Development of a system for simultaneous multiplane image acquisition for live cell
Lead Research Organisation:
University of Kent
Department Name: Sch of Biosciences
Abstract
During this project the student will develop a system to allow truly synchronous multi-plane image acquisition, which will significantly improve the time-resolution during 3d-live-cell imaging. Cairn have achieved successful simultaneous imaging at two different depths into a sample. The student will develop and extend this technique using Cairn's novel Multisplit device, which allows an image to be split into 4 equivalent images on the same camera chip. The student will integrate lenses into this apparatus to obtain different focal lengths to image 4 discrete z-planes on the chip. They will then develop this method and use a combination of piezo and light path splitters to optimize control and stability of spatial distribution between images to permit z distances to be defined by the user, according to experimental needs.
The aims for the student are to:
1. Develop a simultaneous 4-plane image acquisition system with the aim of making the method widely available.
2. Make a detailed high temporal resolution multi-dimension study of rapid movements of myosins and dynamic rearrangements of the actin cytoskeleton to further our understanding of their regulation and function
The aims for the student are to:
1. Develop a simultaneous 4-plane image acquisition system with the aim of making the method widely available.
2. Make a detailed high temporal resolution multi-dimension study of rapid movements of myosins and dynamic rearrangements of the actin cytoskeleton to further our understanding of their regulation and function
Organisations
Publications
Baker K
(2019)
TORC2-Gad8-dependent myosin phosphorylation modulates regulation by calcium.
in eLife
Brooker HR
(2018)
A novel live-cell imaging system reveals a reversible hydrostatic pressure impact on cell-cycle progression.
in Journal of cell science
Johnson CA
(2018)
Temperature sensitive point mutations in fission yeast tropomyosin have long range effects on the stability and function of the actin-tropomyosin copolymer.
in Biochemical and biophysical research communications
Studentship Projects
Project Reference | Relationship | Related To | Start | End | Student Name |
---|---|---|---|---|---|
BB/M015130/1 | 30/09/2015 | 30/03/2020 | |||
1666608 | Studentship | BB/M015130/1 | 30/09/2015 | 30/03/2020 |
Description | 1. Fluorescence imaging optimisation determined microscopy conditions to reduce the damaging effects of light energy on live cell samples. 2. Characterised regulatory sites in the myosin V motor protein, responsible for the transport of materials within living cells. The regulatory sites impact myosin V motility, localisation and its role in cytoskeletal assembly and cell growth cycle. 3. Characterised regulatory a site in the myosin I motor protein, responsible for the uptake of matter by living cells and cell cycle regulation. The regulatory site impacts associated proteins binding to myosin I and contributes to cell morphology maintenance and cell cycle regulation. Data gathered throughout the PhD was gathered via fluorescence imaging and genetic cloning techniques. |
Exploitation Route | 1. Light energy damage in live cells during fluorescence imaging may be reduced following the methodology used. 2-3. Data gathered about myosin I and myosin V may be used to further understand the multiple dynamic processes that occur within the cell as well as mutations that cause disruptions to normal function. |
Sectors | Other |