Synthesis and Characterisation of Dual Functional Nanoparticles for Probing the Role of Reactive Oxygen Species in Cellular Function
Lead Research Organisation:
University of Hull
Department Name: Physical Sciences
Abstract
The proposal concerns the synthesis and characterisation of dual functional nanoparticles for studying the response of cells to Reactive Oxygen Species (ROS). ROS have been implicated in ageing, heart disease, and recently, in the onset of Alzheimer's disease. The nanoparticles will contain a dye which responds to changes in calcium ions. Calcium ions are an important species which the cell uses to send messages from one site to another. Our dual functional nanoparticles will have the ability to sense changes in calcium ions in their immediate environment within the cell, but what differentiates them from other similar species is the ability to generate bursts of ROS when excited with light. The ROS generating component will be attached to the nanoparticles after they are formed and are composed of molecules called porphyrins. The ability to generate ROS by porphyrins is currently exploited in the Photodynamic Therapy of diseases such as cancer and age related macular degeneration. By changing the chemical structure of the porphyrins and then attaching them to the nanoparaticle we also plan to cause them to accumulate at important structures within the cells, such as the mitochondria. Currently, no similar tools are available for studying ROS in cells and it is anticipated that the successful development of our system will generate important new data relating to how cells respond to ROS.
People |
ORCID iD |
Ross Boyle (Principal Investigator) |
Publications
Ruiz-González R
(2017)
NanoSOSG: A Nanostructured Fluorescent Probe for the Detection of Intracellular Singlet Oxygen
in Angewandte Chemie
Ruiz-González R
(2017)
NanoSOSG: A Nanostructured Fluorescent Probe for the Detection of Intracellular Singlet Oxygen
in Angewandte Chemie International Edition
Giuntini F
(2014)
Huisgen-based conjugation of water-soluble porphyrins to deprotected sugars: towards mild strategies for the labelling of glycans.
in Organic & biomolecular chemistry
Giuntini F
(2011)
Synthetic approaches for the conjugation of porphyrins and related macrocycles to peptides and proteins.
in Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology
Giuntini F
(2014)
Conjugatable water-soluble Pt(ii) and Pd(ii) porphyrin complexes: novel nano- and molecular probes for optical oxygen tension measurement in tissue engineering
in Photochemical & Photobiological Sciences
Giuntini F
(2012)
Orthogonally bifunctionalised polyacrylamide nanoparticles: a support for the assembly of multifunctional nanodevices.
in Nanoscale
Description | Development of novel probes for oxidative stress |
First Year Of Impact | 2012 |
Sector | Healthcare,Pharmaceuticals and Medical Biotechnology |
Impact Types | Societal |
Description | Dr A. MacRobert |
Organisation | University College London |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Collaboration with University College London |
Start Year | 2012 |
Description | Prof. Beate Roder |
Organisation | Humboldt University of Berlin |
Country | Germany |
Sector | Academic/University |
PI Contribution | Collaboration |
Start Year | 2012 |
Description | Prof. Nonell Santi |
Organisation | University of Barcelona |
Country | Spain |
Sector | Academic/University |
PI Contribution | Collaboration with University of Barcelona |
Start Year | 2012 |
Description | Health Research Board Ireland |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Primary Audience | Participants in your research or patient groups |
Results and Impact | Panel Membership . Awarding Body - Health Research Board Ireland, Name of Scheme - Panel Membership |
Year(s) Of Engagement Activity | 2011 |
Description | Membership Workgroup and Website Workgroup |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Primary Audience | Participants in your research or patient groups |
Results and Impact | Invited Membership . Awarding Body - European Society for Photobiology, Name of Scheme - Committee membership |
Year(s) Of Engagement Activity | 2012 |
Description | Science Foundation Ireland |
Form Of Engagement Activity | A formal working group, expert panel or dialogue |
Part Of Official Scheme? | No |
Primary Audience | Participants in your research or patient groups |
Results and Impact | Panel Membership . Awarding Body - Science Foundation Ireland, Name of Scheme - Panel Membership |
Year(s) Of Engagement Activity | 2012 |