The structural and functional analysis of transcription factor E4bp4
Lead Research Organisation:
Imperial College London
Department Name: Life Sciences
Abstract
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Organisations
People |
ORCID iD |
| Hugh Brady (Primary Supervisor) |
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| BB/M011178/1 | 30/09/2015 | 25/02/2025 | |||
| 1657180 | Studentship | BB/M011178/1 | 02/10/2015 | 29/09/2019 |
| Description | From initial submission of the award the project has changed slightly. I am now looking into whether E4bp4 has a major role in human NK cell development from cord blood CD34+ hematopoeitic progenitor cells. This is achieved via targeting the gene prior to in vitro NK cell differentiation using a lentiviral based CRISPR- Cas9 system. One important finding is that as we achieve a biallelic E4bp4 gene knockout cell, these cells cannot clonally expand and differentiate into NK cells. This suggests it does play a vital role in NK cell development. To validate this finding and ensure our system works, we are also targeting genes not associated with NK cell development. |
| Exploitation Route | Findings for this work can provide alternative and improved strategies for NK cell based immunotherapy. Also compounds that are designed to interact with components of the genes signalling mechanism an be looked into as an additional strategy. This can potentially avoid the use of genetically manipulating these cells and make it slightly easier to develop functional NK cells. |
| Sectors | Healthcare Manufacturing including Industrial Biotechology Pharmaceuticals and Medical Biotechnology |