Nano-Probes for Point of Care Diagnostics
Lead Research Organisation:
University of Warwick
Department Name: Chemistry
Abstract
An emerging diagnostic platform is that based upon plasmonic nanoparticles, particularly gold nanoparticles. Gold nanoparticles are robust biosensors with several key features making them ideal for point of care and monitoring applications. i) Gold nanoparticles are relatively inexpensive (e.g. the red strip in home pregnancy tests is composed of gold particles and they wholesale for ~ 20 pence each); ii) Gold nanoparticles are established for clinical and vetinary use in lateral flow assays (e.g. pregnancy test); iii) Gold nanoparticles display a distinct red-blue colour change when binding biological targets in solution. This latter point will be the focus of this project. When the ligand for a pathogen is immobilized onto the particle surface it produces a red-blue colour shift (visible by the naked eye) when the pathogen is present and is hence ideally suited to point of care devices.
An emerging diagnostic platform is that based upon plasmonic nanoparticles, particularly gold nanoparticles - due to their strong colouration and unique change in optical properties (colour) upon binding targets they are ideal sensors for 'easy point of care diagnostics' which the GibsonGroup have used for bacterial and toxin detection. This project will combine the expertise in the GibsonGroup in developing new biosensors (Molecular Biosystems, 2016, 12, 341, J. Mater. Chem. B., 2016, 4, 3046) with that of Iceni Diagnostics; a young and agile UK Biotech.
The project will seek to develop these nanosensors towards validated targets for detection, making use of precision polymer and materials chemistry, with modern glycobiology and nano science.
An emerging diagnostic platform is that based upon plasmonic nanoparticles, particularly gold nanoparticles - due to their strong colouration and unique change in optical properties (colour) upon binding targets they are ideal sensors for 'easy point of care diagnostics' which the GibsonGroup have used for bacterial and toxin detection. This project will combine the expertise in the GibsonGroup in developing new biosensors (Molecular Biosystems, 2016, 12, 341, J. Mater. Chem. B., 2016, 4, 3046) with that of Iceni Diagnostics; a young and agile UK Biotech.
The project will seek to develop these nanosensors towards validated targets for detection, making use of precision polymer and materials chemistry, with modern glycobiology and nano science.
Publications
Laezza A
(2020)
Protecting Group Free Synthesis of Glyconanoparticles Using Amino-Oxy-Terminated Polymer Ligands.
in Bioconjugate chemistry
Richards SJ
(2020)
Introducing affinity and selectivity into galectin-targeting nanoparticles with fluorinated glycan ligands.
in Chemical science
Georgiou PG
(2020)
"Tuning aggregative versus non-aggregative lectin binding with glycosylated nanoparticles by the nature of the polymer ligand".
in Journal of materials chemistry. B
Richards S
(2020)
Polymer-Stabilized Sialylated Nanoparticles: Synthesis, Optimization, and Differential Binding to Influenza Hemagglutinins
in Biomacromolecules
Baker AN
(2020)
The SARS-COV-2 Spike Protein Binds Sialic Acids and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device.
in ACS central science
Baker A
(2021)
Glycan-Based Flow-Through Device for the Detection of SARS-COV-2
in ACS Sensors
Baker A
(2021)
Correction to "The SARS-COV-2 Spike Protein Binds Sialic Acids, and Enables Rapid Detection in a Lateral Flow Point of Care Diagnostic Device".
in ACS central science
Baker AN
(2022)
Lateral Flow Glyco-Assays for the Rapid and Low-Cost Detection of Lectins-Polymeric Linkers and Particle Engineering Are Essential for Selectivity and Performance.
in Advanced healthcare materials
Studentship Projects
Project Reference | Relationship | Related To | Start | End | Student Name |
---|---|---|---|---|---|
BB/M01116X/1 | 30/09/2015 | 31/03/2024 | |||
1898615 | Studentship | BB/M01116X/1 | 01/10/2017 | 30/12/2021 | Alexander Baker |
Description | Cuppa Science (Pint of Science National) |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | National Pint of Science Event |
Year(s) Of Engagement Activity | 2020 |
Description | International news interview (Associate Press) |
Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
Part Of Official Scheme? | No |
Geographic Reach | International |
Primary Audience | Public/other audiences |
Results and Impact | Interview with AP on covid diagnostic work |
Year(s) Of Engagement Activity | 2020 |
Description | Interview for National News (BBC) |
Form Of Engagement Activity | A press release, press conference or response to a media enquiry/interview |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | Interview by BBC on Covid diagnostic research |
Year(s) Of Engagement Activity | 2020 |
URL | https://www.bbc.co.uk/programmes/m000kf7n |
Description | NerdNite event |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Public/other audiences |
Results and Impact | Online NerdNite invited speaker |
Year(s) Of Engagement Activity | 2021 |
Description | Pint of Science 2019 |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Public/other audiences |
Results and Impact | Pint of Science event between Coventry and Warwick Universities |
Year(s) Of Engagement Activity | 2019 |
Description | Summer camp Science Track |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | I run a 3-day science track for primary school children, we do demonstrations and hands on science |
Year(s) Of Engagement Activity | 2017,2018,2019 |
Description | The Training Partnership - Chemistry in Action A-level |
Form Of Engagement Activity | A talk or presentation |
Part Of Official Scheme? | No |
Geographic Reach | National |
Primary Audience | Schools |
Results and Impact | Over three events ~2500 A-level students were reached with talk on LFD technology |
Year(s) Of Engagement Activity | 2020,2021 |
Description | Warwick Christmas Lecture |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Regional |
Primary Audience | Schools |
Results and Impact | Presented to over 1000 students attended and engaged in a general evening of Science |
Year(s) Of Engagement Activity | 2019 |
URL | https://www.warwickartscentre.co.uk/whats-on/2019/warwick-christmas-lectures-2019/ |