Elucidating the molecular mechanism of DNA-end resection and its role in the Double-Strand-Break (DSB) repair pathway choice.
Lead Research Organisation:
Institute of Cancer Research
Department Name: Division of Cancer Biology
Abstract
Aim 1. A commitment to HR: defining the role of EXD2 in DSB repair pathway choice
Aim 2. In vitro biochemistry analyses of the effect of end resection on the DNA binding of NHEJ factors.
Aim 2. In vitro biochemistry analyses of the effect of end resection on the DNA binding of NHEJ factors.
People |
ORCID iD |
| Caroline Clarke (Student) |
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| MR/R01583X/1 | 01/10/2018 | 30/09/2025 | |||
| 2119094 | Studentship | MR/R01583X/1 | 01/10/2018 | 30/09/2022 | Caroline Clarke |
| Description | Tandem mass tag mass spectrometry screen (co-immunoprecipitation) |
| Organisation | Institute of Cancer Research UK |
| Country | United Kingdom |
| Sector | Academic/University |
| PI Contribution | My lab optimised the conditions for the co-immunoprecipitations, and prepared the samples for the screen including the immunoprecipitations, tryptic digest and tandem mass tag labelling. |
| Collaborator Contribution | The proteomic team led by Jyoti Choudhary advised on how to carry out the necessary tryptic digest and tandem mass tag labelling. In addition, they ran the samples on the mass spectrometer and carried out the search for peptide hits. |
| Impact | The mass spectrometry screen dataset. It is multidisciplinary including work in molecular/cellular biology, proteomics analysis and biochemical methods. |
| Start Year | 2018 |