Development of an in vitro model of the glomerular filtration barrier using cell sheet engineering
Lead Research Organisation:
University of Manchester
Department Name: School of Biological Sciences
Abstract
Complex in vitro models are starting to provide more defined tools for the study of specific substructural phenotypes for complex organs. At present there are no in vitro models of the glomerulus that demonstrate the range of characteristics indicative of the specific cell types within this complex structure, or enable its functionality as a filtration unit. Although cell lines exist, as single cell or co-culture systems, they do not offer the full genotype/phenotype of the glomerular filtration unit, nor do not they exist within a 3-dimensional architecture.
The development of such a model would enable the study of the basic biology of the glomerulus in an in vitro setting and how the various cell types interact within this structure to form a functional filtration barrier. This would also reduce the need for animal experimental models to study podocyte/glomerular biology. Furthermore, although recognised as a significant challenge, such a model could lend itself for association with nephron tubular cultures enabling the formation of an integrated in vitro model of the kidney.
The remit of the project would be to model the organotypic structure and function of epithelial cells (podocytes) within the glomerulus in association with the glomerular endothelium, using 3D culture technologies, fluidics/microfluidics and co-culture. Cell sheet engineering, a technique already established within the group at the University of Manchester, will be available to aid the assembly of the model. The successful candidate would gain valuable first-hand experience in building complex in vitro models, the use of various microscopy techniques, and a deep background knowledge of kidney biology/physiology and pathophysiology
The development of such a model would enable the study of the basic biology of the glomerulus in an in vitro setting and how the various cell types interact within this structure to form a functional filtration barrier. This would also reduce the need for animal experimental models to study podocyte/glomerular biology. Furthermore, although recognised as a significant challenge, such a model could lend itself for association with nephron tubular cultures enabling the formation of an integrated in vitro model of the kidney.
The remit of the project would be to model the organotypic structure and function of epithelial cells (podocytes) within the glomerulus in association with the glomerular endothelium, using 3D culture technologies, fluidics/microfluidics and co-culture. Cell sheet engineering, a technique already established within the group at the University of Manchester, will be available to aid the assembly of the model. The successful candidate would gain valuable first-hand experience in building complex in vitro models, the use of various microscopy techniques, and a deep background knowledge of kidney biology/physiology and pathophysiology
People |
ORCID iD |
Rachel Lennon (Primary Supervisor) | |
Nikki-Maria Koudis (Student) |
Studentship Projects
Project Reference | Relationship | Related To | Start | End | Student Name |
---|---|---|---|---|---|
BB/P504348/1 | 01/07/2017 | 30/06/2021 | |||
1905262 | Studentship | BB/P504348/1 | 01/07/2017 | 30/06/2021 | Nikki-Maria Koudis |
Description | Development of an in vitro model of the filtration barrier within the kidney using cell sheet engineering. This can further be used as a tool to understand how the barrier functions both in health and disease |
Exploitation Route | The model can be used to study the basement membrane within the filtration barrier of the kidney. |
Sectors | Pharmaceuticals and Medical Biotechnology |
Description | Kidneys for Life - Travel bursary |
Amount | £800 (GBP) |
Organisation | Kidneys for Life |
Sector | Charity/Non Profit |
Country | United Kingdom |
Start | 09/2018 |
End | 11/2018 |
Description | CASE studentship |
Organisation | GlaxoSmithKline (GSK) |
Department | Safety Assessment GSK |
Country | United Kingdom |
Sector | Private |
PI Contribution | Regular meetings and presentations to collaborator throughout the duration of the PhD. This led to good questions and ideas for continued research from the pharmaceutical company. |
Collaborator Contribution | Advice and feedback to the ongoing project. |
Impact | Short term dry lab project at the beginning of the PhD and the potential of another short term project in the next year. Good feedback and ideas from the collaborator which have been taken on board for the duration of the PhD. |
Start Year | 2017 |
Description | BioDiscovery public engagemnet |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Schools |
Results and Impact | 'BioDiscovery' event targeted at secondary school students (years 7-10) to enable them to discover more about the Biological Sciences |
Year(s) Of Engagement Activity | 2020 |
Description | Volunteer at European Researcher's Night |
Form Of Engagement Activity | Participation in an activity, workshop or similar |
Part Of Official Scheme? | No |
Geographic Reach | Local |
Primary Audience | Public/other audiences |
Results and Impact | European Researcher's night at Manchester Museum which is targeted to an adult audience. We ran a kidney stand which enabled people to come and ask us questions related to our research and thus we were able to spread awareness to the general public. |
Year(s) Of Engagement Activity | 2017,2018,2019 |