Antibiotic Resistance
Lead Research Organisation:
Imperial College London
Department Name: Dept of Medicine
Abstract
Characterise a target for a novel type of anti-bacterial therapeutic.
People |
ORCID iD |
| Kam Po Ha (Student) |
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| MR/R502376/1 | 03/09/2017 | 01/01/2025 | |||
| 2042385 | Studentship | MR/R502376/1 | 03/10/2015 | 30/09/2018 | Kam Po Ha |
| Title | Reporter strain to detect the DNA damage response in MRSA |
| Description | This MRSA strain produces GFP in response to induction of the SOS pathway, which is indicative of DNA damage. This tool can be used to detect induction of this pathway under different conditions, e.g. antibiotics, host immune factors, environmental factors. |
| Type Of Material | Cell line |
| Year Produced | 2019 |
| Provided To Others? | Yes |
| Impact | Has been used to confirm findings in multiple papers (published), and will be used in the future for screening of novel compounds for antibiotic therapy. |
| Title | RexAB enzyme activity assays |
| Description | Development of two specialised assays to measure the helicase and nuclease activities of the staphylococcal RexAB protein, which is a viable drug target for novel antibiotics. These assays were adapted for testing with various compounds and antibiotics, to determine their effect on the protein. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2018 |
| Provided To Others? | No |
| Impact | Was used to confirm the function of the DNA damage repair protein RexAB. These assays will also be used to confirm hits from screens to detect novel antibiotic compounds against RexAB. |
| Description | Development of small-molecule inhibitors of RexAB |
| Organisation | Imperial College London |
| Department | Department of Chemistry |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | Provided background data and biological tools for testing of compounds. |
| Collaborator Contribution | Synthesis and optimisation of a panel of small molecules for testing with RexAB |
| Impact | Publication of a paper 4x Masters student projects Multi-disciplinary - Infectious Disease, Chemistry |
| Start Year | 2017 |
| Description | Imperial Festival stall |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Public/other audiences |
| Results and Impact | Designed and ran a game to describe the project to the general public (now called the Great Exhibition Road Festival). This led to opportunities for other engagement events for the rest of the group, including participation in the Science Museum Lates. |
| Year(s) Of Engagement Activity | 2017,2018,2019 |