Imaging fluorescence from cells in 5-dimensions: x, y, wavelength, lifetime, and anisotropy.
Lead Research Organisation:
Nottingham Trent University
Department Name: School of Science & Technology
Abstract
Fluorescent probes are widely used to study the location and function of biomolecules in cells. Using current fluorescence methods, the behavior of molecules within cells can be studied. Examples include: clustering, rotational motion, and intermolecular distance. These three examples are studied by measuring fluorescence lifetime and anisotropy. Simultaneous monitoring of the behavior of many types of molecules in cells over an extended area is an important unsolved physical problem. This proposal seeks to develop, study, and use a new approach to fluorescence imaging combining spectroscopy, fluorescence lifetime, and anisotropy with a goal of studying the behavior of many types of molecules in cells simultaneously. This will produce images with x and y spatial dimensions and fluorescence emission wavelength, fluorescence lifetime, and fluorescence anisotropy. The research has three parts: construction of new instrumentation, tests on defined mixtures of fluorescent compounds in a solution, and study of an important cell surface receptor. Successful completion of the study will provide biologists with new ways to study clustering, motion, and intermolecular distances of multiple molecular species in the field of a microscope.
Publications
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Ghafari H
(2009)
Confocal detection of planar homogeneous and heterogeneous immunosorbent assays.
in Journal of biomedical optics
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Hanley Q
(2008)
Spectrally resolved fluorescent lifetime imaging
in Journal of The Royal Society Interface
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N/a Zhou
(2008)
Frequency Domain Imaging of Lifetime and Anisotropy Spectra
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Zhou Y
(2009)
Imaging lifetime and anisotropy spectra in the frequency domain.
in Journal of microscopy
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Zhou Y
(2011)
Global analysis of dynamic fluorescence anisotropy by a polarized phasor approach.
in Journal of fluorescence
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Zhou Y
(2013)
Processing of fluorescence lifetime image using modified phasor approach: homo-FRET from the acceptor.
in Journal of fluorescence
Description | This has had by facilitating knowledge and skills transfer between countries. Specifically, Dr Yanzhou Zhou returned to China and continued to publish in the area. |
First Year Of Impact | 2010 |
Sector | Other |
Impact Types | Cultural |