Engineering FUngal Networks for Ground Improvement (Engineering FUNGI)

Lead Research Organisation: University of Strathclyde
Department Name: Civil and Environmental Engineering

Abstract

Conventional ground improvement techniques are highly invasive, frequently energy intensive and may require the introduction of environmentally damaging chemicals or carbon-intensive materials into the subsurface (e.g. chemical grouts, cement). The construction sector is responsible for 7% of carbon emissions in the UK. The UK target for 80% reduction in carbon emissions by 2050 (against the 1990 baseline) presents both challenges and tremendous opportunities for the UK construction sector in the transition towards a low-carbon economy. The use of cementitious materials is pervasive in conventional ground improvement techniques, and with cement production contributing 5-7% of total global CO2 emissions there is a clear need for the development of new ground improvement technologies.

Over the last ten years the geotechnical engineering research community has witnessed the creation of a new subdiscipline: biogeotechnics a multi-disciplinary field at the interface of biology, geochemistry, soil mechanics, and geotechnical engineering. This represents a paradigm shift in geotechnical engineering- until now the accepted view has been to consider the ground as sterile and inert; now engineers are exploring the potential for use of biological and biochemical processes in ground engineering applications. This proposal represents the first steps towards the development of a novel low-carbon, minimal intervention, biologically based technology using engineered fungal networks.

Biological soil crusts in nature (consisting of fungi, bacteria and other organisms) are known to withstand erosion due to water or wind action. This project will investigate filamentous fungi, i.e. fungi which grow hyphae (tube-like structures). It is thought that fungal hyphae behave similar to plant roots - penetrating between soil particles and entangling them -helping to bind soil particles together, but on a smaller scale. Furthermore, fungi can secrete biochemical products, which may also contribute to binding of soil particles. This project will systematically quantify the mechanical benefit of fungal treatment in soils by investigating three different types of fungi and their ability to enhance the behaviour of different soil types. The project will determine the conditions required for rapid fungal network growth to occur and optimise the orientation of hyphal development to give maximum mechanical benefit. The dataset arising from the proposed experimental campaign will act as a springboard for the development of a new range of nature inspired ground improvement technologies.

The research proposed could transform how we consider the design of, development and deployment of ground improvement technologies. Rather than subject the ground to different energy intensive or invasive techniques, this research proposes to 'grow' the required level of treatment through the use of fungal networks. The process could be engineered using external stimuli to orient the hyphal networks as required for site-specific applications. This project will investigate the feasibility of the deployment of fungal networks as a ground improvement technology.

Planned Impact

This project takes a creative and ambitious approach to ground improvement technologies. The work carried out in this project will result in economic, societal and additional benefits:
1. Economy- It is envisaged that the results from this project will contribute to the emerging field of biogeotechnical engineering. Other biogeotechnical engineering processes have been brought from fundamental laboratory research to deployment on commercial projects in approx.10 years. Through this project and by exploring alternative biological processes and assessing their suitability for deployment in ground engineering, there is an opportunity for the UK ground engineering industry to take the lead in biogeotechnical engineering. It is anticipated that the dataset arising from the proposed experimental campaign will act as a springboard for the development of a new range of nature inspired ground improvement technologies. The development of new products and technologies in the long-term will contribute to the economic competitiveness of the UK and contribute towards the transition of the UK construction sector to low-carbon technologies.

2. Society- The project represents the first step towards the development of a new range of low-carbon ground improvement technologies, which could contribute towards the ultimate goal of developing geotechnical infrastructure more resilient to environmental change. In the long-term, technologies arising from the research carried out in this project could contribute indirectly to enhancing the quality of life for the public, for example via their deployment as flood embankment protection measures. The outcomes of this project are expected to lead to low-cost ground improvement technologies, which involve minimal transportation of materials to site (reducing environmental impacts during treatment), making them suitable for deployment in remote areas and in international development contexts.

3. People - In the short term the project will equip two researchers with skills in soil mechanics, geomicrobiology and geotechnics. Education/research experience in this multi-disciplinary area is necessary to ensure adequately skilled people can lead this research field in the future but also to ensure that arising innovations are deployed in engineering practice.

Publications

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Assadi-Langroudi A (2021) Recent Advances in Nature-Inspired Solutions for Ground Engineering (NiSE) in International Journal of Geosynthetics and Ground Engineering

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El Mountassir G (2018) Applications of Microbial Processes in Geotechnical Engineering. in Advances in applied microbiology

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Salifu E (2022) Hydraulic behaviour of fungal treated sand in Geomechanics for Energy and the Environment

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Salifu E (2021) Fungal-induced water repellency in sand in Géotechnique

 
Description In the Engineering FUNGI project we have demonstrated the following:
1. that fungal growth in soils is influenced by temperature, water content and the nature of nutrients supplied.
2. that fungal growth can induce water repellency in soils
3. that treatment of of soils with fungi can significantly reduce soil loss via erosion and that a period of greater than 1 week is necessary to enable adequate hyphal networks to develop.
4. that different fungal species have different growth behaviours (some prefer to grow at the soil-air boundary, whereas others prefer to grow within the soil) and that this results in different patterns of soil loss during erosion events.
5. supplementing fungal treatment with additional nutrients (carbon and nitrogen) further reduces soil erosion.
Exploitation Route The results of the Engineering FUNGI project show that fungal networks can induce changes in soil behaviour which could be beneficial in a range of applications. For example fungal treatment could be used by ground engineering contractors to create semi-permeable barriers to water infiltration that could be used to divert water on hillslopes, to provide caps above landfills, to aid in water harvesting, to divert water away from expansive soils. The increased resistance to erosion afforded by fungal treatment, suggests that this technology could have application in stabilising hillslopes, that are subject to recurrent shallow landslides, particularly those triggered by rainfall. The technology could also have application in reducing erosion in earthen flood embankments.
Sectors Construction,Environment

 
Description The research findings are generating interest among industry ground engineering practitioners. BAM Ritchies are interested in the potential of the fungal biotechnologies being developed. They are project partner on my Future Leaders Fellowship which builds upon the work conducted in Engineering FUNGI. MY FLF began in March 2022 and BAM Ritchies have committed in-kind support to the project and a commitment to support future field-trials of technologies developed.
First Year Of Impact 2020
Impact Types Societal

 
Description EPSRC Doctoral Training Partnership 2020-21 University of Strathclyde
Amount £35,680 (GBP)
Organisation Engineering and Physical Sciences Research Council (EPSRC) 
Sector Public
Country United Kingdom
Start 01/2021 
End 06/2024
 
Description Future Leaders Fellowship
Amount £1,289,476 (GBP)
Funding ID MR/V025376/1 
Organisation United Kingdom Research and Innovation 
Sector Public
Country United Kingdom
Start 03/2022 
End 03/2026
 
Title Data for: "Fungal-induced Water Repellency in Sand" 
Description Description of data presented in .csv files: Fig. 2: Evolution of contact angle with growth duration. Levels of water repellency are shown based on classification presented in Table 2. Fig 3: Influence of initial degree of saturation on water drop penetration time. Levels of water repellency are shown based on classification presented in Table 2. Fig. 5: Assessment of water repellency for treated soil specimens subjected to mixing every 48hrs to disrupt fungal growth up to 20 days: (a) Contact angle and (b) Water drop penetration time. The dashed vertical lines indicate days on which specimen. The dashed vertical lines indicate days on which specimens were mixed. The arrows show that the initially extreme WR exhibited by specimens (black symbols) was completely lost immediately after mixing on the 6th day. As contained in the manuscript and data headings: MEDT means Molarity of Ethanol Droplet Test SDM means Sessile Drop Method WDPT means Water Drop Penetration Time WR means Water Repellency 
Type Of Material Database/Collection of data 
Year Produced 2020 
Provided To Others? Yes  
URL https://pureportal.strath.ac.uk/en/datasets/1671ac61-507a-4fc9-a215-370c4178c8c5
 
Title Data for: "Hydraulic behaviour of fungal treated sand" 
Description Data underpinning paper "Hydraulic behaviour of fungal treated sand", Doi: 10.1016/j.gete.2021.100258 
Type Of Material Database/Collection of data 
Year Produced 2021 
Provided To Others? Yes  
Impact Underpinned publication of "Hydraulic behaviour of fungal treated sand", Doi: 10.1016/j.gete.2021.100258. 
URL https://pureportal.strath.ac.uk/en/datasets/data-for-hydraulic-behaviour-of-fungal-treated-sand
 
Title Data for: "Preliminary observations of the shear behaviour of fungal treated soil" 
Description Description of data presented in .csv files: Fig 3a presents the shear stress - horizontal displacement of treated (T) and untreated (U) sand under applied normal stresses of 25, 51 and 100kPa T means Treated specimen (i.e. with fungal growth present) U means Untreated specimen (i.e. control; no fungal hyphal growth present) T_25kPa means Treated specimen at normal stress of 25kPa; U_51kPa means Untreated specimen at normal stress of 51kPa; and so on. Fig 3b presents the volumetric behaviour of treated and untreated soils under applied normal stresses of 25, 51 and 100kPa Fig 3c presents the respective with peak and ultimate shear stress values along with the corresponding normal shear stresses from which the failure envelope of treated and untreated soil were derived. Fig. 4a presents the shear stress - horizontal displacement of treated (T) and untreated (U) specimens with varied amounts of lignocel. U_94S:6LIG means Untreated specimen composed of 94% sand and 6% lignocel T_94S:6LIG means Treated specimen composed of 94% sand and 6% lignocel U_70S:30LIG means Untreated specimen composed of 70% sand and 30% lignocel T_70S:30LIG means Treated specimen composed of 70% sand and 30% lignocel U_50S:50LIG means Untreated specimen composed of 50% sand and 50% lignocel T_50S:50LIG means Treated specimen composed of 50% sand and 50% lignocel Fig. 4b presents the volumetric behaviour of treated and untreated specimens with varied amounts of lignocel Fig. 5a presents the shear stress - horizontal displacement of specimens treated (T) and untreated (U) with homogenate and tested immediately after mixing (day 0) and 1 month after. UFH_94S:6LIG means Untreated with Fungal Homogenates and composed of 94% sand and 6% lignocel TFH_94S:6LIG (Day 0) means Treated with Fungal Homogenates; composed of 94% sand and 6% lignocel; tested immediately after mixing TFH_94S:6LIG (1 month) means Treated with Fungal Homogenates; composed of 94% sand and 6% lignocel; tested after 1 month of incubation/fungal growth. Fig. 5b presents the volumetric behaviour of specimens treated (T) and untreated (U) with homogenate and tested immediately after mixing (day 0) and 1 month after Data embargo until 29/06/19 
Type Of Material Database/Collection of data 
Year Produced 2019 
Provided To Others? Yes  
Impact The first dataset presenting shear stress behaviour of fungal treated soils. 
URL https://pureportal.strath.ac.uk/en/datasets/33cf0fc5-7e8d-4240-a931-b1b669231bc9
 
Description Collaboration with Università di Napoli 
Organisation University of Naples
Country Italy 
Sector Academic/University 
PI Contribution One of the researchers working on the project was seconded to Università di Napoli. He collaborated with colleagues from the Department of Civil Engineering, Department of Earth Science Environment and Resources and Department of Agricultural Sciences on monitoring fungal activity in soils during growth and on imaging fungal hyphae architecture and soil microstructure. The researcher developed the methods for fungal growth and prepared all specimens for this work. A publication arising from this secondment is in preparation with authors from both University of Strathclyde and Università di Napoli.
Collaborator Contribution A colleague in the Department of Earth Science Environment conducted SEM of the soil-fungal specimens and of the control specimens (sand + lignocel). Colleagues in the Department of Agricultural Sciences performed the study monitoring fungal activity during growth, including measurements of pH, Electrical conductivity, respiration rate, erogosterol concentration.
Impact A journal publication is in preparation. Università di Napoli are a project partner on my Future Leaders Fellowship (MR/V02576/1).
Start Year 2019
 
Description Meeting with BAM Royal 
Form Of Engagement Activity A talk or presentation
Part Of Official Scheme? No
Geographic Reach Local
Primary Audience Industry/Business
Results and Impact Presentation of research outcomes from Engineering FUNGI to Royal BAM Director Alsadair Henderson. Mr Alasdair Henderson is particularly interested in the potential of fungal biogeotehcnologies in ground engineering. Based on research outcomes and in-depth discussion, Alasdair Henderson has provided support for a follow-on funding application to UKRI (FLF scheme) in the form of mentoring support to El Mountassir.
Year(s) Of Engagement Activity 2019,2020
 
Description Nature Inspired Solutions for the Built Environment Workshop 
Form Of Engagement Activity Participation in an activity, workshop or similar
Part Of Official Scheme? No
Geographic Reach International
Primary Audience Other audiences
Results and Impact Invited to speak at a workshop Nature inspired solutions for the built environment. > 50 people attended this multi-disciplinary workshop including academic and industry representatives.
Year(s) Of Engagement Activity 2021