Developing Eubacterium limosum as a robust chassis organism
Lead Research Organisation:
University of Nottingham
Department Name: School of Life Sciences
Abstract
The main aim of the PhD project is to engineer an Eubacterium limosum strain(s) to produce valuable chemicals from methanol. The first goal is to improve our ability to ferment E. limosum in a bioreactor, developing a robust method for continuous fermentation and using it to not only assay phenotypes from created mutants, but also to act as a form of directed evolution to allow for such things as adaption to feeding on gas alone. Deletions of non-essential genes (guided by a genome scale model) will be performed to gain a better understanding of genomic function, likely by using fermentation to assess product yields. Mutants would be created to improve product yields (of existing products) and/or introduce new pathways to produce more valuable chemicals.
| Description | ERACoBioTech Biometchem Sustainable Production of Added Value Chemicals from SynGas-derived Methanol Through Systems and Synthetic Biology Approaches |
| Organisation | Goethe University Frankfurt |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | Each team group of the ERACoBioTech Biometchem project was responsible for the contribution of data. The University of Nottingham was, in addition to Responsible Research and Innovation and organisation of the collaboration, dealt with tool building. The CRISPR Cas9 system used in this project was developed at the University of Nottingham and the deletions I produced for this collaboration were created using this system. Nottingham was to also develop this system further and improve upon it, and use TraDIS to determine gene essentiality. |
| Collaborator Contribution | Each team group of the ERACoBioTech Biometchem project was responsible for the contribution of data. Institut national des sciences appliquées de Toulouse was responsible for the creation of a genome scale model and metabolic analysis. University of Ulm was responsible for implementation of synthetic pathways to produce products using methanol as a feedstock, ideally GABA and 1,3 butanediol. Goethe University Frankfurt was responsible for protein analysis and enzyme kinetics. |
| Impact | As of the time of this submission no outputs or outcomes have arisen as the collaboration is still active and in progress. |
| Start Year | 2018 |
| Description | ERACoBioTech Biometchem Sustainable Production of Added Value Chemicals from SynGas-derived Methanol Through Systems and Synthetic Biology Approaches |
| Organisation | National Institute of Applied Sciences of Toulouse |
| Country | France |
| Sector | Academic/University |
| PI Contribution | Each team group of the ERACoBioTech Biometchem project was responsible for the contribution of data. The University of Nottingham was, in addition to Responsible Research and Innovation and organisation of the collaboration, dealt with tool building. The CRISPR Cas9 system used in this project was developed at the University of Nottingham and the deletions I produced for this collaboration were created using this system. Nottingham was to also develop this system further and improve upon it, and use TraDIS to determine gene essentiality. |
| Collaborator Contribution | Each team group of the ERACoBioTech Biometchem project was responsible for the contribution of data. Institut national des sciences appliquées de Toulouse was responsible for the creation of a genome scale model and metabolic analysis. University of Ulm was responsible for implementation of synthetic pathways to produce products using methanol as a feedstock, ideally GABA and 1,3 butanediol. Goethe University Frankfurt was responsible for protein analysis and enzyme kinetics. |
| Impact | As of the time of this submission no outputs or outcomes have arisen as the collaboration is still active and in progress. |
| Start Year | 2018 |
| Description | ERACoBioTech Biometchem Sustainable Production of Added Value Chemicals from SynGas-derived Methanol Through Systems and Synthetic Biology Approaches |
| Organisation | University of Ulm |
| Country | Germany |
| Sector | Academic/University |
| PI Contribution | Each team group of the ERACoBioTech Biometchem project was responsible for the contribution of data. The University of Nottingham was, in addition to Responsible Research and Innovation and organisation of the collaboration, dealt with tool building. The CRISPR Cas9 system used in this project was developed at the University of Nottingham and the deletions I produced for this collaboration were created using this system. Nottingham was to also develop this system further and improve upon it, and use TraDIS to determine gene essentiality. |
| Collaborator Contribution | Each team group of the ERACoBioTech Biometchem project was responsible for the contribution of data. Institut national des sciences appliquées de Toulouse was responsible for the creation of a genome scale model and metabolic analysis. University of Ulm was responsible for implementation of synthetic pathways to produce products using methanol as a feedstock, ideally GABA and 1,3 butanediol. Goethe University Frankfurt was responsible for protein analysis and enzyme kinetics. |
| Impact | As of the time of this submission no outputs or outcomes have arisen as the collaboration is still active and in progress. |
| Start Year | 2018 |