Mapping aggregation-prone regions induced by extensional flow
Lead Research Organisation:
University of Leeds
Department Name: Sch of Molecular & Cellular Biology
Abstract
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Studentship Projects
| Project Reference | Relationship | Related To | Start | End | Student Name |
|---|---|---|---|---|---|
| BB/S507234/1 | 30/09/2018 | 31/12/2022 | |||
| 2111955 | Studentship | BB/S507234/1 | 30/09/2018 | 31/12/2022 | Samantha Lawrence |
| NE/W503125/1 | 31/03/2021 | 30/03/2022 | |||
| 2111955 | Studentship | NE/W503125/1 | 30/09/2018 | 31/12/2022 | Samantha Lawrence |
| Title | Protein Pressure! |
| Description | I collaborated with the Directors of LYNNEBEC, a performance company based in Birmingham, to run an educational dance class, depicting my PhD work in the form of dance. My class was Week 6 of the 'AlgoRHYTHM! From Home' project. The classes in this project were like 'science Zumba' designed to engage the public with world-leading science in a dance routine. This was given via Zoom engaging participants over the world. The science was generalised for a non-scientific audience. The routine followed the background of biopharmaceuticals, why they have a specific protein fold (to be active as medicines), how I use lasers and mass spectrometry to confirm the protein fold, and how I use hydrodynamic forces in my PhD to deliberately put pressure on the protein to identify how we can make it less susceptible to unfolding, and the impact this research could have for making medicine better in the future. |
| Type Of Art | Performance (Music, Dance, Drama, etc) |
| Year Produced | 2020 |
| Impact | The class was performed by approximately 20 participants of the general public. |
| URL | https://www.instagram.com/tv/CEcQ6ZrH7l6/?utm_source=ig_web_copy_link |
| Description | Using structural MS techniques for investigating aggregation interfaces of biopharmaceuticals |
| Organisation | AstraZeneca |
| Department | MedImmune |
| Country | United Kingdom |
| Sector | Private |
| PI Contribution | I have applied expertise in protein unfolding manipulation under hydrodynamic flow to the provided propriety bipharmaceuticals, and used FPOP coupled to Mass Spectrometry to map the solvent-accessible regions on the proteins. I have updated the team at AstraZeneca on my research a minimum of 4 times per year so far. |
| Collaborator Contribution | They have provided a supply of biopharmaceuticals. I have also requested the production of 2 additional evolved proteins, which AstraZeneca have kindly provided me with. Their feedback at my presentation meetings have been invaluable for the progression of my project. |
| Impact | N/A |
| Start Year | 2018 |