Description | Prof. Vallis; A three-in-one-bullet for oesophageal cancer: replication fork collapse, spindle attachment failure and enhanced radiosensitivity generated by a ruthenium(ii) metallo-intercalator. |
Organisation | University of Oxford |
Department | Department of Oncology |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | My team and I have analysed DNA replication fork progression and elucidated one of the mechanisms how a ruthenium(ii) metallo-intercalator causes DNA replication stress and cell killing. |
Collaborator Contribution | My partners identified ruthenium(ii) metallo-intercalator as a potential chemical that kills human cells. |
Impact | DOI: 10.1039/C7SC03712K |
Start Year | 2017 |
Description | Yves Pommier_p97-PARP1 study |
Organisation | National Institutes of Health (NIH) |
Department | National Cancer Institute (NCI) |
Country | United States |
Sector | Public |
PI Contribution | Elucidating the physical and functional interactions between PARP1 and p97. |
Collaborator Contribution | Prof Pommier's team reconstituted one part of our findings in vitro. |
Impact | -original scientific publication |
Start Year | 2021 |
Title | Biomarker for stratification of patients with colorectal cancer |
Description | The present invention relates to a method for identifying a cancer that is predicted to respond to a chemotherapeutic agent. In particular, to a method for identifying a cancer that is predicted to respond to treatment with a topoisomerase 1 (TOP1) inhibitor, such as irinotecan. The cancer may be metastatic colorectal cancer. The invention also extends to a method of treating cancer in a subject and a method of selecting a cancer patient for treatment with a cancer therapy. The invention further extends to use of cancer cells, such as primary colon cancer cells, as a biomarker for a patient's response to treatment (insensitivity or sensitivity) with a particular chemotherapeutic agent, such as a TOP1 inhibitor. |
IP Reference | JA100287P.GBA |
Protection | Patent application published |
Year Protection Granted | 2019 |
Licensed | No |
Impact | Based on this patent I am currently negotiating with the investors to start with a spin-off company. |
Description | AMAC-COVID19 talk |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | This was a public talk on COVID-19 origin, pathology and vaccine for the Association of Alumni and Friends of Croatian Universities in the United Kingdom (AMAC/Almae Matris Alumni Croaticae UK). |
Year(s) Of Engagement Activity | 2021 |
URL | https://amacuk.net/godisnja-predavanja |
Description | Ignacio Torrecilla, CRUK fundraiser visit |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Supporters |
Results and Impact | Guiding a group of people around our labs and discussing our research projects with them. |
Year(s) Of Engagement Activity | 2016 |
Description | Ignacio Torrecilla, Oxfordshire Science Festival, 25-26 June 2016. Stall at Broad Street, Oxford. |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | Ignacio discussed with a broad public what makes a drug good for cancer treatment. |
Year(s) Of Engagement Activity | 2016 |
Description | Interview for Croatian national news |
Form Of Engagement Activity | A magazine, newsletter or online publication |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | I am regularly giving the interviews about science and my research to various Croatian National Newspapers. |
Year(s) Of Engagement Activity | 2017,2018,2019 |
URL | https://www.vecernji.hr/techsci/gen-starenja-i-raka-jetre-kljuc-za-boj-s-tumorima-965297 |
Description | John Fielden, public talk to high school pupils, Department of Oncology, Oxford, June 2016 |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Schools |
Results and Impact | John explained scientific concepts to high school students. The purpose was to make the University of Oxford an appealing place to apply to for their undergraduate degrees. |
Year(s) Of Engagement Activity | 2016 |
Description | MRC Festival 2019, Bletchley, Tesco Extra (Dr Abhay Narayan Singh) |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | We interacted with more than 100 people visiting the venue (Tesco Extra, Bletchley) to make them aware about ongoing cancer research in the UK and future possibilities. We discussed about our research and clinical trials. We especially focused on how hospitals are adopting the research outcomes for treatment of cancer and in benefit of patients. We also advertised and discussed that our research is funded by MRC, and that the MRC funding comes from their taxes. |
Year(s) Of Engagement Activity | 2019 |
Description | Public Engagement at UKRI Coffee Morning Webinar |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | To promote my MRC supported research work on Cancer among general public and Macmillan Cancer Support Charity. |
Year(s) Of Engagement Activity | 2020 |
Description | SPRTN-CHK1 axis, Departmental Press Release 2019 |
Form Of Engagement Activity | Engagement focused website, blog or social media channel |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | Press release from Department of Oncology on our discovery of an evolutionary conserved SPRTN-CHK1 loop for genome stability. |
Year(s) Of Engagement Activity | 2019 |
Description | STEM for Britain 2022 |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Policymakers/politicians |
Results and Impact | "STEM for BRITAIN is a major scientific poster competition and exhibition which has been held in Parliament since 1997, and is organised by the Parliamentary & Scientific Committee. Chaired by Stephen Metcalfe MP, its aim is to give members of both Houses of Parliament an insight into the outstanding research work being undertaken in UK universities by early-career researchers". |
Year(s) Of Engagement Activity | 2022 |
URL | https://stemforbritain.org.uk/ |
Description | Samuel Hume, Department of Oncology, University of Oxford, October 2017, talk to CRUK donators |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Supporters |
Results and Impact | Samuel discussed his DPhil project and research in our group to CRUK donators. |
Year(s) Of Engagement Activity | 2017 |
Description | Samuel Hume, talk to high-school pupils |
Form Of Engagement Activity | Participation in an open day or visit at my research institution |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Schools |
Results and Impact | Samuel Explained concepts of research in Oncology to high-school students. |
Year(s) Of Engagement Activity | 2016 |
Description | TEX264, University Oxford Blog 2020 |
Form Of Engagement Activity | Engagement focused website, blog or social media channel |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | Oxford University Blog releases news on our discovery of TEX264 and its potential importance for cancer therapy. This press release was based on our publication in Nature Communications. |
Year(s) Of Engagement Activity | 2020 |
URL | http://www.ox.ac.uk/news/science-blog |
Description | TV interviews |
Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Other audiences |
Results and Impact | More than 10 TV interviews April 2020 until now on COVID-19 pandemic and vaccine development for Croatian National TV and regional TV (Al Jazeera Balkan). |
Year(s) Of Engagement Activity | 2020,2021 |
URL | https://www.youtube.com/watch?v=o1nS_JLgJsU |