Developing a zebrafish model for a novel human neurodevelopmental disorder.
Lead Research Organisation:
University of Manchester
Department Name: School of Biological Sciences
Abstract
We have recently discovered a novel human disorder caused by mutations in the EIF5A1 gene resulting in developmental problems, small head size and craniofacial defects in children [1]. EIF5A1 is unique because it is the only known protein to contain the amino acid hypusine and is essential for proper synthesis of a set of important proteins[2]. Using yeast and zebrafish model systems we have shown how EIF5A1 mutations lead to impaired eIF5A function and cause phenotypes consistent with the disease. Interestingly, in the same model systems we have shown that a specific food supplement may be a potential treatment for this disease[1].
Project
The normal expression pattern of eIF5A1 in developing zebrafish will be studied using in-situ hybridisation and fluorescent microscopy. CRISPR/Cas will be used to generate zebrafish lines with human EIF5A1 mutations. These fish will be characterized for brain and craniofacial defects and their tissues will be studied using Mass-spectrometry, RNA-seq and biochemical assays of eIF5A functions. Phenotype rescue studies of the mutant zebrafish will be performed. An international patient registry will be developed for further studying the human phenotype and to prepare a cohort for future treatment trials.
Outcome
The student will generate and characterise vertebrate models of a novel human disorder at the interface of fundamental biology and translational precision medicine. The student will learn several fundamental interdisciplinary and quantitative skills, as well as gaining significant experience of studying whole organism physiology in context of human disease.
Project
The normal expression pattern of eIF5A1 in developing zebrafish will be studied using in-situ hybridisation and fluorescent microscopy. CRISPR/Cas will be used to generate zebrafish lines with human EIF5A1 mutations. These fish will be characterized for brain and craniofacial defects and their tissues will be studied using Mass-spectrometry, RNA-seq and biochemical assays of eIF5A functions. Phenotype rescue studies of the mutant zebrafish will be performed. An international patient registry will be developed for further studying the human phenotype and to prepare a cohort for future treatment trials.
Outcome
The student will generate and characterise vertebrate models of a novel human disorder at the interface of fundamental biology and translational precision medicine. The student will learn several fundamental interdisciplinary and quantitative skills, as well as gaining significant experience of studying whole organism physiology in context of human disease.
Organisations
People |
ORCID iD |
Graham Pavitt (Primary Supervisor) | |
Carolina Stamboulid (Student) |
Studentship Projects
Project Reference | Relationship | Related To | Start | End | Student Name |
---|---|---|---|---|---|
MR/N013751/1 | 01/10/2016 | 30/09/2025 | |||
2456448 | Studentship | MR/N013751/1 | 01/10/2020 | 30/09/2024 | Carolina Stamboulid |