Catchment-scale processes contributing to transmission of antibiotic resistance in bathing waters
Lead Research Organisation:
University of Exeter
Department Name: Institute of Biomed & Clinical Science
Abstract
The ability of disease-causing bacteria to survive and grow in the presence of antibiotics designed to kill them (antibiotic resistance) has been described as one of the greatest threats to humans. This threat not only poses a risk to human health, but also to animal and plant health. It will have significant impacts on the economy through increased cost of treatment, longer hospital stays, and higher rates of morbidity and mortality. Increasing antibiotic resistance will also result in loss of economic productivity. In the World Bank's 2017 report on the devastating effect of drug-resistant infections on the global economy, they recommended that the key to tackling resistant infections should be to take action and make investments in the following sectors: Health, Agriculture, Urban Development, and Water and Sanitation. In this fellowship, I will conduct research, in collaboration with a representative of the Water and Sanitation Industry (South West Water), that will be relevant for many of these sectors. I aim to use cutting-edge technology to estimate the abundance of antibiotic resistant bacteria in bathing waters, which are impacted by various pollution sources (including those from wastewater treatment plants and agriculture). I will also investigate catchment-level processes which contribute to the transmission of antibiotic resistant bacteria to humans in these key environments. Bathing waters represent aquatic environments which many members of the public come into close contact with, leading to potential exposure to high levels of resistant bacteria. My previous research suggests that there are millions of human exposure events to one type of clinically important resistant Escherichia coli per year in UK bathing waters. The proposed research will focus on all resistance mechanisms in a key indicator bacterium, E. coli, which is quantified at all designated bathing beaches in the UK, allowing quantitative risk assessment of human exposure to be undertaken. By understanding the reasons for increased resistance and therefore increasing exposure risk, we can inform mitigation strategies for reducing microbial pollution.
The results of this research have global significance in terms of identifying and understanding the sources and transmission of antibiotic resistance. Not only will the findings be of academic interest but the information generated by the proposed research can help inform discussions about land and water management at both regional and national levels. In addition, the information generated by this research will contribute valuable knowledge to inform the water industry's response to the emerging problem of environmental antimicrobial resistance. Enhanced treatment methods are extremely costly and energy intensive, with consequences for society through higher water bills and greater greenhouse gas emissions. There is much public concern about these matters. Without sufficient evidence of the water industry's contribution to the problems and possible solutions, or of the degree of risk posed to human health, there is little incentive to devise and invest in innovative solutions to reduce the dissemination of antibiotic resistant bacteria to receiving waters.
The results of this research have global significance in terms of identifying and understanding the sources and transmission of antibiotic resistance. Not only will the findings be of academic interest but the information generated by the proposed research can help inform discussions about land and water management at both regional and national levels. In addition, the information generated by this research will contribute valuable knowledge to inform the water industry's response to the emerging problem of environmental antimicrobial resistance. Enhanced treatment methods are extremely costly and energy intensive, with consequences for society through higher water bills and greater greenhouse gas emissions. There is much public concern about these matters. Without sufficient evidence of the water industry's contribution to the problems and possible solutions, or of the degree of risk posed to human health, there is little incentive to devise and invest in innovative solutions to reduce the dissemination of antibiotic resistant bacteria to receiving waters.
Publications
Leonard AF
(2022)
Natural recreational waters and the risk that exposure to antibiotic resistant bacteria poses to human health.
in Current opinion in microbiology
Stanton I
(2022)
Existing evidence on antibiotic resistance exposure and transmission to humans from the environment: a systematic map
in Environmental Evidence
Stanton IC
(2020)
What is the research evidence for antibiotic resistance exposure and transmission to humans from the environment? A systematic map protocol.
in Environmental evidence
Description | The award has generated data on the abundance and diversity of antibiotic resistance genes harboured by the faecal indicator bacterium, Escherichia coli, which were isolated from popular coastal bathing waters in England over two years. This includes data on how these abundances change over time at each of the study sampling locations. Another objective was to identify catchment-scale processes that contribute antibiotic-resistance gene bearing E. coli to bathing waters where people are exposed. A comprehensive geographically-linked dataset encompassing various catchment-scale processes across humans, animals and the environment, was successfully compiled from publicly available data for processes thought to be important in influencing the abundance and diversity of antibiotic resistant E. coli. Robust methods were used to investigate associations between these catchment processes and the abundance of resistance genes harboured by E. coli for individual catchments and for all catchments. A key finding from this analysis was that catchment-scale processes are highly variable between catchments, and this precludes identifying universal processes governing the spread of antibiotic resistant bacteria in coastal waters in general. Another finding was that E. coli is too homogenous a marker for antimicrobial resistance to detect catchment-scale processes at the resolution considered in this study. Therefore, this award has contributed to our understanding of the variation in abundance and diversity of antibiotic resistance genes harboured by bacteria that are capable of human colonisation and infection in coastal bathing waters. The findings are informing the design of follow-up studies aiming to identify and characterise catchment-scale processes influencing antimicrobial resistance dissemination in the environment. |
Exploitation Route | The outcomes should inform the design of future studies looking to understand and quantify the sources of antibiotic resistant bacteria in the environment, particularly surface waters. |
Sectors | Agriculture, Food and Drink,Environment,Healthcare,Leisure Activities, including Sports, Recreation and Tourism |
Description | Continuing professional development |
Geographic Reach | Multiple continents/international |
Policy Influence Type | Influenced training of practitioners or researchers |
Description | Canada_IPAP - Anglo-Canadian Collaboration on Antimicrobial resistance |
Amount | £189,647 (GBP) |
Funding ID | BB/X012840/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 06/2023 |
End | 05/2025 |
Description | Developing a conceptual framework to improve understanding of AMR in livestock systems: translating research into policy and practice |
Amount | £987,216 (GBP) |
Funding ID | BB/T004452/1 |
Organisation | Biotechnology and Biological Sciences Research Council (BBSRC) |
Sector | Public |
Country | United Kingdom |
Start | 07/2019 |
End | 05/2022 |
Description | Environmental Evidence for the Future 2018 |
Amount | £99,929 (GBP) |
Funding ID | NE/S015965/1 |
Organisation | Natural Environment Research Council |
Sector | Public |
Country | United Kingdom |
Start | 01/2019 |
End | 09/2019 |
Description | GW4+ DTP: |
Amount | £97,000 (GBP) |
Organisation | Natural Environment Research Council |
Sector | Public |
Country | United Kingdom |
Start | 09/2022 |
End | 02/2026 |
Description | Horizon Europe: Reducing climate based health risks in blue environments: Adapting to the climate change impacts on coastal pathogens |
Amount | € 9,815,814 (EUR) |
Funding ID | 101057764 |
Organisation | European Commission |
Sector | Public |
Country | European Union (EU) |
Start | 09/2022 |
End | 08/2025 |
Title | Catchment-scale processes |
Description | Collection of time- and/or space- matched catchment-scale processes occurring during sampling. Includes weather data (e.g. rain, temperature), antibiotic prescribing in community settings, land cover types, crop types, location and size of wastewater treatment plants, time and locations of combined sewer overflows in the 2018 and 2019 bathing seasons. (May 2018- September 2018, May 2019 - September 2019) |
Type Of Material | Database/Collection of data |
Year Produced | 2021 |
Provided To Others? | No |
Impact | Methods and outcomes are being shared with researcher in Environment Agency employed on PATH-SAFE. |
Title | Phenotypic and genotypic characteristics of cefotaxime-resistant bacteria isolated from coastal waters and water users guts |
Description | DNA from cefotaxime-resistant bacteria isolated from coastal waters and water users' guts has been sequenced to identify presence and type of virulence, and resistance genes, as well as insertion sequences in resistant isolates. Dataset also includes results of antimicrobial susceptibility testing to a panel of 12 antibiotics using the disk diffusion method. |
Type Of Material | Database/Collection of data |
Year Produced | 2019 |
Provided To Others? | No |
Impact | The dataset has been used for the basis of generating hypotheses among students, colleagues and potential collaborators. It has also allowed for the selection of strains that will undergo whole genome sequencing to investigate genetic context of various resistance genes, and associations with virulence |
Title | Resistance genes present in E. coli in bathing waters |
Description | The relative abundance and diversity of antibiotic resistance genes in E. coli isolated from bathing waters . |
Type Of Material | Database/Collection of data |
Year Produced | 2021 |
Provided To Others? | No |
Impact | This dataset is being shared with researcher in Environment Agency employed on PATH-SAFE (multimillion-pound cross-government programme to track foodborne pathogens and antimicrobial resistance). |
Title | Sequence data from coastal bathing water Escherichia coli in 10 English bathing water catchments |
Description | Contains data on the samples from 10 well-characterised bathing water catchments, which have been studied. Includes Escherichia coli density data, weather variables, data derived from analysis of E. coli metagenome sequencing. |
Type Of Material | Database/Collection of data |
Year Produced | 2020 |
Provided To Others? | No |
Impact | Dataset is also being used to facilitate a masters by research student based at the Environment Agency. This research project is also contributing valuable phenotypic resistance data. |
Title | Water metagenomes |
Description | Sequence files derived from next generation sequencing of DNA extracted from surface water samples within study catchments |
Type Of Material | Database/Collection of data |
Year Produced | 2021 |
Provided To Others? | No |
Impact | This collection of data is forming the basis of a grant proposal, which is being supported by the Environment Agency |
Description | AMR showcase at Westminster |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Policymakers/politicians |
Results and Impact | I was invited to present my research at the UK Parliament AMR reception event, held on 25th February 2020. 40 researchers leading in AMR research from 10 UK-based research organisations were invited to present their work at an event, which was open to the public. Members of parliament and Prof Chris Whitty (Chief Medical Officer) attended. |
Year(s) Of Engagement Activity | 2020 |
URL | https://www.linkedin.com/feed/update/urn:li:activity:6638488771438477312/ |
Description | Beachwise Forum Meeting |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Industry/Business |
Results and Impact | I was invited by Southwest Water (project partner and business) to give an oral presentation on the research that I am currently doing on catchment-level processes contributing to the transmission of antibiotic-resistant bacteria in coastal bathing waters. The meeting was attended by Southwest Water staff, as well as government departments e.g the Environment Agency (also project partners) and Marine Management Organisation, and Surfers Against Sewage (Supporters), and beach managers. The presentations at the meeting were filmed by Ofwat for dissemination on their website. |
Year(s) Of Engagement Activity | 2018 |
URL | https://www.southwestwater.co.uk/environment/rivers-and-bathing-waters/beachwise/ |
Description | Carrick U3A Science Group |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | I was invited to give a talk about my research to a local audience comprised of 40 members of the general public who regularly meet to discuss scientific research and listen to presentations from scientists in the region. I presented the work that I am currently undertaking for my NERC-funded industrial innovation fellowship, and the risk that antibiotic-resistant bacteria pose to human health. The talk was followed by a question and answer session, which allowed for an engaged debate with everyone who attended. I received feedback from the meeting organiser after the event that the talk was enjoyed by the attendees: they found the topic very interesting and important and it generated a good deal of discussion over the lunch break. I provided my powerpoint slides and additional resources for the organiser to put on their website for the interest of members who were not able to attend the session. |
Year(s) Of Engagement Activity | 2018 |
Description | Conference |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Other audiences |
Results and Impact | I presented my work on catchment scale processes contributing to antibiotic resistant bacteria in coastal bathing waters at the international conference, environmental dimensions of antibiotic resistance in September 2022. It prompted questions and discussions afterwards, along with audience members reaching out to me about the findings and ongoing work. |
Year(s) Of Engagement Activity | 2022 |
Description | Continuing professional development courses |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | I designed and delivered a remote continuing professional development course on antimicrobial resistance, giving lectures about my previous and active research to understand how antibiotic resistant bacteria enter natural environments, and what it means for human health once they are there. I also invited industry contacts as well as research students to deliver lectures about the work I supervise, and their work in the Environment Agency on surveillance on environmental antimicrobial resistance. The lectures initiated thoughtful questions and discussions, and participants reported finding the course engaging, informative, and multi-perspective. |
Year(s) Of Engagement Activity | 2023 |
Description | Dispatches interview |
Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Media (as a channel to the public) |
Results and Impact | Interview for Channel 4 Dispatches about sewage pollution, and the contribution this makes to AMR in bathing waters and impacts on human health |
Year(s) Of Engagement Activity | 2023 |
Description | Interview for international news |
Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Media (as a channel to the public) |
Results and Impact | Interview for Euronews (broadcast in 160 countries) on antimicrobial resistance in the environment, including giving evidence about health impacts and sources. |
Year(s) Of Engagement Activity | 2023 |
Description | Interview national media |
Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Media (as a channel to the public) |
Results and Impact | Gave interviews about impacts of sewage pollution on human health, and the risk that antibiotic resistant bacteria poses to bathers |
Year(s) Of Engagement Activity | 2022 |
Description | JPIAMR towards developing an environmental surveillance strategy for antimicrobial resistance |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | I was invited to give a presentation at the first meeting of the JPIAMR-funded working group "Towards Developing an International Environmental AMR Surveillance Strategy". I gave a presentation on the work that I have been conducting during the course of my independent fellowship into catchment-scale processes contributing to antibiotic resistance in coastal bathing waters. The techniques that I am developing in my research provide new ways to monitor the abundance and diversity of antibiotic-resistant bacteria in coastal bathing waters, and offer insights into the risks to bathers of exposure to antibiotic-resistant bacteria, and points for actions which may reduce levels of antibiotic-resistant bacteria. The audience was comprised primarily of professionals from various departments in DEFRA, such as the Veterinary Medicines Directorate, Animal and Plant Health Agency, Environment Agency, Public Health England, and Cefas, as well as a number of international researchers (see URL). The presentations sparked interesting questions about what is possible and what is practical in terms of planning and delivering an international environmental AMR surveillance strategy. Planned outputs include a paper outlining a suggested roadmap towards delivering a practical and comprehensive surveillance strategy for AMR in the environment aimed at policymakers. |
Year(s) Of Engagement Activity | 2019 |
URL | https://www.jpiamr.eu/wp-content/uploads/2018/11/Summary_Towards-Developing.pdf |
Description | Lecture at Summer School on Oceans and Human Health |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Postgraduate students |
Results and Impact | I attended a summer school on oceans and human health, presenting to a varied group of approximately 60 participants on past and current research into antimicrobial resistance in the environment. The intended purpose of the summer school was to take an interdisciplinary perspective on oceans and human health, and to develop an agenda for this topic in Europe. People attending included postgraduate students, but also representatives from the European Environment Agency, sustainable ecotourism businesses, and other academics. |
Year(s) Of Engagement Activity | 2019 |
URL | https://www.azti.es/en/eventos/azti-sophie-project-summer-school-2019-does-human-health-and-wellbein... |
Description | Presentation at international meeting on foodborne and environmental AMR |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Professional Practitioners |
Results and Impact | i attended a workshop in Niagara-on-the-lake, Canada, and presented my past and current research on AMR in bathing waters. Attendees are currently working towards writing white papers on the themes discussed at the workshop for publication. |
Year(s) Of Engagement Activity | 2019 |
Description | Scientists at Sea podcast |
Form Of Engagement Activity | A broadcast e.g. TV/radio/film/podcast (other than news/press) |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Undergraduate students |
Results and Impact | I gave an interview with Ethan and Molly at the University of Exeter for their podcast, Scientists at Sea. I talked about the project that I am currently undertaking (catchment-scale processes contributing to transmission of antibiotic-resistant bacteria in coastal waters), as well as my past research underpinning this work and answered questions about antibiotic-resistant bacteria in natural environments. The episode has been listened to 74 times by people from the UK (40 people), and 12 other countries around the world (United States, France, Australia, Germany, Ireland, Algeria, Argentina, Malaysia, Japan, United Arab Emirates, Malta and Sweden). The episode was shared on social media (Facebook, and Twitter) by the University of Exeter, University of Roehampton, journalists and environmental charities. |
Year(s) Of Engagement Activity | 2018 |
URL | https://blogs.exeter.ac.uk/exetermarine/2018/08/29/scientists-at-sea-podcast-whats-in-the-water-with... |