Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and Wellbeing

Lead Research Organisation: Imperial College London
Department Name: Dept of Chemistry

Abstract

New and novel measurement tools and technologies enable modern biological and biomedical research to continue to move forward at a rapid rate. Detecting disease and evaluating the effects of drugs all rely on measurement technologies, and the limitations in our ability to measure things rapidly, accurately and cheaply is often the bottleneck in many research areas. Similarly, advances in industrial processes and clinical methods are often dependent on matching advances in measurement technology. In this programme two leading research centres from Glasgow University and Imperial College London join forces to develop new techniques and tools in order to improve existing approaches to detect and quantify the effects of protein oxidative damage in biological samples. Protein oxidative damage is one of the components of ageing and can cause a range of serious medical problems. As part of this project we will improve the ways in which biological samples are delivered into a range of analytical platforms such as mass spectroscopy and protein microarrays by employing novel innovative approaches such as acoustic sound waves, emulsion droplets and microfludics. Chemical tools and probes that seek out and detect damaged proteins will be constructed and subsequently incorporated into the technologies mentioned above. These technologies will be used to study the dynamics of protein damage in space and time. Another part of the programme develops high throughput analysis tools of various forms all of which use minaturisation methods to allow for the analysis of as many proteins as possible, as rapidly as possible and with as much detail as possible. It is anticipated that this multidisciplinary programme will create the necessary analytical platforms to overcome a technological gap that is holding back biological and medical discoveries in this research area that is vital for fostering our understanding of human health and ageing. It also has the potential to facilitate medical interventions to alleviate the symptoms of ageing and age-related illness. Given the importance of these issues for the general population, not just of the UK but worldwide, the impact of this programme will be significant and sustained for several years in terms of academic output as well as industrial wealth generation. In particular, novel drug targets and indeed drugs, as well as improved diagnostic techniques to monitor and assess human health and ageing are possible outcomes in the near future. Given the importance of oxidative damage in crop production it is likely that our programme will also produce tools that benefit the agrochemical industry, making this proposal a truly discipline crossing venture with respect to the sciences involved and the impact generated.

Planned Impact

This project will be of benefit to both the academic and industrial communities and is aligned with three of the EPSRC's strategic areas (Nanoscience through engineering to application, Ageing-Lifelong Health and Wellbeing, and Towards Next Generation Healthcare). The programme which capitalises upon seven-state-of the-art technologies developed within the ICB DTC and C&PT DTCs will realise a number of key advances with broad-reaching application. It is directly focused on the development of tools that will enable drug discovery and disease proteomics, with improved healthcare as the long-term goal. The advances that will benefit both sectors include technical improvements in mass spectrometry, multidimensional spectroscopy, chemical probes, protein arrays, microfluidics and proteomic activity profiling. In addition, the tools and techniques under development here have the potential to provide enhanced capabilities for end-users in basic biology, biomarker discovery, diagnostics and drug discovery. These applications can be directly exploited by the pharmaceutical industry, medical sector, biotechnology SMEs and instrumentation manufacturers. The project builds on our existing doctoral training programmes and will provide training of a cohort of PDRAs and students in the important area of post-genomic technology and novel analytical tools. There remains an unmet need for highly capable multidisciplinary scientists with genuine expertise across more than one discipline. Our programme will produce future designers and developers of new measurement techniques and strategies via a cohort who understand both a range of technologies and the details of the biological and biomedical challenges to be addressed. The transferable skills we will provide will include courses on systems integration, entrepreneurship and exploitation, allowing them to translate and exploit their research both during the project and their future careers. The focus of the programme is concerned with providing tools for the better study of and intervention into oxidative damage of proteins, which is one of the molecular components of ageing. There are obvious benefits to the general population of research that has the potential to impact on the adverse effects of ageing and improve quality of life. UK industry will benefit from the scientific and technical innovations we will produce, with a 5-10 year timeframe for commercial realisation. The diagnostics tools that we will develop will not only be able to detect the changes associated with disease or ageing but also, and most importantly, to monitor their progress during the drug development process and subsequent therapy (10 yrs). The economic imperative is for medicine to be more preventative; these tools will play a vital role by facilitating the identification of illness in its early stages, allowing for early intervention to reduce permanent damage (10 yrs) and alleviating associated healthcare costs. In the context of drug discovery, the ability to understand and study the enzyme active sites and peripheral damage to easily oxidised signalling systems paves the way for novel intervention strategies either to mitigate aging effects or to target these proteins as part of an intervention for other diseases. Our technologies have the potential both to identify and to validate targets and biomarkers, as well as testing for side effects of drug candidates (5-10 yrs). Individual elements such as the MAC chips and 2DIR will be exploitable for clinical diagnostics and drug discovery on shorter time scales (ca. 3-5 yrs) and the proteomic technologies we will develop can be applied to any disease, including cancer, and cardiovascular diseases. The result will be improved healthcare as well as enhanced economic competitiveness of the United Kingdom by generating wealth and potential future employment.

Publications

10 25 50
 
Title Large Cell picture from Royal Society Summer Exhibition 
Description Large backlit image of a fluorescent cell used as the back panel of our stand at the RSSE. 
Type Of Art Artwork 
Year Produced 2015 
Impact Since the end of the exhibition the panel has been moved to Imperial college where it will become a permanent feature in the Chemistry department. 
 
Title Model Mass Spectrometer 
Description A model showing how a magnetic field can be used to sort different sized objects. Metal beads of different sizes are launched down a ramp and pass near a strong magnet. The magnet changes the path of these beads and directs them to one of many destinations. In effect, it demonstrates the main principle behind mass spectroscopy and is a great educational tool. 
Type Of Art Artistic/Creative Exhibition 
Year Produced 2014 
Impact Used in a number of public engagement events including the 2014 World science fiction convention in London. 
 
Title PROXO Workstream Videos 
Description These videos show the different workstreams of our project and how they intercalate. They are available on our webpage. 
Type Of Art Film/Video/Animation 
Year Produced 2014 
Impact These videos are a really easy way for new collaborators to familiarise themselves with our project and research. 
 
Title PROXO video 
Description The PROXO video describes the main focus of the research and is available on the proxomics webpage. 
Type Of Art Film/Video/Animation 
Year Produced 2014 
Impact The video is a really easy means to tell people what our project is about and we have had lots of positive feedback from collaborators and conference attendees. 
URL http://www.proxomics.ac.uk/
 
Title Proxomics.ac.uk 
Description Our Proxomics webpage is primarily a tool to engage the public and collaborators in our research and disseminate our progress and our activities. It features many videos and graphical illustrations on how we have arranged our multidisciplinary work streams to work together. 
Type Of Art Artefact (including digital) 
Year Produced 2012 
Impact We've obtained lots of collaborative interest through the information advertised on our website. 
URL http://www.proxomics.ac.uk/
 
Title The Cell Panel 
Description The Cell panel was created for the RSSSE exhibition. It is an interactive cell that shows how protein interactions can affect cell signalling and how damage to signalling proteins can result in cancer. 
Type Of Art Artwork 
Year Produced 2015 
Impact The cell panel was well visited at The RSSSE and drew a lots of interest from the younger visitors. 
 
Description We have developed a suite of tools and technologies able to study the effect of protein oxidation and oxidative stress on human biology with particular reference to the development of a range of diseases. These are now being used for biological and biomedical studies in follow-up projects.
Exploitation Route Many of the methods developed are in use for both academic projects but also for collaborative work with industry and clinical researchers.
Sectors Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology

 
Description The innovative tools and techniques of this project have opened up industry collaborations and are continuously sought after when presented at in-the-field as well as cross-disciplinary conferences. Some of the methods are being used in follow up applications with both industry and clinical researchers.
Sector Healthcare,Pharmaceuticals and Medical Biotechnology
Impact Types Economic

 
Description CASE Award
Amount £92,000 (GBP)
Funding ID BB/J012939/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start  
 
Description COST Action Award
Amount £1,100 (GBP)
Organisation European Cooperation in Science and Technology (COST) 
Sector Public
Country European Union (EU)
Start  
 
Description DTA Awards, PhD bursaries
Amount £240,000 (GBP)
Funding ID EP/J500239/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start 10/2011 
End 09/2015
 
Description EPSRC Fellowship
Amount £1,538,918 (GBP)
Funding ID EP/K027611/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start  
 
Description Farmed Animal Disease and Health (FADH) [2013]
Amount £437,440 (GBP)
Funding ID BB/L00464X/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start  
 
Description Impact Acceleration Award
Amount £75,066 (GBP)
Funding ID EP/K503733/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start  
 
Description Institutional Strategic Support Fund: Network of Excellence Scheme
Amount £100,000 (GBP)
Organisation Wellcome Trust 
Department Wellcome Trust Institutional Strategic Support Fund
Sector Charity/Non Profit
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start  
 
Description Knowledge Transfer Secondments; Impact Acceleration Account
Amount £46,344 (GBP)
Funding ID EP/K503733/1 
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Academic/University
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start  
 
Description Lowe Syndrome Trust
Amount £330,000 (GBP)
Organisation Lowe Syndrome Trust 
Sector Charity/Non Profit
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start  
 
Description SSSC
Amount £40,000 (GBP)
Organisation Government of Scotland 
Department Scottish Funding Council
Sector Public
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start  
 
Description Social entrepreneurship funding
Amount £500 (GBP)
Organisation UnLtd 
Sector Charity/Non Profit
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start  
 
Description Technology Strategy Board (TSB) [2011-2014]
Amount £243,768 (GBP)
Funding ID BB/L011336/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start  
 
Description Technology Strategy Board (TSB) [2011-2014]
Amount £243,768 (GBP)
Funding ID BB/L011336/1 
Organisation Biotechnology and Biological Sciences Research Council (BBSRC) 
Sector Public
Country United Kingdom of Great Britain & Northern Ireland (UK)
Start  
 
Title 2DIR spectrometer 
Description Construction of 2DIR Spectrometer and applying it to the detection of oxidized protein side chains. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact 2DIR has been able to successfully detect oxidised forms of proteins and this has led to publication. 
 
Title Chip surface passivation protocols 
Description To limit the amount of non-specific binding to surface to maintain sensitivity silane, PEG and 3D polymer based surface chemistry was investigated. It was determined that silane based chemistry is appropriate for DNA assays whereas PEG based chemistries are better suited for protein assays. 
Type Of Material Improvements to research infrastructure 
Provided To Others? No  
Impact These passivated slides are required for use with the MAC chip platform a vital part of the Proxomics project. 
 
Title Humidity cell for 2DIR experiments 
Description A humidity cell was made from a cheap optical mount, a CaF2 window, a glass coverslip, PTFE spacers and a rubber o-ring. The biological sample of interest is placed on the glass slide along with several drops of saturated salt solution, which enable a constant humidity to be maintained inside the cell for at least a month. This ensures that the water content of the biological sample of interest remains constant throughout a series of 2DIR experiments. 
Type Of Material Improvements to research infrastructure 
Provided To Others? No  
Impact This humidity cell is much smaller than commercial humidity cells and is easier to mount on our 2DIR sample stage, allowing for a quicker and more reproducible experimental set-up. It is also much cheaper than commercial cells, which means that we have made 5 of these humidity cells containing different biological samples for the same price as just 1 commercial cell. This leads to higher throughput in our 2DIR experiments. 
 
Title Microfluidic Antibody Capture Chips 
Description Designed and built microfluidic antibody capture chips, an all optical platform for the quantification of protein in single cells. Cells are loaded into these devices, transported to analysis chambers using an optical trap and then ruptured with the use of a high powered optical lysis laser. The intracellular contents are then quantified by the use of a miniaturized ELISA format. Proteins bind to antibodies on the surface of the device and a secondary fluorescent antibody, present in solution completes the sandwich system. Total Internal Reflection Fluorescence (TIRF) microscopy is then used to detect and count the amount of protein that has bound to the antibody spot. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact The use of MAC chips dominates an entire work stream in the Proxomics project and has resulted in a number of publications. 
 
Title Microfluidic antibody capture (MAC) chip design 
Description A microfluidic antibody capture chip was designed to allow to isolation of single cells from a population, their subsequent lysis and binding of a protein or proteins of interest to an antibody patch. Separate designs for the isolation of single, few (1-5) and many (10-100) cells from flow were also made. 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact MAC chips dominate an entire workstream of the proxomics project and have resulted in a number of publications. 
 
Title Microfluidic chips design for handling Smart Droplet Microtools and cells 
Description Microfluidic chips design for handling Smart Droplet Microtools and cells 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact Smart Droplet microtools and the associated platform have led to the publication of several papers. 
 
Title Opto/Thermoporation of single cells 
Description The discovery that poly-silicon can be used to cause cell membrane phase changes when irradiated with 1070nm laser light the technique. 
Type Of Material Improvements to research infrastructure 
Year Produced 2015 
Provided To Others? Yes  
Impact Has the ability to revolutionise cell transfection. Can selectively permeable single cells in a microfluidic environment which can then take up a variety of otherwise cell impermeable reagents. 
 
Title Plasmids tagging VHR with HA and Flag 
Description Plasmids pcDNA-3.1-Flag-VHR and pcDNA-3.1-HA-VHR, to express N-terminal tagged VHR. 
Type Of Material Biological samples 
Provided To Others? No  
Impact Enabled tagged VHR to be expressed in cell lines and detected using different antibodies 
 
Title Protocols for cell handling onto chips 
Description Cells were detached from flasks using gentle tryptic agents and filtered using a nylon mesh. Cells are suspended in media using a CO2 independent buffer. Cells are delivered via capillary tubing. 
Type Of Material Improvements to research infrastructure 
Provided To Others? No  
Impact Cells are used in microfluidic devices for both the MAC chip and SDM platforms both of which have resulted in publications 
 
Title SAW Cell Lysis 
Description A chemical free lysis method for use in lab on a chip device. Surface acoustic waves use to mechanically lyse a cell sample for downstream analysis. 
Type Of Material Technology assay or reagent 
Year Produced 2014 
Provided To Others? Yes  
Impact Chemcial free lysis based on acoustic force can be implemented in point-of-care devices and easily coupled to downstream diagnostic applications 
 
Title SAW tunnels to spatially position a SAW beam at a microfluidic chamber/channel 
Description A problem with SAW streaming in PDMS mirochannels is the damping effect of the PDMS wall. I used a dry channel to allow the SAW to effectively tunnel through the PDMS wall. This concept also allows for the precise positioning of the Saw in the chamber 
Type Of Material Improvements to research infrastructure 
Provided To Others? No  
Impact A potential useful tool for designing and realizing SAW acoustophoresis and streaming devices 
 
Title SAWN LC/MS 
Description Combination of the SAW nebulsation with mass spectroscopy for a more gentle sample prep 
Type Of Material Technology assay or reagent 
Year Produced 2012 
Provided To Others? Yes  
Impact The technique may potential be used in standard proteomic mass spectroscopy work flows where electrospray is deemed unsuitable 
 
Title Single Cell Antibody printing 
Description All antibodies were printed by an OmniGrid Micro microarrayer (Digilab, UK) using 946MP2 pins (ArrayIt, USA). Spotting solution was optimised for DNA, antibody, protein or cell-lysate printing. 
Type Of Material Improvements to research infrastructure 
Provided To Others? No  
Impact Antibody printing is a vital step in the MAC chip platform. This platform is a vital part of the proxomics project and has resulted in a number of publications. 
 
Title Single Cell Trapping array 
Description A microfluidic device optimised for capturing cells in an array for subsequent single cell analysis 
Type Of Material Technology assay or reagent 
Provided To Others? No  
Impact Will be of interest in the field of microfluidics and single cell analysis. Poster describing the method was shortlisted for poster prize at Microfluidics 2014 conference 
 
Title Single Cell lysis protocols 
Description A technique using a short pulsed laser is employed to lyse single cells. Through a series of physical processes a cavitation bubble expands from the focal point of the laser. The shearing forces experienced by the cell placed approximately 10_m away disrupts the cell and liberates intracellular constituents for analysis. It has been demonstrated that the cell may be fully (all membranes ruptured including nuclear membrane) or partially (only plasma membrane ruptured preserving the nucleus intact) lysed. The optical lysis process is extremely fast whereby the cell is disrupted within a few microseconds. 
Type Of Material Improvements to research infrastructure 
Provided To Others? No  
Impact Optical lysis is a vital part of the Microfluidic antibody capture platform which has resulted in a number of publications. 
 
Title Sorting of Low Abundance Cells from Small Clinical Samples for downstream single cell analysis 
Description We have shown that it is possible to use the laser trap in combination with surface markers to select cells of interest from a heterogeneous mix. The cells are selected by type and then moved into the analysis section of the MAC chip for assessment of their signalling status as read out by protein copy number and PTM condition. 
Type Of Material Improvements to research infrastructure 
Year Produced 2016 
Provided To Others? Yes  
Impact We are now in a position to demonstrate the utility of this approach in clinical contexts. A pilot project with ICR has been completed and a paper submitted and is under review. Two other projects are underway, one with NHLI/Brompton Hospital on COPD and another with Kings College on the activation status of immune cells in human tumours. 
 
Title Sorting of different sized particles using a combination of SAW streaming and acoustophoresis 
Description Looked at using tunnels to create a vortex that can trap and concentration small particles (micrometer diam). Bigger particles are pushed away by acoustic radiation force (10 micro meters) 
Type Of Material Improvements to research infrastructure 
Provided To Others? No  
Impact Could be an interesting way of concentrating and refining very small cells, for example verticals and exosomes in a blood sample. What would be particularly novel would be the use of streaming vortices to trap and concentrate very small particles /cells/vesicles against a sheath flow 
 
Title TIRF-FLIM system 
Description Combined total internal reflection fluorescence lifetime imaging system for improved resolution to conventional fluorescence lifetime imaging techniques 
Type Of Material Improvements to research infrastructure 
Provided To Others? No  
Impact Enhanced the capabilities of standard fluorescence lifetime imaging through improved axial resolution. Technology is of particular use for investigations of dynamics in biological membranes. 
 
Title A simulation of the SAW induced streaming in a microfluidic chamber/channel 
Description Created a novel simulation for describing the SAW streaming inside microfluidic volumes. This involved analytical and FEM parts 
Type Of Material Computer model/algorithm 
Provided To Others? No  
Impact This should be a useful too for researchers designing SAW devices with the intention of creating streaming flows in mirofluidic chambers. With this simulation it was possible to create scaling laws between frequency and the chamber length/height which can guide the optimum selection of SAW frequency for a given microfluidic set-up 
 
Title Approximate Bayesian Computation (ABC) 
Description Parameter inference algorithm for stochastic simulations 
Type Of Material Computer model/algorithm 
Provided To Others? No  
Impact Has been used to develop a model of p53 expression and compare it with experimental data. 
 
Title Capture Assay Model 
Description A software that models the scaling advantages and constraints in miniaturized capture assays for single cell proteomics 
Type Of Material Computer model/algorithm 
Provided To Others? No  
Impact Used in group to predict assay behaviour and inform assay validation 
 
Title GDSC PALM Counting Algorithm 
Description Single molecule localisation counting algorithm. 
Type Of Material Computer model/algorithm 
Provided To Others? No  
Impact Counting algorithm essential to the MAC chip platform. Replaces older counting algorithm and has provided better results. 
 
Title Micro PIV and vibrometer measurements of SAW streaming in microfluidic chamber 
Description A combination of micro PIV and vibrometer measurements that were collected to validate the simulation (see above) for a range of different SAW frequencies and powers 
Type Of Material Database/Collection of data 
Provided To Others? No  
Impact Helps to enhance knowledge/models of SAW streaming in microfluidic chambers/channels 
 
Description Ajay Mahalka 
Organisation Aalto University
Country Finland, Republic of 
Sector Academic/University 
PI Contribution Mass spectrometry analysis of Phosphalipase A2 modified with PoxnoPC
Collaborator Contribution n/a
Impact n/a
Start Year 2012
 
Description Ben Tiller and Michael Dent 
Organisation Imperial College London
Department Department of Chemistry
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution Use of fluorescence microscopy/spectroscopy and operational expertise.
Collaborator Contribution Synthesised molecular rotor molecules to be investigated.
Impact Led to a joint publication between Glasgow and Imperial with the full details in the publications section of ResearchFish DOI:10.1039/C5CP01937K
Start Year 2014
 
Description Dr Corinne Spickett 
Organisation Aston University
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution Dr Corinne Spickett is a collaborator and co-I on the grant 'Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and Wellbeing'.
Start Year 2011
 
Description Dr Ekaterina McKenna 
Organisation University of Glasgow
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution n/a
Collaborator Contribution Developing new array technologies and applying them to investigate interaction of Phosphatases
Impact n/a
Start Year 2012
 
Description Dr Elizabeth Farrant 
Organisation Cyclofluidic
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Private 
PI Contribution Knowledge transfer of single-cell technology. Design of custum built cell assay for dynamic single-cell resolution drug discovery.
Collaborator Contribution Provided resources in the form of reagents and drug compounds. Transfer of expertise in autonomous drug discovery.
Impact This collaborative research has resulted in further funding by a Knowledge Transfer Secondment of the EPSRC. The work is truly multidisciplinary where Cyclofluidic are a pharmaceutical company collaborating with our chemical biology and physical research team that is also engaged in engineering a new platform to integrate new cell assay technologies.
Start Year 2012
 
Description Dr Euan Stronach 
Organisation Imperial College London
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution MAC chip analysis of ovarian cancer cells, specifically the analysis of AKT and p53 protein copy number in single cells. Immunohistochemistry of ovarian cancer cells from Ascites fluid with several cancer markers.
Collaborator Contribution Provision of ovarian cancer cells from Ascites fluid. Provision of circulating cancer cells from patients with ovarian cancer.
Impact Further funding has been obtained for this collaboration in the form of a Pathway to Impact grant from the EPSRC.
Start Year 2013
 
Description Dr Jesper Glückstad 
Organisation Technical University of Denmark
Country Denmark, Kingdom of 
Sector Academic/University 
PI Contribution Erasmus-funded visit (proposal in progress); joint biophotonics research
Collaborator Contribution Optical probe development, instrument access, joint experiments
Impact Joint paper, new lines of enquiry
Start Year 2014
 
Description Dr Paul Donaldson 
Organisation Diamond Light Source
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution Imaging of nitrated human tissue comparing 2DIR and IR imaging.
Collaborator Contribution Provision of human tissue.
Impact n/a
Start Year 2014
 
Description Dr Stuart Bennett 
Organisation Aston University
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution MS identification of membrane proteins from Red blood cells.
Collaborator Contribution n/a
Impact n/a
Start Year 2013
 
Description Duncan Casey and Jayne Dooley at LMJU 
Organisation Liverpool John Moores University
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution Joint Paper
Collaborator Contribution Joint Paper
Impact Book Chapter which is in Publications section of ResearchFish
Start Year 2014
 
Description Jose Luis Garcia Gimerez 
Organisation University of Valencia
Country Spain, Kingdom of 
Sector Academic/University 
PI Contribution n/a
Collaborator Contribution Analysing oxidative modifications to Histone samples from 2 cell lines, one derived from young patients and one from old patients.
Impact n/a
Start Year 2012
 
Description Mendeley 
Organisation Mendeley: Free reference manager and PDF organizer
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Private 
PI Contribution Substantial consultation and development work undertaken. Work has included direct product development, collating user feedback, assistance with their institutional edition and liaison with information management staff. Helping specify and adapt their institutional edition package with Swets and Library staff
Collaborator Contribution Free access to Mendeley Ltd, a software company selling collaborative reference management, software ideal for large, multidisciplinary groups.
Impact This collaboration has given the research group a great opportunity to easily store and share publications related to our collaborative research. Papers and relevant reviews are easily shared and discussed online.
Start Year 2009
 
Description Prof Chris Braddock 
Organisation Imperial College London
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution Identify potential activators or inhibitors of SHP2 phosphatase by screening a library of compounds synthesized in Chris Braddock's lab.
Collaborator Contribution Provided a library of compounds synthesized in Chris Braddock's lab.
Impact n/a
Start Year 2013
 
Description Prof Malcom Jackson 
Organisation University of Liverpool
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution Mass spec analysis of young and aged mice muscle cells.
Collaborator Contribution Provided young and aged mice muscle for mass spec analysis.
Impact n/a
Start Year 2013
 
Description Prof Michael Stumpf 
Organisation Imperial College London
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution Single cell distributions showing the heterogeneous action of the anti-cancer agent Actinomycin D.
Collaborator Contribution Stochastic Simulations were performed using the provided data. Approximate Bayesian Computation was used to infer parameters which would make the distributions obtained from MAC chip data.
Impact n/a
Start Year 2014
 
Description Prof Michael Tisdale 
Organisation Aston University
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution Identifying a human Bif receptor using mass spectrometry
Collaborator Contribution n/a
Impact n/a
Start Year 2013
 
Description Prof Paul Winyard 
Organisation University of Exeter
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution MS analysis of nitrated BSA and HAS
Collaborator Contribution n/a
Impact n/a
Start Year 2013
 
Description Prof Sunil Shaunak 
Organisation Imperial College London
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution FTIR studies of dendrimer-based drugs.
Collaborator Contribution Provision of dendrimer-based drugs.
Impact n/a
Start Year 2013
 
Description Prof Tony Ng 
Organisation King's College London
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution n/a
Collaborator Contribution Investigating protein-protein interactions involving the SHP2 phosphatase under oxidative stress conditions.
Impact n/a
Start Year 2013
 
Description Professor Andy Pitt 
Organisation Aston University
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution Professor Andy Pitt is a collaborator and co-I on the grant 'Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and Wellbeing'.
Start Year 2011
 
Description Professor Jon Cooper 
Organisation University of Glasgow
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution Professor Jon Cooper is a collaborator and co-I on the grant 'Next Generation Analytical Tools: Application to Protein Oxidations that affect Human Health and Wellbeing'.
Start Year 2011
 
Description Professor Michael Stumpf 
Organisation Imperial College London
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution Professor Michael Stumpf is a collaborator on the grant 'Cellular Information Processing and Decision Making from Noise to Robust Phenotypes'.
Start Year 2011
 
Description Sir Steve Bloom 
Organisation Imperial College London
Country United Kingdom of Great Britain & Northern Ireland (UK) 
Sector Academic/University 
PI Contribution 2DIR measurements on drug binding.
Collaborator Contribution Providing drugs for 2DIR measurements.
Impact n/a
Start Year 2013
 
Title DEVICE AND METHOD FOR CAUSING AGGREGATION OF BLOOD CELLS AND METHOD OF TREATING BLEEDING FROM AN INJURY 
Description A device is disclosed for causing aggregation of blood cells to affect one or more fluid properties of blood. The device comprises: a surface acoustic wave (SAW) propagation surface configured to be brought into acoustic contact with blood; and a transducer arranged to provide SAWs on the SAW propagation surface that when coupled with blood in acoustic contact with the SAW propagation surface cause aggregation of blood cells in the blood to affect one or more fluid properties of the blood. Also disclosed are a method of causing aggregation of blood cells to affect one or more fluid properties of blood and a method of treating bleeding from an injury. 
IP Reference WO2015033139 
Protection Patent granted
Year Protection Granted 2015
Licensed No
Impact background for spin out
 
Title Fluidics apparatus for surface acoustic wave manipulation of fluid samples, use of fluidics apparatus and process for the manufacture of fluidics apparatus 
Description A fluidics apparatus for manipulation of at least one fluid sample is disclosed. A manipulation surface locates the fluid sample. A surface acoustic wave (SAW) generation material layer is provided. This is a polycrystalline material, textured polycrystalline material, biaxially textured polycrystalline material, microcrystalline material, nanocrystalline material, amorphous material or composite material. A transducer electrode structure arranged at the SAW generation material layer provides SAWs at the manipulation surface for interaction with the fluid sample. The manipulation surface has a phononic structure, for affecting the transmission, distribution and/or behaviour of SAWs at the manipulation surface. The apparatus is typically manufactured by reel-to-reel processes, to reduce the unit cost to a level at which the apparatus can be considered to be disposable after a single use. 
IP Reference CN103492078 
Protection Patent granted
Year Protection Granted 2014
Licensed No
Impact Background IP for spin out
 
Title SAMPLE NEBULIZATION 
Description A device for generating a nebulized sample for detection of an analyte. The device includes a surface acoustic wave (SAW) transducer and a superstrate. The superstrate has a first surface for coupling with the SAW transducer and a second surface for receiving a fluid sample incorporating the analyte. The fluid sample is nebulized from the second surface. The superstrate is provided with an electrical connection extending from the second surface of the superstrate to provide a conducting path from a charge source to the second surface of the superstrate. The charge source may be the surface of the transducer or an external voltage source. 
IP Reference US2014083174 
Protection Patent granted
Year Protection Granted 2014
Licensed No
Impact Enables leverage with other companies
 
Company Name SAW Dx 
Description "SAW Dx is an emerging point of care diagnostic (POC) device design and manufacturing venture focused on exploiting Acoustic-Flow technology based on the use of surface acoustic waves technology developed in the laboratory of Prof Jon Cooper, University of Glasgow. The Acoustic-Flow technology utilises the interaction of Surface Acoustic Waves (SAW) with a patient sample to drive a diagnostic test. The interactions are controlled by microstructures patterned on the surface of disposable chips, to shape sound in particular ways in a frequency dependant manner. Functions possible include fluid movement, mixing, particle patterning, enrichment, opening cells to release DNA and temperature cycling of a sample. Acoustic-Flow technology means that samples can be processed on-chip (no preprocessing required) and complex micro-fluidics are avoided. The SAW Dx business model is based on providing product and technology solutions to life sciences and diagnostics companies seeking to enhance their diagnostic product offerings through innovative point of care use. " 
Year Established 2013 
Impact SAW Dx is involved in 3 innovateUK projects, partnering with clinicians and manufacturing companies, focussed on developing point-of-care diagnostics for TB, animal health, and sexually transmitted diseases
 
Description 'FedEx delivery for single cells' - Using HOT to develop the world's most accurate single cell delivery system, Joint CDT conference, Warwick University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Student led conference at the University of Warwick. The speaker, Douglas Wylie, received the second place prize for student talk at this event.

n/a
Year(s) Of Engagement Activity 2014
 
Description Biological strategies for the investigation of proteomes, Research in progress session, Aston University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description British Society of Rheology midwinter meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Talk delivered at the British Society of Rheology midwinter meeting on Microfluidics and ultrasonics.
Year(s) Of Engagement Activity 2015
 
Description British Society of Rheology midwinter meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other audiences
Results and Impact Talk delivered at the British Society of Rheology midwinter meeting on Microfluidics and ultrasonics.
Year(s) Of Engagement Activity 2015
 
Description Clinical Needs and Sensing Solutions brainstorming session 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact This talk was a brainstorming session that involved discussions about how to integrate developed tools and technologies in societal, mainly healthcare, needs.

Possible collaborations were discussed.
Year(s) Of Engagement Activity 2012
 
Description Optical Manipulation of Chemically Functionalised Emulsions and Suspensions for Single Cell Analysis, OSA Optics in Life Sciences Conference, Hawaii 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Technologies to study protein oxidation in ageing. Life and Health Sciences Research day, Aston University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Two-Dimensional Infrared Spectroscopy as a Potential Tool for Tissue Imaging, SPEC 2014: Shedding New Light on Disease, Krakow, Poland 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description A doctrine of doctors 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description A first step towards practical single cell proteomics: a microfluidic antibody capture chip with TIRF detection, Brompton Respiratory BRU meeting, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Acoustic Microfluidics - What a sound idea 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Acoustofluidics 2014, Monash University Prato Centre, Italy 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Adrenalin-fuelled creativity 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Media (as a channel to the public)
Results and Impact Article stimulated thinking about possibilites academia.

n/a
Year(s) Of Engagement Activity 2012
 
Description Advances in Chromatography article for Chromatography Today magazine 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact Magazine helped promote research

N/A
Year(s) Of Engagement Activity 2015
 
Description Advantages and Limitations of Mass Spectrometry as a Diagnostic Toolfor Protein Oxidation in Redox Biology, SFRBM conference, Seattle 2014 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Best practice shared


Teach young researchers best practice
Year(s) Of Engagement Activity 2014
URL http://www.sfrbm.org/
 
Description Althea-Imperial Programme 2014/15 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Discussions and actions culminated in the department getting the Athena Swan Gold award.

n/a
Year(s) Of Engagement Activity 2014
 
Description Analysis of PTEN protein-protein interactions in altered redox conditions. Research in progress seminar, Aston University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Analysis of PTEN protein-protein interactions in its reduced and oxidized form by GSH affinity enrichment and MS-based label free protein quantitation, SFRRI Meeting, Kyoto, Japan 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Analysis of PTEN protein-protein interactions in its reduced and oxidized form by GSH affinity enrichment and MS-based label free protein quantitation. Postgraduate Research day, Aston University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Biochemistry of Redox Reactions. SFRR-Europe Summer School 2012 on Dietary factors and redox signalling, Spetses, Greece 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Biomolecule Oxidation: Biological Effects and Biomarkers 2015 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact UK-Denmark interactions started


Potential for collaboration discussed
Year(s) Of Engagement Activity 2015
 
Description COST CM1001 Workshop on Mass Spectrometry Analysis of Protein Oxidation 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact This workshop was very interesting and was very successful in generating new ideas and disseminating information.

New contacts were made for possible future collaborations.
Year(s) Of Engagement Activity 2013
 
Description CREST Award (British Science Association) 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Schools
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Café Scientifique Liverpool 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Public talk as part of the British Science Association's Café Scientifique programme on interdisciplinary research and biophotonics


N/A
Year(s) Of Engagement Activity 2015
URL http://www.cafescientifique.org/index.php?option=com_contentbuilder&title=uk-liverpool&controller=de...
 
Description Capillary zone electrophoresis coupled with an immunoassay for protein identification, Institute of Chemical Biology MRes Conference, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Student research talk performed at the end of the masters year to the entire cohort.

n/a
Year(s) Of Engagement Activity 2012
 
Description Challenges and successes in analysing adducts between proteins and phospholipid oxidation products, COST CM1001 Workshop, The Hebrew University, 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Characterisation of tyrosine nitration by two-dimensional infrared spectroscopy, 204th Infrared and Raman Discussion Group (IRDG) Meeting , University College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Characterisation of tyrosine nitration by two-dimensional infrared spectroscopy, Chemistry PostGrad symposium, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Chemical tools to probe phosphoinositide dependent signalling, Membrane, Morphology and Function, Fara San Martino, Abruzzo, Italy 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Continuous Ultrasonic Nebulisation Transfer for Mass Spectrometry using a Phononic Crystal Superstrate, British Mass Spectroscopy Society annual meeting 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Dara O'Briain's Science Club 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? Yes
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact Scientific Advice given to the TV show that has resulted in scientifically correct experiments and information being broadcasted on the BBC.

n/a
Year(s) Of Engagement Activity 2012
 
Description Design, Synthesis and Application of Chemical Biology Probes for Measuring Oxidative Protein Damage, Joint CDT conference, Warwick University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Talk at a student led conference at the University of Warwick

n/a
Year(s) Of Engagement Activity 2014
 
Description Design, synthesis and activity profiling of modified 3-and 5-hydroxypicolinic acid vanadyl complexes against PTEN as models for targeted probes, Institute of Chemical Biology MRes Conference, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Student research talk performed at the end of the masters year to the entire cohort.

n/a
Year(s) Of Engagement Activity 2012
 
Description Design, synthesis and biological evaluation of fluorescent vanadyl complexes - towards the development of targeted probes for monitoring oxidative damage, Chemistry PostGrad symposium 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Detection and quantification of protein oxidation in sarcopenic models: a mass spectrometry study, LHS Postgraduate Research Day, Aston University, UK, 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Detection and quantification of protein oxidation in sarcopenic models: a mass spectrometry study, LHS Postgraduate Research Day, Aston University, UK, 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Detection and quantification of protein oxidation in sarcopenic models: a mass spectrometry study, Life beyond the PhD, Cumberland lodge, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Detection and quantification of protein oxidation in sarcopenic models: a mass spectrometry study, Life beyond the PhD, Cumberland lodge, UK 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Detection and quantification of protein oxidation in sarcopenic models: a mass spectrometry study. SFRR-E meeting 2014, Campus des Cordeliers, Paris 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Developing Diagnostics for the Developing World, SPIE Photonics West, San Francisco 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description EVV 2DIR Spectroscopy for the detection and analysis of Biomolecular complexes, geometry/structure determination and analytical studies of proteins and metabolites, School of Chemistry, University of Nottingham 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Electron-Vibration-Vibration coupling as readout signature for biomolecular composition, structure and dynamics, Joint Meeting of the British and German Biophysical Society, Hünfeld, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description FameLab masterclass 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Talk was received very well and many discussions were held afterwards.


n/a
Year(s) Of Engagement Activity 2013
 
Description FedEx delivery for single cells using 'HOT' - Holographic Optical Trapping as a tool for quantifiable cell biology, Chemistry PostGrad symposium, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Glasgow Explorathon 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Stand and presentations were received very well and many questions and thoughts were shared with our scientific outreach team.

n/a
Year(s) Of Engagement Activity 2014
 
Description Glasgow Science Centre 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact The table top presentations sparked interest in the public and we received many questions about our research.

The Glasgow Science Centre loved our presentation and is looking forward to host our outreach activities again.
Year(s) Of Engagement Activity 2014
 
Description Harnessing mass spectrometry to investigate oxidative modifications of proteins. STRESS IN HEALTH AND DISEASE IV SPANISH AND PORTUGUESE MEETING ON FREE RADICALS VALENCIA 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Imperial Fringe 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Talk sparked interest and got advice for further outreach event presentations.


n/a
Year(s) Of Engagement Activity 2013
 
Description Interfacing surface acoustic wave nebulisation with LC-MS 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact good feed back


Informed international audience of SAWN LC/MS
Year(s) Of Engagement Activity 2015
 
Description International Conference on Ultrasonics 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact Talk delivered by PhD student Ben Tiller at the annual congress on ultrasonics in Metz France. Discussed current research with a variety of academics.
Year(s) Of Engagement Activity 2015
 
Description Interview with Andy Roast 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Media (as a channel to the public)
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Invited Talk at 8th International Conference on Advanced Vibrational Spectroscopy (ICAVS8), 12-17 July 2015, Vienna, Austria 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact Scientific results were presented to a large audience and this led to interesting questions and discussions
Increased the awareness of the EVV 2DIR technique and the important scientific challenges to which it can be applied
Year(s) Of Engagement Activity 2015
 
Description Lipid oxidation products: signalling and biomarkers for inflammatory disease. Oxygen Club of California World Congress 2012 on OXIDANTS AND ANTIOXIDANTS IN BIOLOGY: CELL SIGNALING AND NUTRIENT - GENE INTERACTIONS, 20-23 June 2012, Alba, Italy 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description London Sci-Fi Convention 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact The stand at the SciFi Convention received a lot of interest from pupils to older people. People were fascinated by the presented science and the possibilities it offers.

Conference organiser thought our outreach stand and table-top demos were one of the highlights of the convention and looks forward to having more scientific attendees/presenters in future events.
Year(s) Of Engagement Activity 2014
 
Description Magnetide-doped PDMS as a solid phase support for phosphopeptides 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Informed international audience of the development of a new enrichment strategy

Very good feed back
Year(s) Of Engagement Activity 2014
 
Description Mapping oxidative modifications of PTEN, SFRRI International Meeting, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Mass Spectrometry Analysis of Protein Oxidation: Basic Principles and Application to Neutrophil Extracellular Traps. Birmingham Dental School, 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Mass spectrometry analysis of modified bee venom phospholipase A2 in correlation with increased activity, SFRRI International Meeting, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Mass spectrometry and proteomic approaches to identity oxidative protein modifications, Aston University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Mass spectrometry and proteomic approaches to identity oxidative protein modifications, COST CM1001 Workshop, The Hebrew University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Mass-spectrometry as a diagnostic platform for oxidative modification of proteins. Life and Health Sciences Research day, Aston University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Metals in medicine: Diagnostic and therapeutic agents 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Presentation was very successful and culminated in many questions asked.


n/a
Year(s) Of Engagement Activity 2013
 
Description Microfluidic Antibody Capture Chips: Battling Cancer One Cell at a Time, Chemistry PostGrad symposium, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Microfluidic Antibody Capture Chips: Battling Cancer One Cell at a Time, Joint CDT Conference, Warwick University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Talk at a student led conference at the University of Warwick. The speaker, Michael Barclay, won the first place talk prize at this event

n/a
Year(s) Of Engagement Activity 2014
 
Description Microfluidic-based single molecule counting approaches to diagnostics, Novartis external seminar program, Novartis, Basel, 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Microfluidics and Miniaturised Assays for Single Cell Analysis: Applications to Basic Biology and Clinical Challenges, Royal Society of Chemistry Analytical Research Forum, London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Miniaturisation of Technologies Measurement and Impact on Healthcare 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact The talk finished with interesting audience questions and further discussions were led at the end of the event.


Possibilities for collaborative work were discussed after the meeting.
Year(s) Of Engagement Activity 2012
 
Description More than just 'stinks': Using chemical tools to unlock biology' 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact Talk stimulated thinking in 6th form students who were excited to see the possibilities in scientific research.

n/a
Year(s) Of Engagement Activity 2012
 
Description Multidisciplinary research colloquium 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Participants in your research and patient groups
Results and Impact Organised event; gave final talk


New collaborations developed; resource and idea sharing
Year(s) Of Engagement Activity 2015
 
Description Multiplexed Infectious Disease Testing - Sound Point-of-Care Diagnostics, Lab-on-a-Chip European Congress, Berlin, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Networking and Collaborations 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact The talk finished with interesting audience questions and further discussions were led at the end of the event.


Talk sparked interest and got advice for further outreach event presentations.
Year(s) Of Engagement Activity 2013
 
Description New Vanadium Complexes as Optical Probes to Detect Cys Sulfenic Modifications in PTEN, EuroBIC 12, Zurich, Switzerland 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Novel Micro-delivery systems: Through live single cell membranes and beyond, Institute of Chemical Biology MRes Conference, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Student research talk performed at the end of the masters year to the entire cohort.

n/a
Year(s) Of Engagement Activity 2012
 
Description Novel Tools and Technologies for Single Cell Proteomics Analysis, Single Cell Analysis Conference, Cambridge, Massachusetts, USA, 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Novel nanodelivery systems to single cell membranes and beyond, Photon12, Durham 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Novel nanodelivery systems to single cell membranes and beyond, SFRRI International Meeting, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Oxidation of proteins: mechanisms and role in signalling, methods for analysis of protein oxidation & Oxidative Stress in inflammation and atherosclerosis 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact These lecture series were very well received.

n/a
Year(s) Of Engagement Activity 2013
 
Description Oxidized phospholipids in the vascular system and their cellular effects, X Meeting of the Spanish Group for Research on Free Radicals (Geirli) Symposium on oxidative stress and redox signalling in biology and medicine, Aula Magna, Faculty of Medicine, 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Oxidized proteins in chronic disease: how to detect them and why they are important, ICA-Symposium Summer 2014, Salzburg 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description PH-domain mimetics: potential drugs for Lowe Syndrome?, 4th international Symposium of the Lowe syndrome Trust - Molecular and clinical advances in Lowe Syndrome, Royal Society London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description PRINCE2 Project Management Overview Training Course 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Dr Julia Davies gained an understanding of project management and would like to pursue this as a career


Dr Julia Davies will enrol on a PRINCE2 training course and gain qualifications
Year(s) Of Engagement Activity 2015
 
Description Pleckstrin homology domain mimicry: chemical biology of phosphoinositide signalling, VIPCA conference on Chemical Biology: Methods and Progress, Vienna University, Austria 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Poster Presentation at IRDG Xmas Meeting 2014 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Poster was well received and initiated interesting discussions


I have been asked to give a talk at the IRDG Xmas Meeting 2015
Year(s) Of Engagement Activity 2014
 
Description Poster presented at HUPO2014. Title: The Redox Interactome of PTEN: Cellular and Protein Specific Changes 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Presented research methods and findings


Positive feedback from other academics and stimulating discussion
Year(s) Of Engagement Activity 2014
 
Description Programmable light 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact The talk finished with interesting audience questions

n/a
Year(s) Of Engagement Activity 2012
 
Description Progress Towards Point of Care Methods for Single Cell Protein Analysis, 2nd Annual Single Cell Genomics & Transcriptomics Asia Congress 2014, Singapore 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Progress in Deconvolving Structural Elements In a Small Protein Using EVV 2DIR. 6th International Conference on Coherent Multidimensional Spectroscopy 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Project Management 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact Talk was received very well and many discussions were held afterwards.


n/a
Year(s) Of Engagement Activity 2013
 
Description Protein Oxidative Modifications: importance in pathophysiology and measurement. Proteomics Workshop 2012 Aveiro, Portugal 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Protein modification and phospholipid oxidation. Society for Free Radical Research Europe 2013 Conference Athens 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Protein oxidation in stress and disease - finding the needle in the haystack. Analytical methods to study oxidative damage, antioxidants & drugs (CONFERENCE). Medical University of Bialystok 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2011
 
Description Protein oxidation in stress and disease - finding the needle in the haystack. Society for Free Radical Research Europe 2011 Conference Istanbul 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2011
 
Description Proteome-on-a-chip: Automation of Protein Separation, Deposition, and Readout of Oxidative Damage, Joint CDT Multidisciplinary conference, Royal Academy of Engineering 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Proteomic profiling of oxidations of muscle proteins in disease, Research in Progress Seminar, Aston University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Quantitative Single Cell and Single Molecule Proteomics for Clinical Studies, Cambridge Healthtech Institute's Second Annual Quantitative Digital Detection Technologies conference, Boston 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Quantitative Single Cell and Single Molecule Proteomics for Clinical Studies. Institute of Virology, Kyoto University, Japan 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description RSC Chemistry World A Doctrine of Doctors 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Research in Progress seminar, Aston University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Royal Society Summer Science Exhibition 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Public/other audiences
Results and Impact Annual event that takes place at the Royal Society in London. Around 20 groups from around the UK and beyond are selected to take part in this prestigious event. The event involved constructing a stand and a series of interactive demos. This was split across the three institutions, Imperial, Glasgow and Aston with each institution creating one demo or activity.

The event spanned an entire week which involved interacting with secondary school students, members of the general public and fellows of the Royal Society.

Since the event we have a number of requests for more detailed information which related to our stand. This included an A-Level student asking for information about oxidative damage for a school presentation and a student at the Royal College of Art asking for information about how sound waves can be used to interact with biological material.
Year(s) Of Engagement Activity 2015
URL http://sse.royalsociety.org/2015
 
Description Salter's Chemistry Challenge 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Schools
Results and Impact Presentation was very successful and culminated in many questions asked.


Pupils were very excited by the talk and asked many intriguing questions.
Year(s) Of Engagement Activity 2013
 
Description Sarcoplasmic reticulum protein as models of protein oxidation in ageing. Life and Health Sciences Research day, Aston University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Sarcoplasmic reticulum protein as models of protein oxidation in ageing. Research in Progress seminar, Aston University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description SciBar Liverpool 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Public/other audiences
Results and Impact Proxomics member took part in a meet up in a bar in Liverpool that occurs monthly. Around 50 people usually attend to listen to talks about various scientific topics. The event got regular members of the public and students interested and engaged with biophotonics.
Year(s) Of Engagement Activity 2015
URL http://www.cafescientifique.org/index.php?option=com_contentbuilder&title=uk-liverpool-scibar&contro...
 
Description Sense About Science 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Sense About Science Peer Review Workshop 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Public/other audiences
Results and Impact Discussions led to improve outreach program.

n/a
Year(s) Of Engagement Activity 2013
 
Description Single Cell Analysis of Proteins from Clinical Samples Relevant to Human Health, HUPO Conference, Yokohama, Japan 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Single Cell Analysis: A new frontier for Clinical Research and Clinical Practise; Measuring protein copy number and variation in a single cell, High Throughput/High Content Technologies Community, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Single Cell Distributions: Investigating the effect of Actinomycin D on p53 at the single cell level with the use of Microfluidic Antibody Capture (MAC) devices, Institute of Chemical Biology MRes Conference, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Student research talk performed at the end of the masters year to the entire cohort.

n/a
Year(s) Of Engagement Activity 2012
 
Description Single Cell Protein Analysis from Clinical Samples Relevant to Human Health, Single Cell Genomics and Transcriptomics Asia Congress, Singapore 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Single Cell Protein Analysis: Potential for impact on clinical research and clinical outcomes, ITMAT 9th Annual International Symposium, Perelman School of Medicine, University of Pennsylvania 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Single Cell Proteomics: Understanding Cancer One Cell at a Time, Point-of-Care Diagnostics workshop, 4 December 2012, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Single Cell Proteomics: Understanding Cancer One Cell at a Time, SET for Britain 2013 (final), House of Commons, Westminster 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/parliamentarians
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Small-Molecule Optical Probes for Fluorescence Imaging of Inflammation through FPRs. William Harvey Research Institute (WHRI), Queen Mary University, London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Smart Droplet Microtools: Probing a single cell using "optical tweezers", SET for Britain 2012, Terrace Marquee, Houses of Parliament 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Policymakers/parliamentarians
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Spectroscopy in a suitcase 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description St. Edward's Catholic Primary School Summer Fair 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Schools
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Study of protein-tyrosine nitration by two-dimensional infrared (2DIR) spectroscopy, Institute of Chemical Biology MRes Conference, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact Student research talk performed at the end of the masters year to the entire cohort.

n/a
Year(s) Of Engagement Activity 2012
 
Description Study of protein-tyrosine nitration by two-dimensional infrared vibrational spectroscopy, SFRRI International Meeting, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach National
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Surface Acoustic Wave Nebulisation on Structured Superstrates, Single Cell Analysis Europe Conference, Berlin, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Symposium at SFRRE 2014 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Teams from US and Europe brought together


New collaborations established
Year(s) Of Engagement Activity 2014
URL https://www.sfrr-europe.org/
 
Description Talk at Imperial Science Foresight 2035 Conference 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Invited talk by PhD student within the group to discuss their research and project the outcome of the work 30 years into the future.
Year(s) Of Engagement Activity 2015
 
Description Targetted mass spectrometry methods for detecting oxPTMs relevant to ageing and epigenetics. 5th International Symposium on Nutrition, Oxygen Biology & Medicine, UPMC Campus des Cordeliers, Paris 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Targetted mass spectrometry methods for detecting oxPTMs. SFRR-E meeting 2014, Campus des Cordeliers, Paris 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Targetted mass spectrometry methods for detecting oxPTMs., HUPO 5-8 October 2014, Madrid 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Technologies to study protein oxidation in ageing. Postgraduate Research day, Aston University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Local
Primary Audience Postgraduate students
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description The Why, How and What of Single Celll Analysis: Towards Routine Analysis of Human Tissue Relevant to Human Health, Conway Institute, University College Dublin, Dublin, Ireland 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description The proteomics of oxidation: optical tools to probe the microscale, Brompton Respiratory BRU meeting, Imperial College London 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description The road ahead: from idea to implementation 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach National
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description ThinkTank 'meet the expert' 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach Regional
Primary Audience Public/other audiences
Results and Impact Inspired young children to be interest in Science


Very good feed back from ThinkTank
Year(s) Of Engagement Activity 2015
URL http://www.birminghammuseums.org.uk/thinktank/whats-on/meet-the-experts
 
Description Towards Practical Single Cell Proteomics: A Microfluidic Antibody Capture Chip with TIRF Detection, SFRRI International Meeting, Imperial College London 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach Regional
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2012
 
Description Towards Single Cell Analysis of Proteins from Clinical Samples Relevant to Human Health, Single Cell Analysis Europe Conference, Berlin, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Using 2DIR spectroscopy to probe tyrosine nitration, Joint CDT Conference, Warwick University 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach Regional
Primary Audience Postgraduate students
Results and Impact Talk at a student led conference at the University of Warwick

n/a
Year(s) Of Engagement Activity 2014
 
Description Using Phononic Crystals to Shape Complex Fluid Flows, Lab-on-a-Chip European Congress, Barcelona, Spain 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation keynote/invited speaker
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2013
 
Description Using microfluidics to monitor oxidative stress response on a single cell level, Microfluidics 2014, Heidelberg, Germany 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description Workshop at SFRBM 
Form Of Engagement Activity A formal working group, expert panel or dialogue
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other academic audiences (collaborators, peers etc.)
Results and Impact Best practice shared


Taught young researchers best practice
Year(s) Of Engagement Activity 2014
URL http://www.sfrbm.org/
 
Description mart Droplet Microtools: an optically controlled platform for single- and sub-cellular surgery, International Biophysics Congress 2014, Brisbane, Australia 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Type Of Presentation poster presentation
Geographic Reach International
Primary Audience Professional Practitioners
Results and Impact n/a
Year(s) Of Engagement Activity 2014
 
Description £4.6m project aims to understand causes of ageing 
Form Of Engagement Activity A magazine, newsletter or online publication
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Media (as a channel to the public)
Results and Impact Local interest raised in the project.

n/a
Year(s) Of Engagement Activity 2012