Reversing senescence in 3D Tissue models of in vitro ageing
Lead Research Organisation:
Queen Mary University of London
Department Name: Blizard Institute of Cell and Molecular
Abstract
Senescent cells accumulate as we age, and are linked to the loss of regenerative potential and diminished function in ageing tissues. Senescence is classically viewed as an irreversible, stable arrest, however, we have recently developed the ability to reverse this process. This rejuvenation protocol provides a unique opportunity to unravel the molecular mechanisms that drive senescence and very possibly human ageing.
This proposal aims to explore the modulation of senescence in skin model systems, dissecting the interplay between keratinocytes and fibroblasts, and the relative contributions of senescent fibroblasts to the skin ageing phenotypes. We will be particularly interested to ask if the rejuvenation protocol can alleviate ageing phenotypes. We will use high-throughput siRNA screening and sophisticated 3D systems to model complex in vivo relationships and examine the mechanisms of skin ageing.
This proposal aims to explore the modulation of senescence in skin model systems, dissecting the interplay between keratinocytes and fibroblasts, and the relative contributions of senescent fibroblasts to the skin ageing phenotypes. We will be particularly interested to ask if the rejuvenation protocol can alleviate ageing phenotypes. We will use high-throughput siRNA screening and sophisticated 3D systems to model complex in vivo relationships and examine the mechanisms of skin ageing.
People |
ORCID iD |
| Cleo Bishop (Primary Supervisor) |
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| BB/N503629/1 | 10/10/2015 | 09/10/2019 | |||
| 1632663 | Studentship | BB/N503629/1 | 07/09/2015 | 06/09/2019 |
| Description | Through this award a new 3D organotypic model of skin ageing has been developed using replicative senescent fibroblasts. We have discovered that replicative senescent fibroblasts can be used to produce a skin aging phenotype, and that there are clear phenotypic differences between proliferating and senescent fibroblasts in 2D and 3D. |
| Exploitation Route | Further investigation into the mechanisms involving the skin ageing phenotype and replicative senescence can be conducted using both the data produce and the organotypic modelling technique developed within the project. |
| Sectors | Healthcare,Pharmaceuticals and Medical Biotechnology |
| Description | Blizard STARS |
| Form Of Engagement Activity | Participation in an open day or visit at my research institution |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Schools |
| Results and Impact | Blizard STARS (Science Training for Aspiring Research Scientists) is a one week widening participation programme that offer 9 A'level pupils with an interest in science - whether they're thinking of medical research, dentistry, or are generally curious about lab work - to perform experiments, learn techniques and discover the research environment.This is also an important opportunity for our postgraduate students and senior researchers (we call them STARS Facilitators) to engage with the students to reinforce their science communication and teaching skills. The PDRA employed on this award has supported the organisation and delivery on this programme. The week long programme is complemented with an 'Insight morning' open to 20-25 A'Level pupils. Our STARS have received awards at an annual ceremony held at the House of Lords, and in 2018, Blizard STARS received the Institute's award for 'Outstanding Contribution to Public Engagement'. As recognition, this scheme is now delivered under the Mayor's Fund for London Umbrella. Our 2019 programme is scheduled for July of this year. |
| Year(s) Of Engagement Activity | 2017,2018,2019 |