Identification and characterization of cytochrome P450 enzymes critical to xenobiotic transformation in wildlife species (summary to follow)
Lead Research Organisation:
University of Manchester
Department Name: Chemistry
Abstract
Identification and characterization of cytochrome P450 enzymes critical to xenobiotic transformation in wildlife species
People |
ORCID iD |
| Andrew Munro (Primary Supervisor) |
Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| BB/P504920/1 | 03/01/2017 | 02/06/2021 | |||
| 1833407 | Studentship | BB/P504920/1 | 01/01/2017 | 30/06/2021 |
| Description | Expression of CYP1A protein form the Rainbow Trout has been achieved and a method developed for its isolation and purification. Structural analysis and characterization of the CYP1A protein has begun and data is being collated. Comparison with the Human counterpart is being carried out. Expression has also been achieved and method developed for the truncated CPR enzyme and an efficient purification protocol has been optimised. The enzyme will be then used as an effective redox partner enzyme for the purified CYP1A protein and, ultimately, other P450 enzymes of interest. Structural analysis and characterization of the CPR protein has begun and data is being collated. Comparison with the Human counterpart is being carried out. Expression of CYP3A126 protein from the Fathead Minnow has been achieved and a method developed for its isolation and purification. Structural analysis and characterization of the CYP3A126 protein has begun and data is being collated. Comparison with the Human counterpart is being carried out. The cytochrome P450 enzymes substrate profiles are being collected, this will include a range of known toxins. Following this metabolites will be collect and analysed via mass spectrometry. |
| Exploitation Route | The species chosen for this project represent a range of organisms which are already used as model organisms by research agencies, the government and industry. Cytochromes P450 can potentially be used as biochemical markers; since defining their function and mechanism of action (including their metabolites) on these known toxins they will provide an in vitro determination of harm and risk. The development and use of in vitro and predictive models to determine harm and risk of chemicals has increased recently, moving away from the traditional animal models as people become more aware of their impact on the environment and other species. The overarching aim of this project is to develop further understanding of important cytochrome P450 enzymes which may be conserved across species, Cytochrome P450 Enzymes from Environmentally Sensitive Organisms their function and the interaction of these enzymes with environmental toxins. The data gathered will provide deeper insights into the interactions of environmental toxins in particular, thus providing better environmental risk models for human activity. |
| Sectors | Chemicals,Environment,Manufacturing, including Industrial Biotechology,Pharmaceuticals and Medical Biotechnology |
| Description | 2019 International Conference on Cytochrome P450 |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Professional Practitioners |
| Results and Impact | Poster presentation at a conference to other academics and postgraduate students involved in Cytochrome P450 research. Poster gathered interest and discussion about my research. Potential collaborations and future contacts were made during the conference. Attended a range of talks which highlighted areas of interest in Cytochrome P450 research. |
| Year(s) Of Engagement Activity | 2019 |
| Description | Cytochrome P450 Biodiversity & Biotechnology 2018 Conference |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | International |
| Primary Audience | Other audiences |
| Results and Impact | Attended and presented a poster at the Cytochrome P450 Biodiversity & Biotechnology 2018 conference in York with delagates from various universitites and companies reseraching cytochrome P450s. The poster generated questions and discussions with members of the audience. |
| Year(s) Of Engagement Activity | 2018 |
| Description | Helped with PhD class for Intellectual Property |
| Form Of Engagement Activity | A talk or presentation |
| Part Of Official Scheme? | No |
| Geographic Reach | Local |
| Primary Audience | Postgraduate students |
| Results and Impact | 45 postgraduate PhD students attended the Innovation & Commercialisation of Research unit which I had been asked to help out. This involved me discussing my work history and discussing my experience of patents and intellectual property. This triggered questions and discussions as I circulated the room to aid them in their tasks. I was asked to return the following week to obsere their presentations and provide feedback. |
| Year(s) Of Engagement Activity | 2018 |
| Description | TPDG meeting |
| Form Of Engagement Activity | Participation in an activity, workshop or similar |
| Part Of Official Scheme? | No |
| Geographic Reach | National |
| Primary Audience | Other audiences |
| Results and Impact | Attended and presented a poster at the Tetra Pyrrole Discussion Group. Questions and discussions were made with members of the audience. |
| Year(s) Of Engagement Activity | 2018 |