New Imaging Technologies of synapses in physiology and pathology
Lead Research Organisation:
University of Edinburgh
Department Name: Centre for Clinical Brain Sciences
Abstract
Abstracts are not currently available in GtR for all funded research. This is normally because the abstract was not required at the time of proposal submission, but may be because it included sensitive information such as personal details.
People |
ORCID iD |
| Seth Grant (Principal Investigator) |
Publications
Broadhead MJ
(2016)
PSD95 nanoclusters are postsynaptic building blocks in hippocampus circuits.
in Scientific reports
Fernández E
(2017)
Arc Requires PSD95 for Assembly into Postsynaptic Complexes Involved with Neural Dysfunction and Intelligence.
in Cell reports
Frank RA
(2016)
NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation.
in Nature communications
Frank RAW
(2017)
Hierarchical organization and genetically separable subfamilies of PSD95 postsynaptic supercomplexes.
in Journal of neurochemistry
Kashiwagi Y
(2019)
Computational geometry analysis of dendritic spines by structured illumination microscopy.
in Nature communications
Wegner W
(2018)
In vivo STED microscopy visualizes PSD95 sub-structures and morphological changes over several hours in the mouse visual cortex.
in Scientific reports
| Title | Development of fluorescent reporter mouse lines for synapse imaging |
| Description | Development of various fluorescent reporter mouse lines for synapse imaging |
| Type Of Material | Model of mechanisms or symptoms - mammalian in vivo |
| Year Produced | 2016 |
| Provided To Others? | Yes |
| Impact | Helped to cement a new research collaboration with Hoffmann-La Roche entitled 'Measuring synaptic maps in vivo in response to pharmacological manipulation' (Mar 2016-Dec 2017, £100,000), with Seth Grant as Principal Investigator and, in follow up, as guest speaker at The Roche Neuroscience Research Forum, Basel, Switzerland, April 2018. |
| Title | Tools developed for the analysis of synGAP mutant mice |
| Description | For the analysis of synGAP mutant mice supplied to our JST-MRC partners, Prof. Okabe has developed the following new methods for quantitatively evaluating spine morphology: 1) Microscope system for in vivo two-photon imaging (three set-ups), super-resolution imaging system (one for SIM, the other for PALM imaging), equipment for EM connectome study (automated serial sectioning system plus scanning electron microscope). 2)Time-lapse synapse imaging in both cultured cells and in vivo, super-resolution imaging of synapses, EM reconstruction of synapses and neural connections. |
| Type Of Material | Technology assay or reagent |
| Year Produced | 2016 |
| Provided To Others? | No |
| Impact | No known impact yet |
| Description | synGAP and PSD95-GFP investigation |
| Organisation | University of Tokyo |
| Department | Department of Cellular Neurobiology |
| Country | Japan |
| Sector | Hospitals |
| PI Contribution | Professor Shigeo Okabe and Professor Seth Grant are collaborating further and investigating synGAP mutant mice and PSD95-GFP knock-in mice. |
| Collaborator Contribution | Mice supplied to Professor Okabe for further investigation on synGAP and PSD95 using super-resolution imaging of native synapses purified from mice expressing synaptic molecules tagged with photoactivatable fluorescent proteins. |
| Impact | Mice sent to Professor Okabe's laboratory. We have co-authored a paper: Kashiwagi Y., Higashi T., Obashi K., Sato Y., Komiyama N.H., Grant S.G.N. and Okabe S. (2019). Computational geometry analysis of dendritic spines by structured illumination microscopy. Nat. Commun. 10(1), 1285. |
| Start Year | 2016 |
| Description | 38th Annual Meeting of the Japan Neuroscience Society (Kobe 28-31/07/15) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Reported recent findings to a wider academic group |
| Year(s) Of Engagement Activity | 2015 |
| Description | I.M.A. workshop on neural imaging in neuroscience (Cambridge 21-23/03/2016) |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Study participants or study members |
| Results and Impact | Meeting with study participants to discuss results and future collaborations. Postgraduate students and early career level postdoctorial scientist participated in presentations. |
| Year(s) Of Engagement Activity | 2016 |
| Description | Seminar in Department of Cellular Neurobiology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo (July/2015) |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Regional |
| Primary Audience | Professional Practitioners |
| Results and Impact | Reported recent findings to a wider academic group |
| Year(s) Of Engagement Activity | 2015 |