Optically enabled micro-total analysis systems
Lead Research Organisation:
University of Dundee
Department Name: Electronic Engineering and Physics
Abstract
A micro-total analysis system, or a lab-on-a-chip, needs actuators capable of performing the key tasks of a miniature fluidic device; which include pumping, sorting, mixing and analysis. The inherent non-invasive nature of optical techniques, combined with their scalability, mean that they are ideally suited to performing these tasks. In this proposed body of work, I will in my primary drive, develop a range of such optically enabled microfluidic actuators. The first of these devices will be an all optical microfluidic sorting technique. This technique will allow biological or other matter to be sorted or fractionated by criteria that include, size, shape and internal density. A second push of my work will be towards the development of optical identification techniques suited to a lab-on-chip, base primarily on the use of the optical sorting technique to identify lowoccurrence species such as non-metastatic cancer cells in human blood. In third activity in the proposed work I will the develop an optically driven microfluidic pump capable of pumping small volumes of analyte in a controlled manner. I will adapt the sorting and pumping technologies to get microfluidic mixing. In parallel to all these approaches I will be developing microfluidic chambers, suited to the optical techniques being used; the end goal of which is to integrate two or more of my novel microfluidic techniques into a single device.
People |
ORCID iD |
Michael MacDonald (Principal Investigator) |
Publications
Dholakia K
(2007)
Optical Separation of Cells on Potential Energy Landscapes: Enhancement With Dielectric Tagging
in IEEE Journal of Selected Topics in Quantum Electronics
Dholakia K
(2007)
Cellular and colloidal separation using optical forces
Fischer P
(2007)
Two-photon ablation with 1278 nm laser radiation
in Journal of Optics A: Pure and Applied Optics
Milne G
(2007)
Fractionation of polydisperse colloid with acousto-optically generated potential energy landscapes.
in Optics letters
Smith R
(2007)
Colloidal sorting in dynamic optical lattices
in Journal of Optics A: Pure and Applied Optics
Description | New cell sorting technologies allowing for label free sorting. |
Exploitation Route | As part of a bioprocess for cell therapies or in the cell sorting for diagnostic purposes |
Sectors | Healthcare,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
Description | Currently our findings, (blood sorting techniques) are being used as the basis for the development of cell sorting techniques in the derivation of red blood cells from stem cells as an industrial replacement for a donor based blood transfusion service. This impact is at an early stage, but in essence our findings are being used to demonstrate a solution to a manufacturing bottleneck in cell therapy is possible. |
First Year Of Impact | 2010 |
Sector | Healthcare,Manufacturing, including Industrial Biotechology |
Impact Types | Policy & public services |
Description | Horizon fund |
Amount | £260,352 (GBP) |
Organisation | Government of Scotland |
Department | Scottish Funding Council |
Sector | Public |
Country | United Kingdom |
Start | 02/2011 |
End | 09/2016 |
Description | Manufacturing with Light |
Amount | £208,735 (GBP) |
Funding ID | EP/L022370/1 |
Organisation | Engineering and Physical Sciences Research Council (EPSRC) |
Sector | Public |
Country | United Kingdom |
Start | 05/2014 |
End | 11/2015 |
Description | Jo Mountford |
Organisation | University of Glasgow |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Optical and acoustic sorting of red blood cells for the industrialisation of red blood cell production |
Collaborator Contribution | Development of biological protocols for stem cell derived red blood cell as a replacement for the current donor based system. This collaboration includes collaboration with the Scottish National Blood transfusion service. |
Impact | Multidisciplinary: physics (optics and acoustics) with biology/medicine |
Start Year | 2010 |
Description | Nik Willoughby |
Organisation | Heriot-Watt University |
Country | United Kingdom |
Sector | Academic/University |
PI Contribution | Optical and acoustic technologies for blood sorting |
Collaborator Contribution | Process engineering |
Impact | Multidisciplinary: physics and engineering for bioprocessing in cell therapy |
Start Year | 2010 |
Title | Elliot Scientific Licence |
Description | Royalties agreement with Elliot Scientific for consultancy on optical trapping product |
IP Reference | |
Protection | Protection not required |
Year Protection Granted | |
Licensed | Yes |
Impact | Industry award |