Building a Better Egg Timer?
Lead Research Organisation:
University of Oxford
Department Name: Academic Services & Uni Collections
Abstract
Fossil and DNA evidence suggests that our species, Homo sapiens, emerged nearly 200,000 years ago in Africa, but until recently it seemed that many of the signs of what we would consider 'modern behaviour' were not evident until after 50,000 years ago. The archaeological evidence for this crucial period is exceedingly sparse and poorly dated. The dramatic climate and environmental changes that occurred during this period were likely to be critical influences, but in the absence of reliable dating methods it is difficult to link the patchy human record with existing continuous global climate records, or regional records where they exist. The picture has begun to change with the development of new dating methods and accumulating evidence for the early occurrence of modern behaviours, such as marine resource exploitation and the appearance of decorated or decorative objects in several sites. The latter include indications that ostrich eggs were used as containers and as decorative beads. However, at present none of these artefacts can be dated directly and precisely. One method that can be used to date shells and eggshells directly is amino acid racemization (AAR) geochronology, which measures the decay of proteins. Because the decay rate is driven by time and temperature, the relative age can be deduced by comparison with other samples. Determination of an absolute date is more challenging. In order to provide a robust and precise age estimate, the mechanisms that drive the decay reaction must be understood. We have been developing a refinement of amino acid geochronology, called 'Intra-crystalline Protein Decomposition' (IcPD), isolating and analysing only the protein from a particular fraction of shells, where the proteins have been trapped within the shell crystals. This effectively forms a protein 'time capsule', within which changing concentrations of the products and reactants of protein decay should enable us to pick apart the reaction pathways. In the past we have been limited by crude measurements of decay, but technological advances, notably in mass spectrometry, now enable us to observe both products and reactants in considerable detail. We plan to explore the reaction pathways in ostrich eggshell fragments, the ultimate 'egg-timer'. Preliminary results reveal the first evidence of a decay pathway in the main protein entrapped in ancient shell, called struthiocalcin. The protein first appears to split into three pieces and each piece is 'nibbled away' from one end. By using a combination of model systems (synthetic peptides, heated shells and shell proteins) and shell fragments recovered from the stratified and well-dated archaeological sequence in Elands Bay Cave, South Africa, we can explore how this initial pattern develops. Once a more complete picture of decay is understood, we will put together a model of the decay path (which identifies the temperature sensitivity of key reactions). We will then apply this model to estimate the age of eggshell fragments found in a rich archaeological sequence at Pinnacle Point, South Africa, to refine the chronological framework of these early modern human sites. But the ostrich eggshells are more than just egg-timers - if we look at the isotopic composition of the eggshells, this also reveals information about climate and environment. Thus we can establish a robust, directly dated record of changes in vegetation and importantly, in aridity, for the region. This data can be linked to longer climate records to build up a more complete picture of the relationships between changing human behaviour and changing environments.
People |
ORCID iD |
Julia Lee-Thorp (Principal Investigator) |
Publications
Lee-Thorp J
(2015)
Holocene Environmental Change at Wonderwerk Cave, South Africa: Insights from Stable Light Isotopes in Ostrich Eggshell
in African Archaeological Review
Description | The protein in ostrich eggshell, struthiocalcin, is extremely robust and as long as the shell has not been burnt, the suite of amino acids captured within the shell crystals provide a good indication of age, via their differing decay processes. At the same time, the stable light isotopes of carbon and oxygen provide robust indications of the vegetation being eaten by the ostrich (and hence vegetation cover) and local moisture conditions. Egshell reflects conditions during the breeding season, so the vegetation and climate signals are also highly variable as they reflect intra-annual conditions. A further spin-out of this research is the finding (in new research by partners) that the reason struthiocalcin is so long-lived is because of its binding to the mineral calcite. Its presence has been detected even in eggshell over 3 millions years old. |
Exploitation Route | Our findings are indicating that we have an important new dating mechanism which will apply as a complement to OSL dating, and in the important period of emergence of modern humans. At the same time it is an indication of prevailing conditions. The complementarity with OSL is important because there have been some controversies generated by that technique but our method offers an indeependent means entirely. |
Sectors | Environment,Culture, Heritage, Museums and Collections |
Description | We designed and delivered a workshop on amino acid racemisation and its application as a dating tool |
First Year Of Impact | 2012 |
Sector | Education |
Impact Types | Cultural,Societal |
Description | Collins EP/I001514/1 Exceptional preservation of eggshell peptides |
Organisation | University of York |
Department | BioArCh |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Spin on research funded by Engineering and Physical Sciences Research Council grant number EP/I001514/1 |
Collaborator Contribution | Material research on same materials (fossil eggshell) |
Impact | Article in preparation |
Start Year | 2015 |
Description | Paleoscape: NSF, IPG - Developing and Testing an Integrated Paleoscape Model for the early Middle and Late Pleistocene of the South Coast of South Africa ( led by C. Marean (ASU) $1,000,000), participating scientist |
Organisation | Arizona State University |
Country | United States |
Sector | Academic/University |
PI Contribution | Participating scientist in NSF, IPG - Developing and Testing an Integrated Paleoscape Model for the early Middle and Late Pleistocene of the South Coast of South Africa (PI C. Marean (ASU) $1,000,000), |
Collaborator Contribution | PI |
Impact | Contribution of Curtis Marean to Building a better Eggtimer project (samples, collaboration, workshop, articles in prep) |
Start Year | 2011 |
Description | Outreach for Iziko museum, South Africa |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | Developed materials for the Iziko museum in Cape Town, South Africa, designed to be used for museum visits by children and adults. Development of collaboration with museums in South Africa. |
Year(s) Of Engagement Activity | 2013,2014 |